LOCUS       sequence-25880-e         513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Gateway destination vector, Expression, CMV/TO promoter, 
            Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXP CMV/TO DEST (w360-1)
FEATURES             Location/Qualifiers
     source          1..513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..195
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(338..357)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tacccggtag aattccggat ccgttaatta agcgtacgag gcctaccggt cgccactgtg
       61 ctggatatca accactttgt acaagaaagc tgaacgagaa acgtaaaatg atataaatat
      121 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
      181 tatccagtca ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata
      241 ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag
      301 ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc
      361 agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca
      421 gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct
      481 gtacatccac aaacagacga taacggctct ctc
//
