LOCUS       sequence-25881-e         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for miRNA expression under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pSM2(U6) (w51-1)
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           96..189
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           203..315
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(347..446)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(558..577)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 atttcttggc tttntatatc ttgtggaaag gangaaacac cgtgctcgct tcggcagcac
       61 atatactagt cgactaggga taacagggta attgtttgaa tgaggcttca gtactttaca
      121 gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa
      181 cagaaggctc gagcaaccag aattcaaggg gctactttag gagcaattat cttgtttact
      241 aaaactgaat accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat
      301 aaattaaatc acttttttca attggaagac taatgcgttt atctagaccc agctttcttg
      361 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
      421 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa
      481 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
      541 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgt
//
