LOCUS       sequence-258730-        8792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable
            His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Deaconescu AM, Darst SA
  TITLE     Crystallization and preliminary structure determination of 
            Escherichia coli Mfd, the transcription-repair coupling factor.
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt 
            12):1062-4. doi: 10.1107/S1744309105035876. Epub 2005 Nov 24.
  PUBMED    16511235
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr Sect F Struct Biol Cryst Commun."; date: "2005-12-1"; 
            volume: "61(Pt 12):1062-4. doi"; pages: " 10.1107/S1744309105035876.
            Epub 2005 Nov 24"
COMMENT     Sequence Label: pAD6
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4984..5003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5110..5133
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8636..8653
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8706..8724)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8720..8767
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     5101 agcagcggcc tggaagttct gttccagggg ccccatatgg ctagcatgcc tgaacaatat
     5161 cgttatacgc tgcccgtcaa agcgggtgag cagcgtctgc tgggcgagtt aaccggcgca
     5221 gcctgtgcaa cgctggtagc ggaaattgcc gaacgtcacg ccggtccggt ggtactcatt
     5281 gcaccagata tgcaaaatgc tctgcgtttg catgatgaaa tcagccagtt taccgatcaa
     5341 atggtgatga atctggcgga ctgggaaact cttccctacg acagtttttc gcctcatcag
     5401 gacattatct cctcgcgcct ttccaccctt taccagctac cgacgatgca gcgtggcgta
     5461 ctgattgttc cggtgaatac gcttatgcag cgcgtttgcc cacacagttt tctccacggt
     5521 catgcgctgg tgatgaaaaa aggtcagcgc ctgtcacgag atgcattacg aacccaactg
     5581 gacagcgccg gttatcgcca tgttgaccag gtgatggagc acggcgaata cgccacgcgc
     5641 ggcgcgttgc tggatctctt cccgatgggg agtgagctgc cttatcgtct tgatttcttt
     5701 gatgatgaaa tcgacagcct gcgggtgttt gacgtcgaca gccagcgcac gctggaggaa
     5761 gtagaagcga tcaatctgct gcccgcgcac gaatttccga ccgataaagc ggcaattgaa
     5821 ctgttccgca gccagtggcg cgataccttc gaagtgaagc gcgatccaga acatatttac
     5881 cagcaagtga gtaaaggcac attacctgcc gggatcgagt actggcagcc attgttcttc
     5941 agcgaaccac tgccgccgct gttcagttat ttccctgcca ataccttgct cgtgaatact
     6001 ggcgatctgg aaaccagtgc cgaacgtttc caggctgaca cgctggcgcg ttttgagaat
     6061 cgcggcgtcg atccgatgcg cccgctgttg ccaccacaat cgctctggct gcgggtggac
     6121 gagctcttct cagagctgaa aaactggccc cgggtgcagc taaaaactga acatttaccg
     6181 acaaaagccg cgaatgccaa tttaggtttc cagaaactgc cagacctggc cgttcaggca
     6241 caacaaaaag cgccgctgga tgcgctgcgt aagttcctcg agactttcga cggtccggtg
     6301 gtgttctcgg tagaaagtga aggtcgccgt gaagcgctgg gtgaactgct cgcacgaatt
     6361 aaaattgctc cgcaacgcat tatgcgtctt gatgaagcca gcgaccgtgg gcgttatctg
     6421 atgattggcg ctgccgaaca tggttttgtc gatacggtgc gtaatctggc gctgatttgc
     6481 gaaagcgatc tgctcggtga acgcgttgcc cgtcgtcgtc aggattctcg ccgcaccatc
     6541 aaccccgata cactgatccg taaccttgcg gaactgcata ttggtcagcc ggtggtccat
     6601 ctggagcacg gcgtcggtcg ttatgccgga atgaccacgc tggaagcggg tggcattact
     6661 ggcgagtatc tgatgctcac ctatgccaac gacgccaaac tgtatgttcc ggtgtcgtca
     6721 ctgcatctga ttagccgtta cgcaggtggc gcggaagaaa acgccccgct gcataaactt
     6781 ggcggcgatg cgtggtcacg cgcgcggcag aaagcggcgg aaaaagtgcg tgatgtggcg
     6841 gcggaattgc tggatatcta cgcgcaacgc gccgccaaag agggcttcgc gtttaaacac
     6901 gatcgtgagc agtatcagtt gttctgcgac agcttcccgt ttgaaaccac gccggatcag
     6961 gcgcaggcca ttaatgcggt acttagcgac atgtgtcagc cgctggcaat ggatcgtctg
     7021 gtgtgcggcg atgttggctt tggtaaaaca gaagtggcga tgcgcgcagc tttcctggca
     7081 gtagataacc acaagcaggt ggcggtgctg gtgcctacca cccttctcgc gcagcagcat
     7141 tacgacaact tccgcgaccg tttcgccaac tggccggtac gtatcgaaat gatctcccgt
     7201 ttccgcagcg ccaaagagca gacgcaaatc cttgcggaag tggcggaagg gaaaatcgat
     7261 attctgatcg gtacgcacaa actgctgcaa agtgacgtca agtttaaaga tttaggcctg
     7321 ctgattgtcg atgaagaaca ccgcttcggg gtgcgtcata aagagcgcat taaagcgatg
     7381 cgcgcgaacg tggatattct gacgcttact gcaacgccga tcccacgtac gctgaatatg
     7441 gcaatgagcg gaatgcgtga cctgtcgatt atcgccacgc cgcccgcccg tcgtctggca
     7501 gttaaaacct ttgtccgtga gtatgacagc atggtggtcc gggaggcgat cctgcgtgaa
     7561 attttgcgcg gaggacaggt ttattatctc tacaatgatg tggaaaacat tcagaaagcc
     7621 gccgaacggc tggcagaact ggtgccagaa gcgcggatcg ccatcggtca cgggcagatg
     7681 cgcgagcgcg aactggaacg ggtgatgaat gatttccatc atcaacgttt caacgtgctg
     7741 gtttgtacaa ccattatcga aaccgggatc gacatcccga cagccaacac tattatcatt
     7801 gaacgcgcgg atcacttcgg tctggcgcag ctgcaccagt tacgcggtcg cgtcggacgt
     7861 tcgcatcatc aggcatatgc atggttgctg acaccgcatc caaaagcgat gactaccgat
     7921 gcacaaaaac gtcttgaagc aattgcctcg ctggaagatc tcggggcagg ttttgcgctg
     7981 gcaacgcacg atctggagat tcgcggcgcg ggtgaactgc ttggcgaaga acaaagcggc
     8041 tcaatggaaa ccatcggttt ctcgctgtat atggagttgc tggaaaacgc cgtcgatgca
     8101 ctgaaagccg gacgcgagcc gtcgctggaa gatctcacca gccagcaaac agaagtcgag
     8161 ctgcggatgc cgtcgctatt gccagatgat ttcatccctg acgtgaacac gcgtctgtcg
     8221 ttctacaaac gtattgccag cgccaaaacg gaaaacgaac tggaagagat caaagtcgag
     8281 cttatcgatc gcttcggcct gctgccagat ccggcgcgta ccctgctgga tattgcccgt
     8341 ctgcgccagc aagcgcagaa actggggatc aggaagctgg aaggtaatga gaaaggcggg
     8401 gtgatcgaat ttgccgagaa gaatcacgtt aatccggcct ggttgattgg tttgctgcaa
     8461 aaacagccgc agcattaccg ccttgatggt ccgacgcgcc tgaaatttat tcaggatttg
     8521 agtgagcgga aaacgcgtat cgaatgggta cgccagttta tgcgtgaact ggaagagaac
     8581 gcgatcgctt aatgagaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca
     8641 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     8701 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     8761 ttttttgctg aaaggaggaa ctatatccgg at
//
