LOCUS       sequence-258832-        8946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lysosomal marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Lim CY, Davis OB, Shin HR, Zhang J, Berdan CA, Jiang X, Counihan JL,
            Ory DS, Nomura DK, Zoncu R
  TITLE     ER-lysosome contacts enable cholesterol sensing by mTORC1 and drive 
            aberrant growth signalling in Niemann-Pick type C.
  JOURNAL   Nat Cell Biol. 2019 Oct;21(10):1206-1218. doi: 
            10.1038/s41556-019-0391-5. Epub 2019 Sep 23.
  PUBMED    31548609
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-019-0391-5"; journalName: "Nat Cell Biol"; date: 
            "2019-10"; volume: "21"; issue: "10"; pages: "1206-1218"
COMMENT     Sequence Label: pLJM1-FLAG-GFP-Tmem192
FEATURES             Location/Qualifiers
     source          1..8946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             378..401
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(417..419,420..1130)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(417..419,420..1130)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(459..480)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(720..739)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1067..1088
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2029..2146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2195..2705
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2624..2642
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2727..3326
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2727..2746)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3223..3243
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3454..3687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3759..3880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3796..3815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3850..3869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3920..4055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4075..4094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4076..4094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4104..4121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4104..4120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4113..4135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4262..4717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4349..4368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4559..4580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4743..4847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4848..4916,4917..5708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5066..5085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5879..6467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6368..6387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6621..6638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6755..6776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6791..6796,6797..6814,6815..6821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6829..6845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6834..6856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6853..6869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6853..6869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6888..6908
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6890..6908
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6936..7162
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7163..7343
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7390..7515
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8008..8241
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8426..8470
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8768..8788
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tgccatggac tacaaggatg acgatgacaa gggcggaggt ggatctgtga
      421 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      481 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      541 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      601 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      661 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      721 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      781 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      841 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      901 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      961 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1021 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1081 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggaggcggtg
     1141 gatcgggcgg gggtggatca aagtcgacga tggcggctgg aggcaggatg gaggacggtt
     1201 ccttggatat cacccagagt attgaagacg acccacttct ggatgcccag cttctcccac
     1261 accactcatt acaagctcac tttagacccc gattccatcc tcttcctaca gtcatcatag
     1321 tgaatcttct gtggtttatt catctcgtgt ttgttgtttt agcattttta acaggtgtgc
     1381 tttgttctta tcctaatcca aatgaggaca agtgcccagg aaattacaca aacccattga
     1441 aagttcagac ggttataatc cttgggaaag ttattttgtg gattctccat ttactccttg
     1501 aatgctacat ccagtatcac cacagcaaaa tcagaaaccg aggctataac ttgatctacc
     1561 gatcaacaag gcatctcaag agacttgcgt tgatgataca gtcctctggc aacacagtgc
     1621 ttctcctcat actgtgcatg cagcactcct tcccagagcc tggcagattg tatcttgacc
     1681 tcattctggc catcttggca ctggaactca tctgttccct gatatgtctc ctcatttaca
     1741 cagtgaaaat ccggagattt aataaagcta aaccagagcc tgatatactt gaagaagaaa
     1801 aaatctatgc ttaccccagc aatattacct cggagactgg attcagaact atttcaagcc
     1861 tagaagaaat tgttgaaaag caaggagaca ccattgaata cctgaagcga cacaatgcgc
     1921 tgctgagtaa gcgattgttg gctctcactt cctcagacct gggctgtcag ccaagtagaa
     1981 cgtgagagaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2041 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2101 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2161 ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag
     2221 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     2281 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     2341 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     2401 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2461 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2521 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2581 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2641 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2701 cccaggggga tccaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     2761 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2821 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     2881 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     2941 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     3001 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     3061 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     3121 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     3181 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     3241 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     3301 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     3361 ggagcgcacg accccatgca tcggtacctt taagaccaat gacttacaag gcagctgtag
     3421 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3481 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3541 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3601 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3661 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     3721 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3781 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3841 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3901 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3961 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4021 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4081 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4141 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4201 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4261 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4321 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4381 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4441 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4501 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4561 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4621 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4681 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6661 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6721 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6781 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6841 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6901 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6961 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7021 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7081 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7141 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7201 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7261 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7321 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7381 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7441 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7501 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7561 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7621 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7681 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7741 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7801 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7861 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7921 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7981 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8041 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8101 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8161 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8221 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8281 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8341 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8401 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8461 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8521 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8581 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8641 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     8701 tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgag cggccgcccc
     8761 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     8821 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     8881 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     8941 ctatca
//
