LOCUS       sequence-258791-       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression 
            of isoform a of DAF-16 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH3185
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..68
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    185..206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(221..226,227..244,245..251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    259..275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     264..286
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3881..3909)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4657..4677
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8218..8235)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8218..8234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8227..8249)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8447..8902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8534..8553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8744..8765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8919..8938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9038..9060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9098..9116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9184..9288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9289..9357,9358..10149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9507..9526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10320..10908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10809..10828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
       61 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      121 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      181 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      241 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
      301 tgattacgaa ttcgaatggc catgggacgt cgacgcttgc atgcctgcag cgtttccgat
      361 acccccttat atcagcacac attcagtcat cataccacaa aatgttgtga tagtgatagg
      421 caaaactacc gtatttcctc tattaatctt gcatgcaaga ctaattttcg attaacccgt
      481 aggggtgcaa gactaataga gactgcaaga ctattagagg ctgaaatact aattttcgta
      541 tgctcaataa ttttggaaat tggcctattt tttgtagaaa cttgataccg tttaaacaag
      601 gaaaaataca cactttttaa tatttcatca ataatttgaa cgattttgtg attttaagtt
      661 caatttgccc aaaaaaagac aatttttctg acgttaccgc aaagtaatgc tggtcaggca
      721 aaaagagcgg tgcaaaaata taagagactg caatactaat agaggaaata cggtaattga
      781 attttagtga aacttgcgac agttttcctc atttttttgt tattccggca tacgagtgtg
      841 gcatacgagt gaggtcatct tttgtttctt ccgtttcttc atcgcttttg aaaaaaaatg
      901 ttgaagaaaa cttcgtagcc gatagccgga taatgtttgg cacggcgttc caacatatat
      961 cattggaaat tttttaagat ttttgcgaaa aatcacattc ttcacgatga gaacacgtta
     1021 ttgaaggaat ataaatcaag aataacatat agttatattt ctctattact ttaacgttaa
     1081 atatgagcaa atttgagcat tttgattgcg atgaaaagca gaatcgagtc aactgaaatc
     1141 cgttcaaaaa cctaagctcg gttcgcccga aagtcgattt ttccaatagc cgaacagcac
     1201 tgcttcttta ttttccaggt atttttgggg tgtaagaatt atatcttctt tgtttgggta
     1261 tttacgtttc cgaattccct ttcttgatct gtcgctattt ttcagaaacc agaacagttt
     1321 atcctttttc atgagaacct gaatagctca aaaacccgtt taatttcttt tcatcatctc
     1381 atttgaaact ttcctagtct tcttaatgat ttccaggctc ctcctacatt tgtatctcaa
     1441 gatgcataca ctttatttcc gctccaattc gaatcatcta cttctctttt ttcttcaact
     1501 ttctactttt ttccattctt ttctttcaaa tgttcgacgt cttcaagtga ctgctcttca
     1561 ttctcctcat ttttctcgaa tttcattctt gtctcttttt tgctcaaaaa tggaaaatga
     1621 aagtgacgtg agatttggac ggcgggacac gggggcagta gaagcagcaa aaaggagaga
     1681 aagaggacac aaataagaag aacgaattca aaaataagcg gagaggagct atttccgtca
     1741 attctacctc cccaatcttc atcaattcgg ctcaattgaa tgacgtcaca gggagataaa
     1801 ccgtttggat gagcgccgtc catcactgac gccatcccgt ttgggacaag aaaagagaaa
     1861 aaagagcaca aagtttttgg tgacggatct tgtcaatcat atgaaagttg ttctgattga
     1921 ttgtcagttt ttttcctact tttttggatt ctatccactt ctgaactttt gacaagtttc
     1981 aaactttctg aatcattttc tatgcatttt cctggaattc tttttatgta aaatatgaaa
     2041 tagaatgttt ttgaattcaa gttctgcttt tttcttcttt tctgttctgt tcgggcttgg
     2101 gtatgctttt tttaaaaatt attttgcaca tcgaccaata agtgcgcaac ttataaaatt
     2161 aatttatttt tgttaatttt tgaaatactt gtattgcttt aagtgatctg acctcgccct
     2221 gagctttcca cgtagttatc aaatacaaat cctccaaggg taacgtacct atattactga
     2281 tctttataat aactttatca cctgtccagt tccagaggaa ttctgttaag cttataggca
     2341 cagaaggagt catttctgct ggtttttaat gatccaaatc tttatttcaa gtaaaaaact
     2401 gaacacttgc gaataaaact atcagattaa ccattcacca aaaatgtgtt tgaatctaaa
     2461 actttctcag tattccaaat atagaaataa ataaccacga cattgctaaa atctgtctga
     2521 attgtgtact ccttaccgtg aagtaataat ggatataatg aatcgtttga aatgaatgat
     2581 cagcacattt ttggtgaaag atcacaaata aggaataagc gtcggaaaat aaaacgatta
     2641 tttcggatca aaaatttgtt gaagatcata tacactcgag accaagatta ttctagacaa
     2701 ttttcaaatt ggcttctttg tttgcaaatc ttcataataa tctgtgaagt ttggaaattt
     2761 gaatttttaa tcttttttca tagattatag tttttatttc tttgcaaaac tatattaaaa
     2821 accgatgcat tgttttaggg aaattaatga gccttttgtt caacactaaa aacaataaaa
     2881 ttaaaatttt ggcttcatca tttgaccttt tttaagttcg aaaacttttc tcgtatttct
     2941 gaaccgccaa ttttttcaca catctctaga cttttggtgc ccgttccaga aagttaagta
     3001 attgctattc taagaaagtt ctcaacattg ttttttagtt ctgattgaat tctgatgttc
     3061 caggaatata ttttaaattt aatattcttt gcactatttc tatactaact aaataataaa
     3121 tagtctaagt atgttcaatg agaacaaaaa agctctatct atatttttct atcctatttc
     3181 atttatatcc ttttcatttt gaactcacca gcttctcttc ttcttcttgt ccatatactt
     3241 cttaaaagtc tcgaacttct ctctctcact ctccatcaat ttctttatgg ctaccgcttg
     3301 cgctctgccg cttccgaaga aagaaggcgt tggcagagct ctcaattcta ttttttctcg
     3361 ccgtaggcta tcgatttctc agcttctctt ctctcttcct cactccacca ccaccacttc
     3421 gggcttcttc ttttcttcat tttcgtcttc ttcttcatcc tcttcttttt ttttcagttc
     3481 cccctcactc ctccccttat atgcgcgtgc gagaggggtg caaaagcagc gcgcatccga
     3541 gaattgaaac gagaaaacgg agacgcagca gcagttcgtc ctcagaaaaa tagccagtaa
     3601 aaagagaaaa agatagagag agacctatcg cattttattt tcaaattgca attcctgcaa
     3661 ttcatgtgtg cgtgtgccca ttttcaattc ttcccgttat ttttcagacc attaccaacg
     3721 ttctttctag atcttgagac aatcttcctt ctgctcaatc gttcgtcgtg aagacggcat
     3781 cgacgacgac gacgatcagc agtaaaggat ccccgggtac cgagctcgaa ttcaagtacc
     3841 ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     3901 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     3961 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     4021 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agccaacact
     4081 tgtcactact ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg
     4141 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     4201 caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta actaaccata
     4261 catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     4321 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     4381 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     4441 tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc agaacttcaa
     4501 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     4561 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     4621 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     4681 tgctgggatt acacatggca tggacgaact atacaaaagc ccacaagctt acatgtcctg
     4741 ggcccatcga ttcggacgtc ccgagggctt aagcggacgc gtcgagggga tccctatgat
     4801 ggagatgctg gtagatcagg gaactgatgc atcgtcatcc gcctccacgt ccacctcatc
     4861 tgtttcgaga ttcggagcgg acacgttcat gaatacaccg gatgatgtga tgatgaatga
     4921 tgatatggaa ccgattcctc gtgatcggtg caatacgtgg ccaatgcgta ggccgcaact
     4981 cgaaccacca ctcaactcga gtcccattat tcatgaacaa attcctgaag aagatgctga
     5041 cctatacggg agcaatgagc aatgtggaca gctcggcgga gcatcttcaa acgggtcgac
     5101 agcaatgctt catactccag atggaagcaa ttctcatcag acatcgtttc cttcggaaat
     5161 gtccgaatcg ccagacgata ccgtatcggg aaaaaagaca acgaccagac ggaacgcttg
     5221 gggaaatatg tcatatgctg aacttatcac tacagccatt atggctagtc cagagaaacg
     5281 gttaactctt gcacaagttt acgaatggat ggtccagaat gttccatact tcagggataa
     5341 gggagattcg aacagttcag ctggatggaa gaactcgatc cgtcacaatc tgtctcttca
     5401 ttctcgtttc atgcgaattc agaatgaagg agccggaaag agctcgtggt gggttattaa
     5461 tccagatgca aagccaggaa ggaatccacg gcgtacacgt gaacgatcca atactattga
     5521 gacgactaca aaggctcaac tcgaaaaatc tcgccgcgga gccaagaaga ggataaagga
     5581 gagagcattg atgggctccc ttcactcgac acttaatgga aattcgattg ccggatcgat
     5641 tcaaacgatt tctcacgatt tgtatgatga tgattcaatg caaggagcat ttgataacgt
     5701 tccatcgtct ttccggttag taatttagtt ttatttttat tttttgaatt ttccaaattt
     5761 ccaaattttt tcagtccccg aactcaatcg aacctctcga ttcctggatc gtcgtctcgt
     5821 gtttctccag ctattggaag tgatatctat gatgatctag aattcccatc atcggttggc
     5881 gaatcggttc cagcaattcc aagtgatatt gttgatagaa ctgatcaaat gcgtatcgat
     5941 gcaactactc atattggtgg agttcagatt aagcaggagt cgaagccgat taaggtgaga
     6001 aatgagaatt tgcaattttc aggaaaattt gaaaattttc ttgtggttag tacaagtgag
     6061 ctccttttta gacaattaaa aaatttgaat tttacttaaa ttcaatattt ttaattctga
     6121 atttagtttc aaggtcccct aaaatattag taacatgcaa atcattttat attttctcac
     6181 accgaaaatt agaattttct ttttttttaa gtgaaaaaac tgagtaatat atagaatttt
     6241 cagacggaac caattgctcc accaccatca taccacgagt tgaacagtgt ccgtggatcg
     6301 tgtgctcaga atccacttct tcgaaatcca attgtgccaa gcactaactt caagccgtta
     6361 gttttttttt gaacagaagt taattaatct atcgtaattt cacaagattt tgggtcccgg
     6421 cgctcaaaaa tgtttttttt aaatttttat gttaaaaata gttttaaatc agttttgaaa
     6481 gtgtaaattt tggcttaaaa ttattttaaa cagaaaattg gaaataaaac gaagaaataa
     6541 acttttttgc gcgccgagac ccatctgaat aaatgcgtgc gcctttaaga aacctgtgaa
     6601 attactatat atacaaaatt ctgagaatgc gtattacgca acaaatttga cgcgcaaaat
     6661 atctcgtagc gaaaactaca gtaatcctta aaatgagtac tgtagcgctt gcgtcgattt
     6721 acgggctcga ttttcgtgaa ttaatttatt ttcgaaaagt gacagcgata ttacatttaa
     6781 ctttgttctt tctaatcatt ttctcatttt tgtgaatttt taaaaataat ttttcgatta
     6841 ataaaggatt gccgtaaatc gacacatggt gctacagtag gcatttaaag gattactgta
     6901 gttttcgcta cgagatattt cgcgcgtcaa atatgttgcg taatacgcat tctcagaatt
     6961 ttatgatccc ataatatata tttcttcttt aattcaatat ataataatta tccaaaattt
     7021 gttcagaatg ccactaccgg gtgcctatgg aaactatcaa aatggtggaa taactccaat
     7081 caattggcta tcaacatcca gctcatctcc actgcctgga attcaatcgt gtggaattgt
     7141 agctgcacag catactgtcg cttcttcatc ggctcttcca attgatttgg aaaatctgac
     7201 acttcccgat cagccactga tggatactat ggatgttgat gcattgatca gacatgagct
     7261 gagtcaagct ggagggcagc agcatattca ttttgatttg taactgcagg aattcgatat
     7321 gcagaagctg atctcagagg aggacctgct tcagaagctt atctcagagg aggaccttcg
     7381 acctcgaggg ggggcccggt accatggtat tgatatctga gctccgcatc ggccgctgtc
     7441 atcagatcgc catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc
     7501 cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc
     7561 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     7621 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     7681 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta
     7741 cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     7801 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     7861 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     7921 gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc
     7981 aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     8041 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     8101 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     8161 taccaaacat aactgtttct actagtggcc acgtgggccg tgcaccttaa gcttggcact
     8221 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8281 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     8341 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     8401 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     8461 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8521 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8581 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8641 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     8701 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8761 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     8821 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8881 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     8941 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9001 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9061 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     9121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     9181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10441 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10981 ttatcccctg attctgtgga taaccgtatt a
//
