LOCUS       sequence-258850-        1242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged plasma membrane marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..1242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    418..991
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(585..602)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     812..831
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ccgtatggcg ccgggacctc tcggcatgga cgagctgtac aagtactcag atctcgacat
       61 ggcaggaata gagttggagc ggtgccagca gcaggcgaac gaggtgacgg aaattatgcg
      121 taacaacttc ggcaaggtcc tggagcgtgg tgtgaagctg gccgaactgc agcagcgttc
      181 agaccaactc ctggatatga gctcaacctt caacaagact acacagaacc tggcccagaa
      241 gaagtgctgg gagaacatcc gttaccggat ctgcgtgggg ctggtggtgg ttggtgtcct
      301 gctcatcatc ctgattgtgc tgctggtcgt ctttctccct cagagcagtg acagcagtag
      361 tgccccacgg acccaggatg caggcattgc ctcagggcct gggaactgag gatcccgccc
      421 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
      481 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
      541 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
      601 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
      661 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
      721 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
      781 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
      841 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
      901 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
      961 cgtggttttc ctttgaaaaa cacgatgata agcttgccac acccacaagg agacgacctt
     1021 ccatgaccga gtacagccca cgtgcgctcg ccacccgcga cgacgtcccc gggcgtacgc
     1081 ccctcgccgc cgcgttcgcg actacccgca cgcgcacacg tcgacccgac gcacatcgag
     1141 ggtcacgagc tgcagactct cctcacgcgc gtcgactcga atcggcaagg ttagtccgga
     1201 cgacggcccc ggtgacgttc tggaaccacc ccgggaaaag cg
//
