LOCUS       sequence-258738-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of SIV-VLPs containing Vpx protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C,
            Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
  TITLE     Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 
            10.1038/s41564-019-0592-5. Epub 2019 Oct 21.
  PUBMED    31636416
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0592-5"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2044-2051"
COMMENT     Sequence Label: pSIV-D3psi/delta env/delta Vif/delta Vpr
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7679..7813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7716..7735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7770..7789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7865..7969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7970..8038,8039..8830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8188..8207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9001..9589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9490..9509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9675..9691
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9675..9691
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgaggccg caagcttggc ctccggttgc aggtaagtgc aacacaaaaa
      721 agaaatagct gtcttgttat ccaggaaggg ataataagat agagtgggag atgggcgcga
      781 gaaactccgt cttgtcaggg aagaaagcag atgaattaga aaaaattagg ctacgacccg
      841 gcggaaagaa aaagtacatg ttgaagcatg tagtatgggc agcaaatgaa ttagatagat
      901 ttggattagc agaaagcctg ttggagaaca aagaaggatg tcaaaaaata ctttcggtct
      961 tagctccatt agtgccaaca ggctcagaaa atttaaaaag cctttataat actgtctgcg
     1021 tcatctggtg cattcacgca gaagagaaag tgaaacacac tgaggaagca aaacagatag
     1081 tgcagagaca cctagtggtg gaaacaggaa cagcagaaac tatgccaaaa acaagtagac
     1141 caacagcacc atctagcggc agaggaggaa attacccagt acaacaaata ggtggtaact
     1201 atgtccacct gccattaagc ccgagaacat taaatgcctg ggtaaaattg atagaggaaa
     1261 agaaatttgg agcagaagta gtgccaggat ttcaggcact gtcagaaggc tgcaccccct
     1321 atgacattaa tcagatgtta aattgtgtgg gagaccatca agcggctatg cagattatca
     1381 gagatattat aaatgaggag gctgcagatt gggacttgca gcacccacaa ccagctccac
     1441 aacaaggaca gcttagggag ccgtcaggat cagatattgc aggaacaact agttcagtag
     1501 atgaacaaat ccagtggatg tacagacaac agaaccccat accagtaggc aacatttaca
     1561 ggagatggat ccaactgggg ttgcaaaaat gtgtcagaat gtataaccca acaaacattc
     1621 tagatgtaaa acaagggcca aaagagccat ttcagagcta tgtagacagg ttctacaaaa
     1681 gcttaagagc agaacaaaca gatgcagcag taaagaattg gatgactcaa acactgctga
     1741 ttcaaaatgc taacccagat tgcaagctag tgctgaaggg gctgggtgtg aatcccaccc
     1801 tagaagaaat gctgacggct tgtcaaggag tagggggacc aggacagaag gctagattaa
     1861 tggcagaagc cctgaaagag gccctcgcac cagtgccaat cccttttgca gcagcccaga
     1921 agaggggacc aagaaagcca attaagtgtt ggaattgtgg gaaggaggga cactctgcaa
     1981 ggcaatgcag agccccaaga agacagggat gctggaaatg tggaaaaatg gaccatgtta
     2041 tggccaaatg cccagacaga caggcgggtt ttttaggcct tggtccatgg ggaaagaagc
     2101 cccgcaattt ccccatggct caagtgcatc aggggctgac gccaactgct cccccagagg
     2161 acccagctgt ggatctgcta aagaactaca tgcagttggg caagcagcag agagaaagca
     2221 gagagaagcc ttacaaggag gtgacagagg atttgctgca cctcaattct ctctttggag
     2281 gagaccagta gtcactgctc atattgaagg acagcctgta gaagtattat tggatacagg
     2341 ggctgatgat tctattgtaa caggaataga gttaggtcca cattataccc caaaaatagt
     2401 aggaggaata ggaggtttta ttaatactaa agaatacaaa aatgtaaaaa tagaagtttt
     2461 aggcaaaagg attaaaggga caatcatgac aggggacact ccgattaaca tttttggtag
     2521 gaatttgcta acagctctgg ggatgtctct aaatcttccc atagctaagg tagagcctgt
     2581 aaaagtcacc ttaaagccag gaaaggttgg accaaaattg aagcagtggc cattatcaaa
     2641 agaaaagata gttgcattaa gagaaatctg tgaaaagatg gaaaaggatg gtcagttgga
     2701 ggaagctccc ccgaccaatc catacaacac ccccacattt gccataaaga aaaaagataa
     2761 gaacaaatgg agaatgctga tagattttag ggaactaaat agggtcactc aggactttac
     2821 agaagtccaa ttaggaatac cacaccctgc aggactagca aaaaggaaaa ggattacagt
     2881 actggatata ggtgatgcat atttctccat acctctagat gaagaattta ggcagtacac
     2941 tgcctttact ttaccatcag taaataatgc agagccagga aaacgataca tttataaggt
     3001 tctgcctcag ggatggaagg ggtcaccagc catcttccaa tacactatga gacatgtgct
     3061 agaacccttc aggaaggcaa atccagatgt gaccttagtc cagtatatgg atgacatctt
     3121 aatagctagt gacaggacag acctggaaca tgacagggta gttttacagc taaaggaact
     3181 cttaaatagc atagggttct ctaccccaga agagaaattc caaaaagatc ccccatttca
     3241 atggatgggg tacgaattgt ggccgacaaa atggaagttg caaaagatag agttgccaca
     3301 aagagagacc tggacagtga atgatataca gaagttagta ggagtattaa attgggcagc
     3361 tcaaatttat ccaggtataa aaaccaaaca tctctgtagg ttaattagag gaaaaatgac
     3421 tctaacagag gaagttcagt ggactgagat ggcagaagca gaatatgagg aaaataagat
     3481 aattctcagt caggaacaag aaggatgtta ttaccaagaa ggcaagccat tagaagccac
     3541 ggtaataaag agtcaggaca atcagtggtc ttataaaatt caccaagaag acaaaatact
     3601 gaaagtagga aaatttgcaa agataaagaa tacacatacc aatggagtta gactattagc
     3661 acatgtaata cagaaaatag gaaaggaagc aatagtgatc tggggacagg tcccaaaatt
     3721 ccacttacca gttgagaggg atgtatggga acagtggtgg acagactatt ggcaggtaac
     3781 ctggataccg gagtgggatt ttatctcaac gccaccacta gtaagattag tcttcaatct
     3841 agtgaaggac cctatagagg gagaagaaac ctattataca gatggatcat gtaataaaca
     3901 gtcaaaagaa gggaaagcag gatatatcac agataggggc aaagacaaag taaaagtgtt
     3961 agaacagact actaatcaac aagcagaatt agaagcattt ctcatggcat tgacagactc
     4021 agggccaaag acaaatatta tagtagattc acaatatgtt atgggaataa taacaggatg
     4081 ccctacagaa tcagagagca ggctagttaa ccaaataata gaagaaatga ttaaaaagtc
     4141 agaaatttat gtagcatggg taccagcaca caaaggtata ggaggaaacc aagaaataga
     4201 ccacctagtt agtcagggga ttagacaagt tctcttcttg gaaaagatag agccagcaca
     4261 agaagaacat gataaatacc atagtaatgt aaaagaattg gtattcaaat ttggattacc
     4321 cagaatagtg gccagacaga tagtagacac ctgtgataaa tgtcatcaga aaggagaagc
     4381 tatacatggg caggtaaatt cagatctagg gacttggcaa atggactgta cccatctaga
     4441 aggaaaaata gtcatagttg cagtacatgt agctagtgga ttcatagaag cagaagtaat
     4501 tccacaagag acaggaagac agacagcact atttctgtta aaattggcag gcagatggcc
     4561 tattacacat ctacacacag ataatggtgc taactttgcc tcgcaagaag taaagatggt
     4621 tgcatggtgg gcagggatag agcacacctt tggggtacca tacaatccac agagtcaggg
     4681 agtagtggaa gcaatgaatc accacctgaa aaatcaaata gatagaatca gggaacaagc
     4741 aaattcagta gaaaccatag tattaatggc agttcattgc atgaatttta aaagaagggg
     4801 aggaataggg gatatgactc cagcagaaag attaattaac atgatcacta cagaacaaga
     4861 aatacaattt caacaatcaa aaaactcaaa atttaaaaat tttcgggtct attacagaga
     4921 aggcagagat caactgtgga agggacccgg tgagctattg tggaaagggg aaggagcagt
     4981 catcttaaag gtagggacag acattaaggt agtacccaga agaaaggcta aaattatcaa
     5041 agattacgga ggaggaaaag aggtggatag cagttcccac atggaggata ccggagaggc
     5101 tagagaggtg gcatagcctc ataaaatatc tgaaatagaa aactaaagat ctacaaaagg
     5161 tttgctatgt gccccatttt aaggtcggat gggcatggtg gacctgcagc agagtaatct
     5221 tccccctaca ggaaggaagc catttagaag tacaagggta ttggcatttg acaccagaaa
     5281 gagggtggct cagtacttat gcagtgagga taacatggta ctcaaggaac ttttggacag
     5341 atgtaacacc agactatgca gacattttac tgcatagcac ttatttccct tgctttacag
     5401 cgggagaagt gagaagggcc atcaggggag aacaactgct gtcttgctgc aagttcccga
     5461 gagcacatag gtaccaggta ccaagcctac agtacttagc actaaaagta gtaagcgatg
     5521 tcagatccca gggagagaat cccacctgga aacagtggag aagagacaat aggagaagcc
     5581 ttcgaatggc taaacagaac agtagaggag ataaacagag aggcagtaaa ccacctacca
     5641 agggagctga ttttccaggt ttggcaaagg tcttgggaat actggcatga tgaacaaggg
     5701 atgtcacaaa gctatgtaaa atacagatac ttgtgtttaa tgcaaaaggc tttatttatg
     5761 cattgcaaga aaggctgtag atgtctaggg gaaggacacg gggcaggagg atggagacca
     5821 ggacctcctc ctcctccccc tccaggacta gcataatagg aagaaagacc tccggaaaat
     5881 gaaggcccac aaagggaacc atgggatgaa tgggtagtgg aggtttagga agaattgaaa
     5941 gaagaagctt taaaacattt tgatcctcgc ttgctaactg cacttggtaa tcatatctat
     6001 aatagacatg gagacaccct tgagggagca ggagaactca ttagaatcct ccaacgagcg
     6061 ctcttcatgc attttagagg cggatgcaac cactccagaa tcggccaacc tgggggagga
     6121 aatcctctct caactatacc gccctcttga ggcgtgctat aacacatgct attgtaaaaa
     6181 gtgttgctac cattgccagt tttgttttct taaaaaggga ttggggatat gttatgagca
     6241 gtcacgaaag agaagaagaa ctccgaaaaa ggctaaggct aatacatctt ctgcatcaaa
     6301 caagtaagta tgccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     6361 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagtatcac catgagtgca
     6421 gaggtggcag aactgtatcg attggagttg ggagattata aattagtaga gatcactccg
     6481 attggcttgg cccccacaga tgtgaagagg tacactactg gtggcacctc aagaaataaa
     6541 agaggggtct ttgtgctagg gttcttgggt tttctcgcaa cggcaggttc tgcaatgggc
     6601 gcggcgtcgt tgacgctgac cgctcagtcc cggactttat tggctgggat agtgcagcaa
     6661 cagcaacagc tgttggacgt ggtcaagaga caacaagaat tgttgcgact gaccgtctgg
     6721 ggaacaaaga acctccagac tagggtcact gccatcgaga agtacttaaa ggaccaggcg
     6781 cagctaaatg cttggggatg tgcgtttaga caagtctgcc acactactgt accatggcca
     6841 aatgcaagtc taacaccaga ctggaacaat gatacttggc aagagtggga gcgaaaggtt
     6901 gacttcttgg aggaaaatat aacagccctc ctagaagagg cacaaattca acaagagaag
     6961 aacatgtatg aattacaaaa gttgaatagc tgggatgtgt ttggcaattg gtttgacctt
     7021 gcttcttgga taaagtatat acaatatgga atttatgtag ttgtaggagt aatactgtta
     7081 agaatagtga tctatatagt acaaatgcta gctaagttaa ggcaggggta taggccagtg
     7141 ttctcttccc caccctctta tttccagtag actcataccc aacaggaccc ggcactgcca
     7201 accagagaag gcaaagaagg agacggtgga gaaggcggtg gcaacagctc ctggccttgg
     7261 cagatagaat atattcattt cctgatccgc caactgatac gcctcttgac ttggctattc
     7321 agcaactgca gaaccttgct atcgagagca taccagatcc tccaaccaat actccagagg
     7381 ctctctgcga ccctacgaag ggttcgagaa gtcctcagga ctgaactgac ctacctacaa
     7441 tatgggtgga gctatttcca tgaggcggtc caagccggct ggagatctgc gacagaaact
     7501 cttgcgggcg cgtggagaga cttatgggag actcttagga gaggtggaag atggatcctc
     7561 gcaatcccta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7621 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7801 tcatgtctgg atcgaattcg atatcatcat tgggacgtca ggtggcactt ttcggggaaa
     7861 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7921 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7981 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8041 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8101 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8161 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8221 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8281 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8341 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8401 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8461 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8521 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8581 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8641 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8701 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8761 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8821 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8881 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8941 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9001 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9061 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gggccctagg
     9661 ccggcctcgg ccgctctaga actagtggat ccgggccgct ctagagagct tggcccattg
     9721 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
     9781 ccatgtt
//
