LOCUS       sequence-25872-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV/TO 
            promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO Neo DEST (685-3)
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      32..167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    207..328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(218..237)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     272..291
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             400..633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
       61 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
      121 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
      181 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
      241 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      301 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
      361 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
      421 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
      481 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
      541 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
      601 cttgtcttcg ttgggagtga gctagccctt ccagtccccc cttttctttt aaaaagtgg
//
