LOCUS       sequence-258821-       10429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for tagging human HES7 C-terminus with NanoLuc and 
            tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, 
            Stewart R, Thomson JA
  TITLE     An In Vitro Human Segmentation Clock Model Derived from Embryonic 
            Stem Cells.
  JOURNAL   Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 
            10.1016/j.celrep.2019.07.090.
  PUBMED    31461642
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-27- 27"; volume: "28"; issue: "9"; pages: "2247-2255.e5"
COMMENT     Sequence Label: HES7-NLuc-2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..10429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             905..1417
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             1427..1483
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      1499..1508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1505..2935
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    3057..3178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3094..3113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3148..3167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3190..3223)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        3242..3741
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3277..3300)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3674..3693
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        3758..3805
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3824..4627
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3887..3906)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4497..4516
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(4766..4799)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(5802..6390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5882..5901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6561..7352,7353..7421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7422..7526)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(7594..8928,8929..9015))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(9016..9461)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     protein_bind    9476..9509
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             9538..10197
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9604..9623)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      10218..10312
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10348..10383)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 gcatgcctgc aggtcgacgc gccctcccct cccgtctgta gaacctccgg aacccgaagc
       61 tggagaaagc ggagatattg gagttcgccg tgggctactt gagggagcga agccgggtgg
      121 agcccccggg tacagcgcgg gggtggggca gcggagggag ggagggggga cgccaggagg
      181 ctccggccgg ggcagagtgg gcgggccctg cggtggatgg tgggcttggg gagtggccgg
      241 ttctgctccg aggcgaggtt aataacaccc gcgcgtgtct gtctctgtgt ctccctcatt
      301 ttctccctct ttccgtccat ctggcttcct gtctctgtgc gcctgtccgg ccttggtatc
      361 tctgcgcccc gccccttccc tcacccctgc cgggcccctg ccctgcccac ctgtgctccc
      421 gccggccgca ccagccgccg cggctccagg ggttccccgg tccccagtcc aggacgccga
      481 ggcgctcgcc agctgctact tgtccggttt ccgcgagtgc ctgcttcgct tggcggcctt
      541 cgcgcacgac gccagcccgg ccgcccgcgc ccagctcttc tccgcgctgc acggctatct
      601 gcgccccaaa ccgccccggc ccaagccggt agatccgagg cctccagcgc cgcgcccatc
      661 cctggacccc gccgcaccgg cccttggccc tgcgctgcac cagcgccccc cagtgcacca
      721 gggccaccct agcccgcgct gcgcatggtc cccatccctc tgctccccgc gcgccgggga
      781 ttctggcgcg ccggcgcccc tcaccggact gctgccgccg ccaccgccgc ctcacagaca
      841 agacggggcg cccaaggccc cgctgccccc gccgcccgct ttctggagac cttggcccgg
      901 atccatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa
      961 cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc
     1021 cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca
     1081 tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt
     1141 taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact
     1201 ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat
     1261 cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat
     1321 tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg
     1381 agtgaccggc tggcggctgt gcgaacgcat tctggcggga agcggagcta ctaacttcag
     1441 cctgctgaag caggctggag acgtggagga gaaccctgga cctacccggg taccggtcgc
     1501 caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     1561 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1621 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     1681 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     1741 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1801 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     1861 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     1921 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     1981 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     2041 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     2101 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     2161 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     2221 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     2281 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2341 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     2401 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     2461 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2521 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     2581 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     2641 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     2701 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     2761 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     2821 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     2881 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaggcggc
     2941 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3001 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     3181 gcgagatctg aagttcctat actttctaga gaataggaac ttcacgaagt tatattctac
     3241 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     3301 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     3361 cgccaaccgg ctccgttctt tggtggcccc gtcgcgccac cttctactcc tcccctagtc
     3421 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     3481 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     3541 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     3601 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     3661 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     3721 tcctcatctc cgggcctttc gacctgcagc agcacgtgtt gacaattaat catcggcata
     3781 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgggat cggccattga
     3841 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3901 ctgggcacaa cagacgatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3961 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4021 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4081 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4141 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4201 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4261 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4321 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     4381 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4441 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4501 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4561 ttacggtatc gccgcccccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4621 cttctgagcg ggactctggg gttcgaataa agaccgacca agcgacgtct gagagctccc
     4681 tggcgaattc ggtaccaata aaagagcttt attttcatga tctgtgtgtt ggtttttgtg
     4741 tgcggcgcga taacttcgta tagctgaagt tcctatactt tctagagaat aggaacttcg
     4801 ctagcgcctt ggggggtggt gggggcgggg tctaggggtg gggtagagac tccagcccga
     4861 gggcagcaga gggacccggg cgtccgggcg agcaggtgtt ggggagggca gtggggcgcg
     4921 cgggctcagc gcgcgggtga gatgtggtct atattagagt atctatataa atatatattt
     4981 ccctggttcc tgtccctttt ccctgcccca acttctccct tgcgtctagg attgtactct
     5041 ctctgcccct cagcccagtc ccagtccctt cccgagtccc tagtgcatgg aataaagtgg
     5101 ttattaaatc cccgtgtgtc cccgagccag gggcctgcct ttatctcgac gtccacgccc
     5161 actttccctt cccttctgtc tcccaccctc agtcctgctc tccatggccc aagccccggg
     5221 gcagacaggt aagtaaagaa gagagcagag cgggaactga gatcgaaatt gaaaccaggt
     5281 ggaaagagag agatagggta gggggagaag ggatgggggc ctttaagaaa aaaacggata
     5341 aaaaggaaaa attgaaataa aatcgactct ggtgggattc gaacccacaa cctttgaatt
     5401 gctctattcg tcactagaag tccaatgcgc tatccattgc gccacagagc cacccgacga
     5461 acggcggcgt cttgtagctt acgggtacta gagtgggaat ggggcagggt tggggagcgg
     5521 ggctaaggga cttgggcggg acatgccagg agggcgcggt ttggatctca gaggccaagc
     5581 caggtagagg tagcgggcgc aaagcatgtt agccaggtga gagagagggc gcacatgggt
     5641 cgaaaaaaca gggagggaga gcaaccgaaa atggctgagc gagcgacgcg taaggcggta
     5701 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5761 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5821 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5881 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5941 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6001 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6061 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6121 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6181 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6241 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6301 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6361 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6421 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6481 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6541 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6601 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6661 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6721 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6781 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6841 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6901 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6961 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7021 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7081 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7141 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7201 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7261 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7321 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7381 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7501 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcaat
     7561 gccaatagga tatcggcatt ttcttttgcg tttttatttg ttaactgtta attgtccttg
     7621 ttcaaggatg ctgtctttga caacagatgt tttcttgcct ttgatgttca gcaggaagct
     7681 aggcgcaaac gttgattgtt tgtctgcgta gaatcctctg tttgtcatat agcttgtaat
     7741 cacgacattg tttcctttcg cttgaggtac agcgaagtgt gagtaagtaa aggttacatc
     7801 gttaggatca agatccattt ttaacacaag gccagttttg ttcagcggct tgtatgggcc
     7861 agttaaagaa ttagaaacat aaccaagcat gtaaatatcg ttagacgtaa tgccgtcaat
     7921 cgtcattttt gatccgcggg agtcagtgaa caggtaccat ttgccgttca ttttaaagac
     7981 gttcgcgcgt tcaatttcat ctgttactgt gttagatgca atcagcggtt tcatcacttt
     8041 tttcagtgtg taatcatcgt ttagctcaat cataccgaga gcgccgtttg ctaactcagc
     8101 cgtgcgtttt ttatcgcttt gcagaagttt ttgactttct tgacggaaga atgatgtgct
     8161 tttgccatag tatgctttgt taaataaaga ttcttcgcct tggtagccat cttcagttcc
     8221 agtgtttgct tcaaatacta agtatttgtg gcctttatct tctacgtagt gaggatctct
     8281 cagcgtatgg ttgtcgcctg agctgtagtt gccttcatcg atgaactgct gtacattttg
     8341 atacgttttt ccgtcaccgt caaagattga tttataatcc tctacaccgt tgatgttcaa
     8401 agagctgtct gatgctgata cgttaacttg tgcagttgtc agtgtttgtt tgccgtaatg
     8461 tttaccggag aaatcagtgt agaataaacg gatttttccg tcagatgtaa atgtggctga
     8521 acctgaccat tcttgtgttt ggtcttttag gatagaatca tttgcatcga atttgtcgct
     8581 gtctttaaag acgcggccag cgtttttcca gctgtcaata gaagtttcgc cgactttttg
     8641 atagaacatg taaatcgatg tgtcatccgc atttttagga tctccggcta atgcaaagac
     8701 gatgtggtag ccgtgatagt ttgcgacagt gccgtcagtg ttttgtaatg gccagctgtc
     8761 ccaaacgtcc aggccttttg cagaagagat atttttaatt gtggacgaat cgaactcagg
     8821 aactttatat ttttcatttt tttgctgttc agggatttgc agcatatcat ggcgtgtaat
     8881 atgggaaatg ccgtatgttt ccttatatgg cttttggttc gtttctttcg caaacgcttg
     8941 agttgcgcct cctgccagca gtgcggtagt aaaggttaat actgttgctt gttttgcaaa
     9001 ctttttgatg ttcatcgttc atgtctcctt ttttatgtac tgtgttagcg gtctgcttct
     9061 tccagccctc ctgtttgaag atggcaagtt agttacgcac aataaaaaaa gacctaaaat
     9121 atgtaagggg tgacgccaaa gtatacactt tgccctttac acattttagg tcttgcctgc
     9181 tttatcagta acaaacccgc gcgatttact tttcgacctc attctattag actctcgttt
     9241 ggattgcaac tggtctattt tcctcttttg tttgatagaa aatcataaaa ggatttgcag
     9301 actacgggcc taaagaacta aaaaatctat ctgtttcttt tcattctctg tattttttat
     9361 agtttctgtt gcatgggcat aaagttgcct ttttaatcac aattcagaaa atatcataat
     9421 atctcatttc actaaataat agtgaacggc aggtatatgt ggacgtcgcg gccgcataac
     9481 ttcgtataat gtatgctata cgaagttatg gatctttcag gagctaagga agctaaaatg
     9541 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     9601 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     9661 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     9721 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
     9781 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     9841 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     9901 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     9961 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
    10021 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
    10081 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
    10141 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
    10201 gatatcgagc tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct
    10261 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcac
    10321 tagtcatgac caggatctcc taggctagtt gtgattatga ttatgattat gattatgatt
    10381 atgaccactt acctgggccc gcgcgccaaa cgaatggtct agaaagctt
//
