LOCUS       sequence-258836-        9714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Promoter 1 with bsa1 cut sites for inserting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Qu J, Prasad NK, Yu MA, Chen S, Lyden A, Herrera N, Silvis MR, 
            Crawford E, Looney MR, Peters JM, Rosenberg OS
  TITLE     Modulating pathogenesis with Mobile-CRISPRi.
  JOURNAL   J Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19.
  PUBMED    31481541
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19."
COMMENT     Sequence Label: PJQ47
FEATURES             Location/Qualifiers
     source          1..9714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  152..317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(472..561)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(571..2154,2155..2157,2158..4644,4645..4647,
                     4648..4674))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(571..4674)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             4681..4692
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(4698..4732)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     promoter        join(5165..5170,5171..5187,5188..5193)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    complement(5609..5656)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(5773..5778,5779..5795,5796..5801)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5990..6523
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    complement(6571..6618)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(6669..6755)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(6858..6952)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6980..6996)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6981..6997)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7411..7430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7530..7552
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7590..7608)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7676..7780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7781..7849,7850..8641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7999..8018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8942..9330
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9599..9707
                     /note="incP origin of transfer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
      121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
      181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
      241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
      301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
      361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc
      421 tggaatttgg taccgaggct gcaagtccca ttaaggaggc gcgccttatt acagatcttc
      481 ctcagagatg agcttctgtt ccagatcttc ttctgaaatc aacttttgtt ccagatcttc
      541 ttcagagatg agtttctgct ccgcggccgc gtcgcctccc agctgagaca ggtcgatccg
      601 tgtctcgtac aggccggtga tgctctggtg gatcagggtg gcgtccagca cctctttggt
      661 gctggtgtac ctcttccggt cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc
      721 cagattggtc agggtaaaca ggtggatgat attctcggcc tgctctctga taggcttgtc
      781 tctgtgcttg ttgtaggcgc tcagcacctt gtccagatta gcgtcggcca ggatcactct
      841 cttggagaac tcgctgatct gctcgatgat ctcgtccagg tagtgtttgt gctgttccac
      901 aaacagctgt ttctgctcat tatcctcggg ggagcccttc agcttctcat agtggctggc
      961 caggtacagg aagttcacat atttggaggg cagggccagt tcgtttccct tctgcagttc
     1021 gccggcagag gccagcattc tcttccggcc gttttccagc tcgaacaggg agtacttagg
     1081 cagcttgatg atcaggtcct ttttcacttc tttgtagccc ttggcttcca gaaagtcgat
     1141 gggattcttc tcgaagctgc ttctttccat gatggtgatc cccagcagct ctttcacact
     1201 cttcagtttc ttggacttgc ccttttccac tttggccacc accagcacag aataggccac
     1261 ggtggggctg tcgaagccgc cgtacttctt agggtcccag tccttctttc tggcgatcag
     1321 cttgtcgctg ttcctcttgg gcaggataga ctctttgctg aagccgcctg tctgcacctc
     1381 ggtctttttc acgatattca cttggggcat agacagcact ttccgcacgg tggcaaagtc
     1441 ccggccctta tcccacacga tctcgcctgt ttcgccgttt gtctcgatca gaggccgctt
     1501 ccggatctcg ccgttggcca gggtaatctc ggtcttgaaa aagttcatga tgttgctgta
     1561 gaagaagtac ttggcggtag ccttgccgat ttcctgctcg ctcttggcga tcatcttccg
     1621 cacgtcgtac accttgtagt cgccgtacac gaactcgctt tccagcttag ggtacttttt
     1681 gatcagggcg gttcccacga cggcgttcag gtaggcgtcg tgggcgtggt ggtagttgtt
     1741 gatctcgcgc actttgtaaa actggaaatc cttccggaaa tcggacacca gcttggactt
     1801 cagggtgatc actttcactt cccggatcag tttgtcgttc tcgtcgtact tagtgttcat
     1861 ccgggagtcc aggatctgtg ccacgtgctt tgtgatctgc cgggtttcca ccagctgtct
     1921 cttgatgaag ccggccttat ccagttcgct caggccgcct ctctcggcct tggtcagatt
     1981 gtcgaacttc ctctgggtaa tcagcttggc attcagcagc tggcgccagt agttcttcat
     2041 cttcttcacg acctcttcgg agggcacgtt gtcgctcttg ccccggttct tgtcgctccg
     2101 agtcagcact ttgttatcga tggagtcgtc cttcagaaag ctctgaggca cgatagcgtc
     2161 cacatcgtag tcggacagcc ggttgatgtc cagttcctgg tccacgtaca tatcccgccc
     2221 attctgcagg tagtacaggt acagcttctc gttctgcagc tgggtgtttt ccacggggtg
     2281 ttctttcagg atctggctgc ccagctcttt gatgccctct tcgatccgct tcattctctc
     2341 gcggctgttc ttctgtccct tctgggtggt ctggttctct ctggccattt cgatcacgat
     2401 gttctcgggc ttgtgccggc ccatcacttt cacgagctcg tccaccacct tcactgtctg
     2461 caggatgccc ttcttaatgg cggggctgcc ggccagattg gcaatgtgct cgtgcaggct
     2521 atcgccctgg ccggacacct gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc
     2581 gtggatcagc tgcatgaagt ttctgttggc gaagccgtcg gacttcagga aatccaggat
     2641 tgtcttgccg gactgcttgt cccggatgcc gttgatcagc ttccggctca gcctgcccca
     2701 gccggtgtat ctccgccgct tcagctgctt catcactttg tcgtcgaaca ggtgggcata
     2761 ggttttcagc cgttcctcga tcatctctct gtcctcaaac agtgtcaggg tcagcacgat
     2821 atcttccaga atgtcctcgt tttcctcatt gtccaggaag tccttgtcct tgataatttt
     2881 cagcagatcg tggtatgtgc ccagggaggc gttgaaccga tcttccacgc cggagatttc
     2941 cacggagtcg aagcactcga ttttcttgaa gtagtcctct ttcagctgct tcacggtcac
     3001 tttccggttg gtcttgaaca gcaggtccac gatggctttt ttctgctcgc cgctcaggaa
     3061 ggcgggcttt ctcattccct cggtcacgta tttcactttg gtcagctcgt tgtacacggt
     3121 gaagtactcg tacagcaggc tgtgcttggg cagcaccttc tcgttgggca ggttcttatc
     3181 gaagttggtc atccgctcga tgaagctctg ggcgctggcg cccttgtcca ccacttcctc
     3241 gaagttccag ggggtgatgg tttcctcgct ctttctggtc atccaggcga atctgctgtt
     3301 tcccctggcc agagggccca cgtagtaggg gatgcggaag gtcaggatct tctcgatctt
     3361 ttcccggttg tccttcagga atgggtaaaa atcttcctgc cgccgcagaa tggcgtgcag
     3421 ctctcccagg tggatctggt gggggatgct gccgttgtcg aaggtccgct gcttccgcag
     3481 caggtcctct ctgttcagct tcacgagcag ttcctcggtg ccgtccatct tttccaggat
     3541 gggcttgatg aacttgtaga actcttcctg gctggctccg ccatcgatgt agccggcgta
     3601 gccgttcttg ctctggtcga agaaaatctc tttgtacttc tcaggcagct gctgccgcac
     3661 gagagctttc agcagggtca ggtcctggtg gtgctcgtcg tatctcttga tcatagaggc
     3721 gctcaggggg gccttggtga tctcggtgtt cactctcagg atgtcgctca gcaggatggc
     3781 gtcggacagg ttcttggcgg ccagaaacag gtcggcgtac tggtcgccga tctgggccag
     3841 caggttgtcc aggtcgtcgt cgtaggtgtc cttgctcagc tgcagtttgg catcctcggc
     3901 caggtcgaag ttgctcttga agttgggggt caggcccagg ctcagggcaa tcaggttgcc
     3961 gaacaggcca ttcttcttct cgccgggcag ctgggcgatc agattttcca gccgtctgct
     4021 cttgctcagt ctggcagaca ggatggcctt ggcgtccacg ccgctggcgt tgatggggtt
     4081 ttcctcgaac agctggttgt aggtctgcac cagctggatg aacagcttgt ccacgtcgct
     4141 gttgtcgggg ttcaggtcgc cctcgatcag gaagtggccc cggaacttga tcatgtgggc
     4201 cagggccaga tagatcagcc gcaggtcggc cttgtcggtg ctgtccacca gtttctttct
     4261 caggtggtag atggtggggt acttctcgtg gtaggccacc tcgtccacga tgttgccgaa
     4321 gatggggtgc cgctcgtgct tcttatcctc ttccaccagg aaggactctt ccagtctgtg
     4381 gaagaagctg tcgtccacct tggccatctc gttgctgaag atctcttgca gatagcagat
     4441 ccggttcttc cgtctggtgt atcttcttct ggcggttctc ttcagccggg tggcctcggc
     4501 tgtttctccg ctgtcgaaca gcagggcgcc gatcaggttc ttcttgatgc tgtgccggtc
     4561 ggtgttgccc agcaccttga atttcttgct gggcaccttg tactcgtcgg tgatcacggc
     4621 ccagcccaca gagttggtgc cgatggccag gccgatgctg tacttcttgt ccatactagt
     4681 tttctcctct ttagatcgct agcacaatcc ctaggactga gctagctgtc aagaaccgag
     4741 gtaactggct tggtgataag ctgtcaaacc agatcaattc gcactagtct gcagacgtaa
     4801 aaaaagcggc gtggttagcc gcttttttaa ttgccggagc gcaataaaaa agcccccgga
     4861 aggtgatctt ccgggggctt tctcatgcgt tgcttgtatt gaaaatgggg gccatgccta
     4921 cctatctgcc tcccgggtcc ttgagcccca ttatcacctc caaagccttc tcgtctggtc
     4981 agtttcacct gttttacgta aaaacccgct tcggcgggtt tttacttttg gggaaacaca
     5041 gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg accaaaggtg cgactactct
     5101 tgcctactac ctatcgactg agctgaaaga attccggttc tggcaaatat tctgaaatga
     5161 gctgttgaca attaatcatc cggctcgtat aattctagtt gagaccaact ttggtctcca
     5221 ccatagcggt cggtctctgt ttaagagcta tgctggaaac agcatagcaa gtttaaataa
     5281 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tttgaattca
     5341 tgtggctgac cgttctgttg tctctcgctc ttccgagtag acgaacaata aggcctccct
     5401 aacggggggc cttttttatt gataacaaaa gtcagtgctt ccgctatttc caaaataccg
     5461 ggctaatacg gtttaaacga cctcctggat ttgctcagac agccttttcg tcattcgttt
     5521 cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg cggtggacag
     5581 gatcggcggt tttcttttct cttctcaaga agttcctata ctttctagag aataggaact
     5641 tcggaatagg aacttcctcc tgaacggcca taagaacgaa ggctgtctgt tgaactctcg
     5701 agccttgtcg ccttgcgtat aatatttgcc catggacgca caccgtggaa acggatgaag
     5761 gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
     5821 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     5881 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
     5941 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     6001 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
     6061 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
     6121 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
     6181 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
     6241 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag
     6301 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac
     6361 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
     6421 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
     6481 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
     6541 gatctcgagc atcagcttca aaagcgctct gaagttccta tactttctag agaataggaa
     6601 cttcggaata ggtacttcaa gatccccaat tcgagctcat cgtccgggcc gcaagctcct
     6661 agcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     6721 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tcaagctaga gagtcattac
     6781 cccaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     6841 atcgcagtct agcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     6901 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagctca
     6961 gctaattaag ctagcttatc gataccgtcg acctcgaacc ccacgcccct ctttaatacg
     7021 acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta
     7081 tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga
     7141 ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac
     7201 ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg
     7261 ctggtcctct agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7321 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7381 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7441 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7501 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7561 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7621 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7801 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7861 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7921 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7981 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8041 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8101 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8161 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8221 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8281 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8341 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8401 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8461 gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca ttgcagcact
     8521 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8581 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8641 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8701 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggcagtt attggtgcct
     8821 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8881 taaaacttca tttttaattt ttgcggccgc aagatccggc cacgatgcgt ccggcgtaga
     8941 ggatctgaag atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg
     9001 tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg
     9061 ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc
     9121 tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa
     9181 gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt
     9241 ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt
     9301 gagtgacaca ggaacactta acggctgaca tgggaattag ccatggcatc acagtatcgt
     9361 gatgacagag gcagggagtg cggccttttt acggttcctg gccttttgct ggccttttgc
     9421 tcacatgttc tttcctgcgt tatcagggga ttccttaagg tatactttcc gctgcataac
     9481 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     9541 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     9601 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     9661 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgata agct
//
