LOCUS       sequence-258771-       10286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation lentiviral vector for hUbC-driven H2B-mCherry 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10286)
  TITLE     Lentiviral expression vector for H2B-mCherry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FUGW-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..10286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(81..101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(499..510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(619..635)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(620..636)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1141..1321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1347..1364)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1353..1577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1623..2051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1710..1729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1920..1941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2060..2080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2065..2394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2245..2380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2307..2326
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2442..2489
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2508..2882
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3012..3133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3049..3068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3103..3122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3182..3198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3182..3198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3195..3217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3206..3222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3230..3236,3237..3254,3255..3260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3275..3296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3413..3430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3584..4172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3664..3683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4343..5134,5135..5203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4966..4985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5204..5308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5383..5402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5574..5953
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5955..6153
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6108..6128
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6171..6351
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6398..6523
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7016..7249
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7434..7478
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7776..7893
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7958..9169
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9006..9025
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             9197..9574
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 ctagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
       61 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      121 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      181 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
      241 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
      301 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
      361 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
      421 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
      481 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
      541 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
      601 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
      661 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
      721 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
      781 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
      841 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
      901 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
      961 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     1021 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     1081 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     1141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     1201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     1261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1321 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     1381 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     1441 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     1501 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     1561 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     1621 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1681 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1741 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1801 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1861 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1921 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1981 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2041 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2101 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2161 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2221 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2281 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     2341 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2401 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     2461 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     2521 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     2581 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     2641 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     2701 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     2761 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     2821 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     2881 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     2941 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     3001 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3061 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3121 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     3181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3241 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3301 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3361 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3421 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3481 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3541 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3601 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3661 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3721 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3781 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3841 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3901 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3961 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4021 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4081 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4141 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4201 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4261 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4321 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4381 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4441 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4501 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4561 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4621 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4681 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4741 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4801 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4861 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4921 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4981 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5041 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5101 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5161 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5221 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5281 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     5341 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     5401 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     5461 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     5521 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     5581 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     5641 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     5701 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     5761 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5821 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     5881 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     5941 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     6001 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     6061 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     6121 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     6181 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6241 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6301 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6361 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6421 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6481 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6541 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6601 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6661 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6721 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6781 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6841 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     6901 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     6961 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7021 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7081 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7141 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7201 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7261 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7321 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7381 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7441 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7501 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7561 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7621 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     7681 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     7741 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7801 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7861 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     7921 ccagtttggt taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg
     7981 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     8041 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     8101 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     8161 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     8221 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     8281 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     8341 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     8401 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     8461 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     8521 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     8581 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     8641 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     8701 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     8761 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     8821 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     8881 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     8941 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     9001 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     9061 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     9121 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc
     9181 tgcaggtcga ctctagatgc cagagccagc gaagtctgct cccgccccga aaaagggctc
     9241 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
     9301 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
     9361 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
     9421 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
     9481 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
     9541 tactaaggcc atcaccaagt acaccagcgc taagatggtg tctaaaggcg aggaggataa
     9601 catggctata atcaaggaat ttatgaggtt caaggtgcat atggagggat ctgtgaacgg
     9661 tcacgagttc gaaatcgagg gcgaaggcga ggggcgaccc tatgaaggga cacagacggc
     9721 caaactgaag gtgaccaagg gtggccccct gcctttcgcc tgggacatcc tgtcacccca
     9781 gtttatgtac ggctctaagg cttacgttaa gcaccctgcc gatattccgg actacttgaa
     9841 actgagcttc cctgaagggt tcaagtggga acgggtaatg aattttgagg atggcggtgt
     9901 agtcacagtt acccaggaca gctctcttca ggacggagaa tttatctata aggttaaact
     9961 tcggggcact aacttcccat cagacggccc cgtgatgcag aagaaaacta tgggctggga
    10021 agccagctca gaacgcatgt atcccgagga cggagcactg aagggagaaa tcaagcagcg
    10081 actcaagctg aaggatgggg gacattatga tgctgaggtc aaaaccacct ataaggccaa
    10141 gaaacccgtt cagcttcctg gggcatacaa cgtgaatatc aaactggata ttacatccca
    10201 taacgaagac tataccatcg tggaacagta cgagcgggcc gagggcagac atagcacagg
    10261 gggcatggat gaattgtaca agtaat
//
