LOCUS       sequence-258739-       10360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p8.9 NdeltaSB bearing P90A mutant capsid sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   De Iaco A, Luban J
  TITLE     Inhibition of HIV-1 infection by TNPO3 depletion is determined by 
            capsid and detectable after viral cDNA enters the nucleus.
  JOURNAL   Retrovirology. 2011 Dec 6;8:98. doi: 10.1186/1742-4690-8-98.
  PUBMED    22145813
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Retrovirology."; date: "2011-12-6"; volume: "8:98. doi"; pages: " 
            10.1186/1742-4690-8-98"
COMMENT     Sequence Label: p8.9 NdeltaSB P90A
FEATURES             Location/Qualifiers
     source          1..10360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..27
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(97..117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        102..431
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      282..417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        618..921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        922..1125
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1076..1096
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1122..1146
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1401..2903
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGA
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2696..5707
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5392..5509
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6281..6514
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6699..6743
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7037..7063
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(7686..7704)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7687..7704)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7994..8013)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8227..8246)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8346..8368
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        8492..8596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8597..8665,8666..9457)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8815..8834)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9628..10216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10117..10136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
       61 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
      121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
      421 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
      481 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
      541 tttagtgcgc cagatcctag ttattaatag taatcaatta cggggtcatt agttcatagc
      601 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      661 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      721 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      781 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      841 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      901 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      961 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     1021 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     1081 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     1141 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     1201 tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc caagagtgac
     1261 gtaagtaccg cctatagagt ctataggccc acccccttgg cttcttatgc gacggatcga
     1321 tcccgtaata agcttcgagg tccgcggccg gccgcgttga cgcgcacggc aagaggcgag
     1381 gggcggccgc ctggtgagag atgggtgcga gagcgtcggt attaagcggg ggagaattag
     1441 ataaatggga aaaaattcgg ttaaggccag ggggaaagaa acaatataaa ctaaaacata
     1501 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctt ttagagacat
     1561 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1621 aacttagatc attatataat acaatagcag tcctctattg tgtgcatcaa aggatagatg
     1681 taaaagacac caaggaagcc ttagataaga tagaggaaga gcaaaacaaa agtaagaaaa
     1741 aggcacagca agcagcagct gacacaggaa acaacagcca ggtcagccaa aattacccta
     1801 tagtgcagaa cctccagggg caaatggtac atcaggccat atcacctaga actttaaatg
     1861 catgggtaaa agtagtagaa gagaaggctt tcagcccaga agtaataccc atgttttcag
     1921 cattatcaga aggagccacc ccacaagatt taaataccat gctaaacaca gtggggggac
     1981 atcaagcagc catgcaaatg ttaaaagaga ccatcaatga ggaagctgca gaatgggata
     2041 gattgcatcc agtgcatgca ggtgccattg caccaggcca gatgagagaa ccaaggggaa
     2101 gtgacatagc aggaactact agtacccttc aggaacaaat aggatggatg acacataatc
     2161 cacctatccc agtaggagaa atctataaaa gatggataat cctgggatta aataaaatag
     2221 taagaatgta tagccctacc agcattctgg acataagaca aggaccaaag gaacccttta
     2281 gagactatgt agaccgattc tataaaactc taagagccga gcaagcttca caagaggtaa
     2341 aaaattggat gacagaaacc ttgttggtcc aaaatgcgaa cccagattgt aagactattt
     2401 taaaagcatt gggaccagga gcgacactag aagaaatgat gacagcatgt cagggagtgg
     2461 ggggacccgg ccataaagca agagttttgg ctgaagcaat gagccaagta acaaatccag
     2521 ctaccataat gatacagaaa ggcaatttta ggaaccaaag aaagactgtt aagtgtttca
     2581 attgtggcaa agaagggcac atagccaaaa attgcagggc ccctaggaaa aagggctgtt
     2641 ggaaatgtgg aaaggaagga caccaaatga aagattgtac tgagagacag gctaattttt
     2701 tagggaagat ctggccttcc cacaagggaa ggccagggaa ttttcttcag agcagaccag
     2761 agccaacagc cccaccagaa gagagcttca ggtttgggga agagacaaca actccctctc
     2821 agaagcagga gccgatagac aaggaactgt atcctttagc ttccctcaga tcactctttg
     2881 gcagcgaccc ctcgtcacaa taaagatagg ggggcaatta aaggaagctc tattagatac
     2941 aggagcagat gatacagtat tagaagaaat gaatttgcca ggaagatgga aaccaaaaat
     3001 gataggggga attggaggtt ttatcaaagt aagacagtat gatcagatac tcatagaaat
     3061 ctgcggacat aaagctatag gtacagtatt agtaggacct acacctgtca acataattgg
     3121 aagaaatctg ttgactcaga ttggctgcac tttaaatttt cccattagtc ctattgagac
     3181 tgtaccagta aaattaaagc caggaatgga tggcccaaaa gttaaacaat ggccattgac
     3241 agaagaaaaa ataaaagcat tagtagaaat ttgtacagaa atggaaaagg aaggaaaaat
     3301 ttcaaaaatt gggcctgaaa atccatacaa tactccagta tttgccataa agaaaaaaga
     3361 cagtactaaa tggagaaaat tagtagattt cagagaactt aataagagaa ctcaagattt
     3421 ctgggaagtt caattaggaa taccacatcc tgcagggtta aaacagaaaa aatcagtaac
     3481 agtactggat gtgggcgatg catatttttc agttccctta gataaagact tcaggaagta
     3541 tactgcattt accataccta gtataaacaa tgagacacca gggattagat atcagtacaa
     3601 tgtgcttcca cagggatgga aaggatcacc agcaatattc cagtgtagca tgacaaaaat
     3661 cttagagcct tttagaaaac aaaatccaga catagtcatc tatcaataca tggatgattt
     3721 gtatgtagga tctgacttag aaatagggca gcatagaaca aaaatagagg aactgagaca
     3781 acatctgttg aggtggggat ttaccacacc agacaaaaaa catcagaaag aacctccatt
     3841 cctttggatg ggttatgaac tccatcctga taaatggaca gtacagccta tagtgctgcc
     3901 agaaaaggac agctggactg tcaatgacat acagaaatta gtgggaaaat tgaattgggc
     3961 aagtcagatt tatgcaggga ttaaagtaag gcaattatgt aaacttctta ggggaaccaa
     4021 agcactaaca gaagtagtac cactaacaga agaagcagag ctagaactgg cagaaaacag
     4081 ggagattcta aaagaaccgg tacatggagt gtattatgac ccatcaaaag acttaatagc
     4141 agaaatacag aagcaggggc aaggccaatg gacatatcaa atttatcaag agccatttaa
     4201 aaatctgaaa acaggaaagt atgcaagaat gaagggtgcc cacactaatg atgtgaaaca
     4261 attaacagag gcagtacaaa aaatagccac agaaagcata gtaatatggg gaaagactcc
     4321 taaatttaaa ttacccatac aaaaggaaac atgggaagca tggtggacag agtattggca
     4381 agccacctgg attcctgagt gggagtttgt caatacccct cccttagtga agttatggta
     4441 ccagttagag aaagaaccca taataggagc agaaactttc tatgtagatg gggcagccaa
     4501 tagggaaact aaattaggaa aagcaggata tgtaactgac agaggaagac aaaaagttgt
     4561 ccccctaacg gacacaacaa atcagaagac tgagttacaa gcaattcatc tagctttgca
     4621 ggattcggga ttagaagtaa acatagtgac agactcacaa tatgcattgg gaatcattca
     4681 agcacaacca gataagagtg aatcagagtt agtcagtcaa ataatagagc agttaataaa
     4741 aaaggaaaaa gtctacctgg catgggtacc agcacacaaa ggaattggag gaaatgaaca
     4801 agtagataaa ttggtcagtg ctggaatcag gaaagtacta tttttagatg gaatagataa
     4861 ggcccaagaa gaacatgaga aatatcacag taattggaga gcaatggcta gtgattttaa
     4921 cctaccacct gtagtagcaa aagaaatagt agccagctgt gataaatgtc agctaaaagg
     4981 ggaagccatg catggacaag tagactgtag cccaggaata tggcagctag attgtacaca
     5041 tttagaagga aaagttatct tggtagcagt tcatgtagcc agtggatata tagaagcaga
     5101 agtaattcca gcagagacag ggcaagaaac agcatacttc ctcttaaaat tagcaggaag
     5161 atggccagta aaaacagtac atacagacaa tggcagcaat ttcaccagta ctacagttaa
     5221 ggccgcctgt tggtgggcgg ggatcaagca ggaatttggc attccctaca atccccaaag
     5281 tcaaggagta atagaatcta tgaataaaga attaaagaaa attataggac aggtaagaga
     5341 tcaggctgaa catcttaaga cagcagtaca aatggcagta ttcatccaca attttaaaag
     5401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     5461 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     5521 cagggacagc agagatccag tttggaaagg accagcaaag ctcctctgga aaggtgaagg
     5581 ggcagtagta atacaagata atagtgacat aaaagtagtg ccaagaagaa aagcaaagat
     5641 catcagggat tatggaaaac agatggcagg tgatgattgt gtggcaagta gacaggatga
     5701 ggattaacac atggaattct gcaacaactg ctgtttatcc atttcagaat tgggtgtcga
     5761 catagcagaa taggcgttac tcgacagagg agagcaagaa atggagccag tagatcctag
     5821 actagagccc tggaagcatc caggaagtca gcctaaaact gcttgtacca attgctattg
     5881 taaaaagtgt tgctttcatt gccaagtttg tttcatgaca aaagccttag gcatctccta
     5941 tggcaggaag aagcggagac agcgacgaag agctcatcag aacagtcaga ctcatcaagc
     6001 ttctctatca aagcagtaag tagtacatgt aatgcaacct ataatagtag caatagtagc
     6061 attagtagta gcaataataa tagcaatagt tgtgtggtcc atagtaatca tagaatatag
     6121 gaaaatggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6181 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6241 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6301 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6361 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6421 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     6481 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     6541 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     6601 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     6661 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     6721 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     6781 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     6841 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     6901 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     6961 gagacagatc cattcgatta gtgaacggat ccttggcact tatctgggac gatctgcgga
     7021 gcctgtgcct cttcagctac caccgcttga gagacttact cttgattgta acgaggattg
     7081 tggaacttct gggacgcagg gggtgggaag ccctcaaata ttggtggaat ctcctacaat
     7141 attggagtca ggagctaaag aatagtgctg ttagcttgct caatgccaca gccatagcag
     7201 tagctgaggg gacagatagg gttatagaag tagtacaagg agcttgtaga gctattcgcc
     7261 acatacctag aagaataaga cagggcttgg aaaggatttt gctataagct cgaggccgcc
     7321 ccggtgacct tcagaccttg gcactggagg tggcccggca gaagcgcggc atcgtggatc
     7381 agtgctgcac cagcatctgc tctctctacc aactggagaa ctactgcaac taggcccacc
     7441 actaccctgt ccacccctct gcaatgaata aaacctttga aagagcacta caagttgtgt
     7501 gtacatgcgt gcatgtgcat atgtggtgcg gggggaacat gagtggggct ggctggagtg
     7561 gcgatgataa gctgtcaaac atgagaatta attcttgaag acgaaagggc ctcgtgatac
     7621 gcctattttt ataggttaat gtcatgataa taatggtttc ttagtctaga attaattccg
     7681 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     7741 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     7801 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     7861 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     7921 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     7981 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     8041 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     8101 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     8161 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     8221 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     8401 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     8461 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8821 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg
//
