LOCUS       sequence-25876-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Puro DEST (w16-1)
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      61..196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    236..357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(247..266)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     301..320
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             429..609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    684..808
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(833..838,839..856,857..863)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 gggggnnggg ccttcaggcc gcgtancgac tcactatagg gcgaattggg tacgtcccta
       61 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
      121 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
      181 gagttagggg cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga
      241 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      301 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttcct
      361 ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga
      421 actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
      481 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
      541 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
      601 gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgag ctagtccagt
      661 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
      721 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      781 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
      841 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
      901 nagctaagga agctaaaatg gagaaaaaaa
//
