LOCUS       sequence-258780-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0629
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             201..1694
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             complement(join(2220..3011,3012..3080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2843..2862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3081..3185)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3288..3876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3777..3796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4894..4914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccatatccg catccatacc ggtcagaagc ccttccagtg tcgaatctgc atgcgtaact
       61 tcagtcgtag tgaccacctt accacccaca tccgcaccca cacaggcgag aagccttttg
      121 cctgtgacat ttgtgggagg aagtttgcca ggagtgatga acgcaagagg cataccaaaa
      181 tccatacagg tgctagcttc atgaagatgg acaaaaagac tatagtttgg tttagaagag
      241 atctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga tctgtttttc
      301 ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga gcttcaagat
      361 ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt ggatctgacc
      421 tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc cgcgttaccg
      481 gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt cgggaccata
      541 ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat ggagatctat
      601 tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt ttcaattctt
      661 actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct ccttggcggt
      721 tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa gaactagggc
      781 tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg agtccaggat
      841 ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata gattatgcaa
      901 agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat ctccatttcg
      961 gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt atatgggcaa
     1021 gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga atcggtttaa
     1081 gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa tcgttgttga
     1141 gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc tggagacaag
     1201 gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct accggatgga
     1261 tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt ctccttccat
     1321 ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg gaatgtgaca
     1381 tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt gatcgcttgg
     1441 acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata aggcaatggc
     1501 ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac gctcctttaa
     1561 ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc attgtagaca
     1621 tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa gcacagatca
     1681 tgatcggagc agcagcccgg ggatcctaac tcgaggccaa ttggtgcggc aattgataat
     1741 aacgaaaatg tcttttaatg atctgggtat aatgaggaat tttccgaacg tttttacttt
     1801 atatatatat atacatgtaa catatattct atacgctata gagaaaggaa atttttcaat
     1861 taaaaaaaaa atagagaaag agtttcactt cttgattatc gctaacacta atggttgaag
     1921 tactgctact ttaattttat gctggatgtg agacgataaa tcgcagccaa gtgagtgaat
     1981 agatgacgca ccacggtcag acacggccac atcgtatctc acaggagcaa gcgcgatagg
     2041 agcactcaca catagtacgg tgatccgctg actcctttgc ccaaataaga cgtgagccac
     2101 tagtgcactg cagtacagcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta
     2161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2341 agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac
     2401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3181 ccgcgcacat ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt
     3241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3961 tctgtggata accgtgcggc cgcccctgaa ttcgcatcta gaacggggtc atcacggctc
     4021 atcatgcgcc aaacaaatgt gtgcaataca cgctcggatg actgcatgat gaccgcactg
     4081 actggggaca gcagatccac ctaagcctgt gagagaagca gacacccgac agatcaaggc
     4141 agttacgtct caccaaacca gatgtcaaca cagctacaac gagtcgaaca agaagcaggc
     4201 aaagtttaga gcactgcccc tccgcactca aaaaagaaaa aactaggagg aaaataaaat
     4261 tctcaaccac acaaacacat aaacacatac aaatacaaat acaagcttat ttacttgaca
     4321 tcgcgcgatc ttccactatt cagcgccgtc cgccctctct cgtgtttttt gtttacgcga
     4381 caactatgcg aaatccggag caacgggcaa ccgtttgggg aaagaccaca cccacgcgcg
     4441 atcgccatgg caacgaggtc gcacacgccc cacacccaga cctccctgcg agcgggcatg
     4501 ggtacaatgt ccccgttgcc acagacacca cttcgtagca cagcgcagag cgtagcgtgt
     4561 tgttgctgct gacaaaagaa aatttttctt agcaaagcaa aggaggggaa gcacgggcag
     4621 atagcaccgt accataccct tggaaactcg aaatgaacga agcaggaaat gagagaatga
     4681 gagttttgta ggtatatata gcggtagtgt ttgcgcgtta ccatcatctt ctggatctat
     4741 ctattgttct tttcctcatc actttcccct ttttcgctct tcttcttgtc ttttatttct
     4801 ttcttttttt taattgttcc ctcgattggc tatctaccaa agaatccaaa cttaatacac
     4861 gtatttattt gtccaattac cagatctatg gcccccaaga agaagaggaa ggttggtatt
     4921 cacgggggta cccgcccata tgcttgccct gtcgagtcct gcgatcgccg cttttctcgc
     4981 tcggatgagc ttaccc
//
