LOCUS       sequence-258840-        9714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Promoter 2 with bsa1 cut sites for inserting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Qu J, Prasad NK, Yu MA, Chen S, Lyden A, Herrera N, Silvis MR, 
            Crawford E, Looney MR, Peters JM, Rosenberg OS
  TITLE     Modulating pathogenesis with Mobile-CRISPRi.
  JOURNAL   J Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19.
  PUBMED    31481541
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19."
COMMENT     Sequence Label: PJQ48
FEATURES             Location/Qualifiers
     source          1..9714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            8..116
                     /note="incP origin of transfer"
     rep_origin      385..773
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(1074..1865,1866..1934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1697..1716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2107..2125
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2163..2185)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2285..2304
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2718..2734
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2719..2735
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2763..2857
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      2960..3046
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3097..3144
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3192..3725)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3914..3919,3920..3936,3937..3942))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    4059..4106
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(join(4522..4527,4528..4544,4545..4550))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        4983..5017
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     RBS             5023..5034
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5041..5067,5068..5070,5071..7557,7558..7560,
                     7561..9144)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5041..9144
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9154..9243
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     mobile_element  9398..9563
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
       61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
      121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
      181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
      241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa
      301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata
      361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
      661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
      721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
      781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa
      841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt
     2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct
     2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg
     2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc
     3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta
     3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct
     3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     3361 atgccctgcc tccggtgctc tccggagact gcgagatcat agatatagat ctcactacgc
     3421 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat
     3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag
     4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc
     4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc
     4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga
     4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt aaaccgtatt agcccggtat
     4261 tttggaaata gcggaagcac tgacttttgt tatcaataaa aaaggccccc cgttagggag
     4321 gccttattgt tcgtctactc ggaagagcga gagacaacag aacggtcagc cacatgaatt
     4381 caaaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt
     4441 aaacttgcta tgctgtttcc agcatagctc ttaaacagag accgaccgct atggtggaga
     4501 ccaaagttgg tctcaactag aattatacga gccggatgat taattgtcaa cagctcattt
     4561 cagaatattt gccagaaccg gaattctttc agctcagtcg ataggtagta ggcaagagta
     4621 gtcgcacctt tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt
     4681 tccccaaaag taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccag
     4741 acgagaaggc tttggaggtg ataatggggc tcaaggaccc gggaggcaga taggtaggca
     4801 tggcccccat tttcaataca agcaacgcat gagaaagccc ccggaagatc accttccggg
     4861 ggctttttta ttgcgctccg gcaattaaaa aagcggctaa ccacgccgct ttttttacgt
     4921 ctgcagacta gtgcgaattg atctggtttg acagcttatc accaagccag ttacctcggt
     4981 tctttatggc tagctcagtc ctaggtacaa tgctagcgat ctaaagagga gaaaactagt
     5041 atggacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
     5101 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     5161 cacagcatca agaagaacct gatcggcgcc ctgctgttcg acagcggaga aacagccgag
     5221 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     5281 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     5341 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     5401 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     5461 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     5521 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     5581 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     5641 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     5701 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
     5761 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     5821 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     5881 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     5941 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     6001 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     6061 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     6121 ggctacatcg atggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     6181 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     6241 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     6301 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     6361 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     6421 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     6481 gtggtggaca agggcgccag cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     6541 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     6601 tacaacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     6661 agcggcgagc agaaaaaagc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     6721 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     6781 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     6841 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     6901 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     6961 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     7021 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     7081 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     7141 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     7201 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     7261 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     7321 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     7381 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     7441 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     7501 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggacgct
     7561 atcgtgcctc agagctttct gaaggacgac tccatcgata acaaagtgct gactcggagc
     7621 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     7681 aactactggc gccagctgct gaatgccaag ctgattaccc agaggaagtt cgacaatctg
     7741 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     7801 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     7861 actaagtacg acgagaacga caaactgatc cgggaagtga aagtgatcac cctgaagtcc
     7921 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     7981 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     8041 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     8101 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     8161 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     8221 cctctgatcg agacaaacgg cgaaacaggc gagatcgtgt gggataaggg ccgggacttt
     8281 gccaccgtgc ggaaagtgct gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     8341 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga caagctgatc
     8401 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     8461 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     8521 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     8581 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     8641 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     8701 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     8761 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     8821 cagcacaaac actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     8881 atcctggccg acgctaatct ggacaaggtg ctgagcgcct acaacaagca cagagacaag
     8941 cctatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     9001 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     9061 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     9121 gacctgtctc agctgggagg cgacgcggcc gcggagcaga aactcatctc tgaagaagat
     9181 ctggaacaaa agttgatttc agaagaagat ctggaacaga agctcatctc tgaggaagat
     9241 ctgtaataag gcgcgcctcc ttaatgggac ttgcagcctc ggtaccaaat tccagaaaag
     9301 acacccgaaa gggtgttttt tcgttttggt cccacagaat gagcatcatg gcctagtcga
     9361 catcggagaa gagtacggct cttttaaccg cctcaagaac cagataagtg aaatctagtt
     9421 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     9481 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     9541 cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt tgggcgctgc
     9601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9661 cctcgctcac tgacccgctg cgctcggtcg ttcggctgcg gcgagcggta tcag
//
