LOCUS       sequence-258781-       11420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, 
            Stewart R, Thomson JA
  TITLE     An In Vitro Human Segmentation Clock Model Derived from Embryonic 
            Stem Cells.
  JOURNAL   Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 
            10.1016/j.celrep.2019.07.090.
  PUBMED    31461642
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-27- 27"; volume: "28"; issue: "9"; pages: "2247-2255.e5"
COMMENT     Sequence Label: pB-TAG-HES7
FEATURES             Location/Qualifiers
     source          1..11420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1139..1157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1176..1194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1213..1231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1250..1268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1287..1305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1324..1342
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1361..1379
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1398..1416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1438..1462
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1542..1566
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2293..2317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(2359..2933,2934..2936,2937..2945)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             join(2946..2948,2949..2951,2952..3665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2946..2948,2949..2951,2952..3662)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2991..3012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3252..3271)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3599..3620
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3677..3696)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3742..3797
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3796..3815)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        4240..4619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4734..6039
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    5076..6007
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(6056..6673,6674..6679,6680..6715,6716..6718,6719..6754,
                     6755..6757,6758..6793,6794..6802)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(6829..7403,7404..7406,7407..7415)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7416..8015
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7416..7435)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7912..7932
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8101..8673
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   9047..9081
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9230..9248)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9269..9285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9269..9285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9282..9304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9293..9309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9317..9323,9324..9341,9342..9347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9362..9383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9500..9517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9671..10259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9751..9770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10430..11221,11222..11290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11053..11072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11291..11395)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 acaaaaactc aaaatttctt ctataaagta acaaaacttt taaacattct ctcttttaca
      961 aaaataaact tattttgtac tttaaaaaca gtcatgttgt attataaaat aagtaattag
     1021 cttaacctat acataataga aacaaattat acttattagt cagtcagaaa caactttggc
     1081 acatatcaat attatgctct cgttaattaa ctcgagggat ccaagctggc cgcacgtctc
     1141 cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata gagaaggtac
     1201 gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct ccctatcagt
     1261 gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg acacgtctcg
     1321 agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag tgatagagaa
     1381 gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt accccctata taagcagagc
     1441 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     1501 aagacaccgg gaccgatcca gcctccgcgg tggcggccat cacaagtttg tacaaaaaag
     1561 caggcttgga aggagttcga accatggtca cccgggatcg agctgagaat agggacggcc
     1621 ccaagatgct caagccgctt gtggagaagc ggcgccggga ccgcatcaac cgcagcctgg
     1681 aagagctgag gctgctgctg ctggagcgga cccgggacca gaacctccgg aacccgaagc
     1741 tggagaaagc ggagatattg gagttcgccg tgggctactt gagggagcga agccgggtgg
     1801 agcccccggc cgccgcggct ccaggggttc cccggtcccc agtccaggac gccgaggcgc
     1861 tcgccagctg ctacttgtcc ggtttccgcg agtgcctgct tcgcttggcg gccttcgcgc
     1921 acgacgccag cccggccgcc cgcgcccagc tcttctccgc gctgcacggc tatctgcgcc
     1981 ccaaaccgcc ccggcccaag ccggtagatc cgaggcctcc agcgccgcgc ccatccctgg
     2041 accccgccgc accggccctt ggccctgcgc tgcaccagcg ccccccagtg caccagggcc
     2101 accctagccc gcgctgcgca tggtccccat ccctctgctc cccgcgcgcc ggggattctg
     2161 gcgcgccggc gcccctcacc ggactgctgc cgccgccacc gccgcctcac agacaagacg
     2221 gggcgcccaa ggccccgctg cccccgccgc ccgctttctg gagaccttgg ccctagctcg
     2281 agtgcggccg caacccagct ttcttgtaca aagtggtgat ctagctaaaa cgcggcctcg
     2341 aatcgaattc cgcccccccc cctctccctc ccccccccct aacgttactg gccgaagccg
     2401 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2461 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2521 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2581 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     2641 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     2701 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     2761 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     2821 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     2881 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     2941 caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3001 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3061 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3121 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3181 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3241 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3301 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3361 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3421 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3481 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3541 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3601 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3661 agtaaagcgg ccgcgatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3721 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3781 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3841 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3901 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3961 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     4021 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4081 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcaggaat
     4141 tcgattactt aagcctagga tccagatctg tacattcgaa tcgatgaatt cgttaaccat
     4201 ggaagcttct gcagcggccg cactagcggt ggcggcctcg acattgatta ttgactagtt
     4261 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4321 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     4381 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     4441 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     4501 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     4561 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatga
     4621 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     4681 caagtctcca ccccattgac gtcaatggga gtttgttttg actagtggag ccgagagtaa
     4741 ttcatacaaa aggagggatc gccttcgcaa ggggagagcc cagggaccgt ccctaaattc
     4801 tcacagaccc aaatccctgt agccgcccca cgacagcgcg aggagcatgc gcccagggct
     4861 gagcgcgggt agatcagagc acacaagctc acagtccccg gcggtggggg gaggggcgcg
     4921 ctgagcgggg gccagggagc tggcgcgggg caaactggga aagtggtgtc gtgtgctggc
     4981 tccgccctct tcccgagggt gggggagaac ggtatataag tgcggtagtc gccttggacg
     5041 ttctttttcg caacgggttt gccgtcagaa cgcaggtgag tggcgggtgt ggcttccgcg
     5101 ggccccggag ctggagccct gctctgagcg ggccgggctg atatgcgagt gtcgtccgca
     5161 gggtttagct gtgagcattc ccacttcgag tggcgggcgg tgcgggggtg agagtgcgag
     5221 gcctagcggc aaccccgtag cctcgcctcg tgtccggctt gaggcctagc gtggtgtccg
     5281 ccgccgcgtg ccactccggc cgcactatgc gttttttgtc cttgctgccc tcgattgcct
     5341 tccagcagca tgggctaaca aagggagggt gtggggctca ctcttaagga gcccatgaag
     5401 cttacgttgg ataggaatgg aagggcagga ggggcgactg gggcccgccc gccttcggag
     5461 cacatgtccg acgccacctg gatggggcga ggcctgtggc tttccgaagc aatcgggcgt
     5521 gagtttagcc tacctgggcc atgtggccct agcactgggc acggtctggc ctggcggtgc
     5581 cgcgttccct tgcctcccaa caagggtgag gccgtcccgc ccggcaccag ttgcttgcgc
     5641 ggaaagatgg ccgctcccgg ggccctgttg caaggagctc aaaatggagg acgcggcagc
     5701 ccggtggagc gggcgggtga gtcacccaca caaaggaaga gggccttgcc cctcgccggc
     5761 cgctgcttcc tgtgaccccg tggtctatcg gccgcatagt cacctcgggc ttctcttgag
     5821 caccgctcgt cgcggcgggg ggaggggatc taatggcgtt ggagtttgtt cacatttggt
     5881 gggtggagac tagtcaggcc agcctggcgc tggaagtcat tcttggaatt tgcccctttg
     5941 agtttggagc gaggctaatt ctcaagcctc ttagcggttc aaaggtattt tctaaacccg
     6001 tttccaggtg ttgtgaaagc caccgctaat tcaaagcaat ccggagtaat tcaccatgtc
     6061 tagactggac aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat
     6121 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     6181 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     6241 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     6301 cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     6361 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     6421 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     6481 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     6541 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     6601 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     6661 gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc
     6721 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     6781 ccttgacatg ctccccgggt aactaagtaa ggatcattcc gccccccccc cctctccctc
     6841 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     6901 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     6961 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     7021 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     7081 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     7141 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     7201 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     7261 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     7321 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7381 cctttgaaaa acacgatgat aatatggcca caaccatgac cgagtacaag cccacggtgc
     7441 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7501 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7561 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7621 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     7681 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     7741 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7801 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7861 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7921 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7981 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     8041 cgaccgaaag gagcgcacga ccccatgcat cgaaatgacc gaccaagcga attcgctagg
     8101 attatcccta atacctgcca ccccactctt aatcagtggt ggaagaacgg tctcagaact
     8161 gtttgtttca attggccatt taagtttagt agtaaaagac tggttaatga taacaatgca
     8221 tcgtaaaacc ttcagaagga aaggagaatg ttttgtggac cactttggtt ttcttttttg
     8281 cgtgtggcag ttttaagtta ttagttttta aaatcagtac tttttaatgg aaacaacttg
     8341 accaaaaatt tgtcacagaa ttttgagacc cattaaaaaa gttaaatgag aaacctgtgt
     8401 gttcctttgg tcaacaccga gacatttagg tgaaagacat ctaattctgg ttttacgaat
     8461 ctggaaactt cttgaaaatg taattcttga gttaacactt ctgggtggag aatagggttg
     8521 ttttcccccc acataattgg aaggggaagg aatatcattt aaagctatgg gagggttgct
     8581 ttgattacaa cactggagag aaatgcagca tgttgctgat tgcctgtcac taaaacaggc
     8641 caaaaactga gtccttgggt tgcatagaaa gctgccaaac gctagccaac aagctcgtca
     8701 tcgctttgca gaagagcaga gaggatatgc tcatcgtcta aagaactacc cattttatta
     8761 tatattagtc acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     8821 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     8881 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     8941 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     9001 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     9061 acgcagacta tctttctagg gttaaaaaag atttgcgctt tactcgacct aaactttaaa
     9121 cacgtcatag aatcttcgtt tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg
     9181 cgcgctaaag aatggcaaac caagtcgcgc gaggtaccag cttttgttcc ctttagtgag
     9241 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9481 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10081 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11401 ttccccgaaa agtgccacct
//
