LOCUS       sequence-258703-        5681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5681)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACVR2A
FEATURES             Location/Qualifiers
     source          1..5681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1287..1421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1324..1343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1378..1397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1450..1615
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1799..2253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1886..1905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2095..2116
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2279..2383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2384..2452,2453..3244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2602..2621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3415..4003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3904..3923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4157..4174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4308..4532
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4599..5132)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5302..5307,5308..5324,5325..5330))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgc cactgcagtt attagaagtg
      241 aaagcaaggg gaagatttgg ttgtgtctgg aaagcccagt tgcttaacga atatgtggct
      301 gtcaaaatat ttccaataca ggacaaacag tcatggcaaa atgaatacga agtctacagt
      361 ttgcctggaa tgaagcatga gaacatatta cagttcattg gtgcagaaaa acgaggcacc
      421 agtgttgatg tggatctttg gctgatcaca gcatttcatg aaaagggttc actatcagac
      481 tttcttaagg ctaatgtggt ctcttggaat gaactgtgtc atattgcaga aaccatggct
      541 agaggattgg catatttaca tgaggatata cctggcctaa aagatggcca caaacctgcc
      601 atatctcaca gggacatcaa aagtaaaaat gtgctgttga aaaacaacct gacagcttgc
      661 attgctgact ttgggttggc cttaaaattt gaggctggca agtctgcagg cgatacccat
      721 ggacaggttg gtacccggag gtacatggct ccagaggtat tagagggtgc tataaacttc
      781 caaagggatg catttttgag gatagatatg tatgccatgg gattagtcct atgggaactg
      841 gcttctcgct gtactgctgc agatggacct gtagatgaat acatgttgcc atttgaggag
      901 gaaattggcc agcatccatc tcttgaagac atgcaggaag ttgttgtgca taaaaaaaag
      961 aggcctgttt taagagatta ttggcagaaa catgctggaa tggcaatgct ctgtgaaacc
     1021 attgaagaat gttgggatca cgacgcagaa gccaggttat cagctggatg tgtaggtgaa
     1081 agaattaccc agatgcagag actaacaaat atttgacagt aaaggtggat acggatccga
     1141 attcgagctc cgtcgacaag cttgtcgaga agtactagag gatcataatc agccatacca
     1201 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1261 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1381 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta
     1441 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     1501 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     1561 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
     1621 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
     1681 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta
     1741 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac
     1801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac
     2101 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2161 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2221 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     2281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2341 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2821 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4081 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4141 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     4201 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     4261 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     4321 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     4381 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     4441 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     4501 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     4561 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     4621 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     4681 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     4741 ttgagcagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     4801 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     4861 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     4921 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     4981 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     5041 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     5101 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     5161 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     5221 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     5281 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     5341 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     5401 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     5461 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag
     5521 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg
     5581 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat
     5641 agttctagtg gttggctacg tatactccgg aatattaata g
//
