LOCUS       sequence-25883-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for miRNA expression under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pSM2(CMV) (w57-1)
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           40..133
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           147..259
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(291..390)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(502..521)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(580..599)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcagatccgc tagtcgacta gggataacag ggtaattgtt tgaatgaggc ttcagtactt
       61 tacagaatcg ttgcctgcac atcttggaaa cacttgctgg gattacttct tcaggttaac
      121 ccaacagaag gctcgagcaa ccagaattca aggggctact ttaggagcaa ttatcttgtt
      181 tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg
      241 gtataaatta aatcactttt ttcaattgga agactaatgc gtttatctag acccagcttt
      301 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
      361 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
      421 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
      481 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
      541 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      601 atgtcgggca atcaggtgcg acaa
//
