LOCUS       sequence-258740-       11093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MLV packaging plasmid for N-tropic MLV gag-pol expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C,
            Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J
  TITLE     Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 
            10.1038/s41564-019-0592-5. Epub 2019 Oct 21.
  PUBMED    31636416
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0592-5"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2044-2051"
COMMENT     Sequence Label: pCIG3 N
FEATURES             Location/Qualifiers
     source          1..11093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     589..613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          753..885
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     868..887
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        930..948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    5837..6211
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     6149..6165
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     polyA_signal    7063..7197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7100..7119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7154..7173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8001..8020)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8611..8630
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8805..8853
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8894..8913)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9013..9035
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9073..9091)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9159..9263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9264..9332,9333..10124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9482..9501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10295..10883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10784..10803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
      661 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc
      721 cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
      781 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
      841 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
      901 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agcctcgaga
      961 attcgagctc gcccgccgaa accgcgccgc gcgtcttgtc tgtctcagta ttgttttgtt
     1021 atttgtctgt tcgttattgt tttggaccgc ttctaaaaac atgggacaaa ccgtaaccac
     1081 ccctctgagt ctgaccctag aacactggaa agatgtccag cgcatcgcgt ccaatcagtc
     1141 cgtagatgtc aagaagagac gctgggtcac cttctgctct gccgagtggc caactttcgg
     1201 tgtagggtgg ccacaagatg gtacttttaa cttggatatt attctacagg ttaaatctaa
     1261 ggttttctct cctggtcccc acggacaccc ggatcaggtc ccatatattg tcacctggga
     1321 ggctattgcc tatgaacccc ctccgtgggt aaaacctttt gtctctccca aactctcccc
     1381 ctctccaacc gctcccatcc ttccatccgg tccttcgacc caacctccgc cccgatctgc
     1441 cctttaccct gctcttactc cctctataaa acccagacct tctaaacctc aggttctctc
     1501 cgataatggc ggacctctca ttgaccttct ctcagaagac cctccgccgt acggaggaca
     1561 gggaccgtcc tcctctgacg gagatggcga cagagaagag gccacctcca cttctgagat
     1621 tcctgccccc tctcccatag tgtctcgcct gcggggcaaa agagaccccc ccgcggcaga
     1681 ttccaccacc tctcgggctt tcccactccg tttggggggt aatggtcagt tgcagtactg
     1741 gccgttttcc tcctctgatc tatataactg gaaaaataat aatccttcct tctctgagga
     1801 tccaggtaaa ctgactgcat tgattgaatc tgtcctcacc acccaccagc ccacctggga
     1861 tgattgccag caattattag ggactctgct taccggggaa gagaagcagc gggtgctcct
     1921 ggaagcccga aaagctgtcc ggggcaacga tgggcgcccc acccaactgc ccaacgaggt
     1981 tgacgctgct tttccccttg aacgtcccga ttgggattac accacccaaa gaggtaggaa
     2041 ccacctagtt ctctatcgcc agttgctttt agcgggtctc caaaacgcgg gccgaagccc
     2101 caccaatttg gccaaggtaa aaggaataac ccagggacct gatgagtccc cctcagcctt
     2161 cctagagaga ctcaaggaag cctatcgcag atacactcct tatgatcctg aggaccctgg
     2221 gcaagaaacg aatgtatcta tgtcattcat ctggcagtcc gctccagaca ttggtcgaaa
     2281 gttagagcgg ttagaagact taaaaagtaa aactttagga gatttagtga gagaagccga
     2341 aaggatcttt aataaaagag aaactccaga agaaagagaa gagcgcgtta ggagggagac
     2401 agaggaaaag gaagagcggc gtagggcaga agaggagcag aaagagaaag agagggaccg
     2461 taggagacat agagaaatga gcaaactttt ggccaccgta gttagtggac agagacagga
     2521 tagacagggg ggagagcgaa ggaggcccca acttgacaag gatcaatgtg cctactgtaa
     2581 agaaaaagga cactgggcta aagactgccc aaagaagcca cggggtcccc gaggaccgcg
     2641 accccagacc tccctcctga ctttagacga ctaggggggt cagggtcagg agcccccccc
     2701 tgaacccagg ataaccctca ctgtcggggg gcaacccgtc accttcctgg tggatactgg
     2761 ggcccaacac tccgtgctga ctcaaaatcc tgggccccta agtgacaggt cggcttgggt
     2821 ccaaggggct actggaggaa agcggtatcg ctggaccaca gatcgcaagg tacacctagc
     2881 taccggtaag gtcactcact ctttcctcca tgtgccagac tgcccctacc ccttgctagg
     2941 aagagacttg ttaactaaac taaaagccca gatccacttt gagggatcag gagcccaggt
     3001 tgtgggacca aaaggacagc ctctacaggt gttgacccta aacctagaag atgagtatcg
     3061 gcttcatgag acctcagcag agccggaagc ttctcctggg tccacctggc tttccgactt
     3121 tccccaggcc tgggcagaaa ccgggggcat gggactggca gttcgccagg cgcctctaat
     3181 catacctctg aaggcaacct ccacccctgt gtccataaaa caatacccca tgtcacagga
     3241 agccagactg gggatcaagc cccacataca gaggctgttg gaccagggaa tactggtacc
     3301 ctgccagtcc ccctggaata cacccctgct acccgttaag aaaccaggaa ctaacgatta
     3361 taggcctgtc caggatctga gagaagtcaa caagcgggtg gaagacatcc accccaccgt
     3421 gcccaatcct tataacctcc taagtgggct cccaccgtcc caccggtggt atactgtgct
     3481 tgatttaaag gatgcctttt tctgcctgag actccacccc accagtcagc ctctcttcgc
     3541 cttcgagtgg agagatccag ggatgggaat ctcaggacaa ttaacctgga ccagactccc
     3601 acagggtttc aaaaacagtc ccaccctgtt tgatgaggca ctacacagag acctagcaga
     3661 cttccggatc cagcacccag acttgatcct gctacagtac gtggatgaca tactactggc
     3721 cgccacttct gagctcgact gccaacaagg tactcgggcc ctgttactaa ccctaggaaa
     3781 cctcgggtat cgggcctcgg ccaagaaagc ccaactttgc cagaaacagg tcaagtacct
     3841 ggggtatctc ctaaaagagg gtcagagatg gctgactgag gccagaaaag agactgtgat
     3901 ggggcagcct actccgaaga cccctcgaca actaagggag ttcctaggga cggcaggctt
     3961 ctgtcgcctc tggatccctg ggtttgcgga aatggcagcc cccttgtatc ctcttaccaa
     4021 aacggggact ctgttcaatt ggggcccaga ccagcaaaag gcctatcaag aaatcaaaca
     4081 ggccctccta actgcccccg ccctgggatt gccagatttg actaagccct ttgaactctt
     4141 tgtcgacgag aagcagggct acgccaaagg tgtcctaacg caaaaactgg gaccttggcg
     4201 tcggccggtg gcctacctgt ccaaaaagct agacccagta gcagctgggt ggcccccttg
     4261 cctacggatg gtagcagcca ttgccgtact gacaaaggat gcaggcaagc taaccatggg
     4321 acagccacta gtcattctgg ccccccatgc agtagaggca ctagtcaaac aaccccccga
     4381 ccgctggctt tccaacgccc ggatgactca ctatcaggcc ttgcttttgg acacggaccg
     4441 ggtccagttc ggaccggtgg tagccctgaa cccggctacg ctgctcccac tgcctgagga
     4501 agggctgcaa cacaactgcc ttgatatcct ggccgaagcc cacggaaccc gacccgacct
     4561 aacggaccag ccgctcccag acgccgacca cacctggtac acggatggaa gcagtctctt
     4621 acaagaggga cagcgtaagg cgggagctgc ggtgaccacc gagaccgagg taatctgggc
     4681 taaagccctg ccagccggga catccgctca gcgggctgaa ctgatagcac tcacccaggc
     4741 cctaaagatg gcagaaggta agaagctaaa tgtttatact gatagccgtt atgcttttgc
     4801 tactgcccat atccatggag aaatatacag aaggcgtggg ttgctcacat cagaaggcaa
     4861 agagatcaaa aataaagacg agatcttggc cctactaaaa gccctctttc tgcccaaaag
     4921 acttagcata atccattgtc caggacatca aaagggacac agcgccgagg ctagaggcaa
     4981 ccggatggct gaccaagcgg cccgaaaggc agccatcaca gagactccag acacctctac
     5041 cctcctcata gaaaattcat caccctacac ctcagaacat tttcattaca cagtgactga
     5101 tataaaggac ctaaccaagt tgggggccat ttatgataaa acaaagaagt attgggtcta
     5161 ccaaggaaaa cctgtgatgc ctgaccagtt tacttttgaa ttattagact ttcttcatca
     5221 gctgactcac ctcagcttct caaaaatgaa ggctctccta gagagaagcc acagtcccta
     5281 ctacatgctg aaccgggatc gaacactcaa aaatatcact gagacctgca aagcttgtgc
     5341 acaagtcaac gccagcaagt ctgccgttaa acagggaact agggtccgcg ggcatcggcc
     5401 cggcactcat tgggagatcg atttcaccga gataaagccc ggattgtatg gctataaata
     5461 tcttctagtt tttatagata ccttttctgg ctggatagaa gccttcccaa ccaagaaaga
     5521 aaccgccaag gtcgtaacca agaagctact agaggagatc ttccccaggt tcggcatgcc
     5581 tcaggtattg ggaactgaca atgggcctgc cttcgtctcc aaggtgagtc agacagtggc
     5641 cgatctgttg gggattgatt ggaaattaca ttgtgcatac agaccccaaa gctcaggcca
     5701 ggtagaaaga atgaatagaa ccatcaagga gactttaact aaattaacgc ttgcaactgg
     5761 ctctagagac tgggtgctcc tactcccctt agccctgtac cgagcccgca acacgccggg
     5821 cccccatggc ctcaccccat atgagatctt atatggggca cccccgcccc ttgtaaactt
     5881 ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc
     5941 tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact
     6001 ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca
     6061 ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc
     6121 caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc
     6181 cgaccccggg ggtggaccat cctctagact gacatggcgc gttcaacgct ctcaaaaccc
     6241 cttaaaaata aggttaaccc gcgaggcccc ctaatcccct taattcttct gatgctcaga
     6301 ggggtcagta ctgcttcgcc cggctccagt gcggcccagc cggccaccat gaaaacattt
     6361 aacatttctc aacaagatct agaattagta gaagtagcga cagagaagat tacaatgctt
     6421 tatgaggata ataaacatca tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt
     6481 tcggcagtac atattgaagc gtatatagga cgagtaactg tttgtgcaga agccattgcg
     6541 attggtagtg cagtttcgaa tggacaaaag gattttgaca cgattgtagc tgttagacac
     6601 ccttattctg acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg
     6661 gagttgattt cagactatgc accagattgt tttgtgttaa tagaaatgaa tggcaagtta
     6721 gtcaaaacta cgattgaaga actcattcca ctcaaatata cccgaaatta aaagttttac
     6781 caccaagctt atcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
     6841 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
     6901 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     6961 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
     7021 aagttcagat caaggtcagg aacagatgga acagggtcga ggaacttgtt tattgcagct
     7081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc
     7201 aggaagctcc tctgtgtcct cataaaccct aacctcctct acttgagagg acattccaat
     7261 cataggctgc ccatccaccc tctgtgtcct cctgttaatt aggtcactta acaaaaagga
     7321 aattgggtag gggtttttca cagaccgctt tctaagggta attttaaaat atctgggaag
     7381 tcccttccac tgctgtgttc cagaagtgtt ggtaaacagc ccacaaatgt caacagcaga
     7441 aacatacaag ctgtcagctt tgcacaaggg cccaacaccc tgctcatcaa gaagcactgt
     7501 ggttgctgtg ttagtaatgt gcaaaacagg aggcacattt tccccacctg tgtaggttcc
     7561 aaaatatcta gtgttttcat ttttacttgg atcaggaacc cagcactcca ctggataagc
     7621 attatcctta tccaaaacag ccttgtggtc agtgttcatc tgctgactgt caactgtagc
     7681 attttttggg gttacagttt gagcaggata tttggtcctg tagtttgcta acacaccctg
     7741 cagctccaaa ggttccccac caacagcaaa aaaatgaaaa tttgaccctt gaatgggttt
     7801 tccagcacca ttttcatgag ttttttgtgt ccctgaatgc aagtttaaca tagcagttac
     7861 cccaataacc tcagttttaa cagtaacagc ttcccacatc aaaatatttc cacaggttaa
     7921 gtcctcattt aaattaggca aaggaattcc tgcagcccgg gggatccgcc cgggctagag
     7981 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     8041 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     8101 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     8161 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     8221 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     8281 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8341 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8401 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8461 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8521 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8581 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8641 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8701 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     8761 aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat
     8821 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atccgcgtat
     8881 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     8941 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     9001 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     9061 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     9121 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     9181 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9241 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9301 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9361 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9421 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9481 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9541 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9601 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9661 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9721 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9781 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9841 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9901 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9961 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10021 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10081 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    10141 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10201 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10441 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10921 tttgctggcc ttttgctcac atggctcgac agatcttcaa tattggccat tagccatatt
    10981 attcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
    11041 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gtt
//
