LOCUS       sequence-258855-       12461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UASp construct for over-expression of Drosophila Crk with N-terminal
            tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated 
            site-specific integration or classical P-element insertion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Spracklen AJ, Thornton-Kolbe EM, Bonner AN, Florea A, Compton PJ, 
            Fernandez-Gonzalez R, Peifer M
  TITLE     The Crk adapter protein is essential for Drosophila embryogenesis, 
            where it regulates multiple actin-dependent morphogenic events.
  JOURNAL   Mol Biol Cell. 2019 Jul 18:mbcE19050302. doi: 
            10.1091/mbc.E19-05-0302.
  PUBMED    31318326
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2019 Jul 18:mbcE19050302. doi: 10.1091/mbc.E19-05-0302."
COMMENT     Sequence Label: pTIGER_tdTomato::3xFLAG::dCrk
FEATURES             Location/Qualifiers
     source          1..12461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     CDS             complement(join(1365..1979,2050..2181,2385..2700,2762..3416,
                     3491..3764,4166..4237))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4689..4707
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             5409..6836
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             6849..6914
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    9235..9304
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(9468..9669,9670..9700)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10238..10257)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10357..10379
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10417..10435)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10503..10607
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10608..10676,10677..11468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10826..10845)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11639..12227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12128..12147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     1381 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     1441 cccacgtagt ccagcggcag atcggcggcg gagaagttaa gcgtctccag gatgaccttg
     1501 cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa tttcgcccgg ctccacgtcc
     1561 gcccattggt taatcagcag accctcgttg gcgtaacgga accatgagag gtacgacaac
     1621 catttgaggt atactggcac cgagcccgag ttcaagaaga agccgccaaa gagcaggaat
     1681 ggtatgataa ccggcggacc cacagacagc gccatcgagg tcgaggagct ggcgcaggat
     1741 attagatatc cgaaggacgt tgacacattg gccaccagag tgaccagcgc caggcagttg
     1801 aagaagtgca gcactccggc ccgcagtccg atcatcggat aggcaatcgc cgtgaagacc
     1861 agtggcactg tgagaaaaag cggcaattcg gcaatcgttt tgcccagaaa gtatgtgtca
     1921 cagcgataaa gtcgacttcg ggcctccctc ataaaaactg gcagctctga ggtgaacacc
     1981 taaatcgaat cgattcatta gaaagttagt aaattattga aatgcaaatg tattctaaac
     2041 atgacttaca tttatcgtgg caaagacgtt ttgaaaggtc atgttggtca ggaagaggaa
     2101 gatggctccg ttgatattca tcacacccac ttgcgtgagt tgttggccca aaaagatgag
     2161 gccaatcaag atggcaacca tctgcaaatt aaaatgttac tcgcatctca ttaatattcg
     2221 cgagttaaat gaaatttatt tatcttctgc aaaactataa actatacatc tcattgaaaa
     2281 aaactaagaa gggtgtggaa tcaggcaatt ctatctaaaa tctagcgaat ttgtttccaa
     2341 gaattgtaag cgttatatca tttgtttcca ctggaaccac tcaccgttgt ctgaataagt
     2401 cgcactttta cgaggagtgg ttccttgagc accgacagcc aggatcgcca caggaccgcc
     2461 cggaactgca tgaaccaggt ggccttgtag gtgtacccat tctccggctg ctccagtggc
     2521 ttctccagat ttttggtggc caacaactgc tccatatccc gggctacttt gctaatggca
     2581 aaattgtcgc atatcttggc gatccgatca cgggactcga tctcccgtcc gggcacaacg
     2641 gccaacacct gtacgtaaaa gtccgccgga ttgtagttgg taggacactg ggcacccacg
     2701 ctggatagga gttgagatgt tatgtaatac tagataccct taataaacac atcgaactca
     2761 ctaggaaaag aagtcgacgg cttcgctggg agtgcccaag aaagctaccc tgccctcggc
     2821 catcagaagg atcttgtcaa agagctcaaa cagctcggaa gacggctgat gaatggtcag
     2881 gatgacggtc ttgcccttct gcgacagctt cttcagcacc tggacgacgc tgtgggcggt
     2941 aaaggagtcc agtccggagg tgggctcatc gcagatcaga agcggcggat cggttagagc
     3001 ctcggaggcg aatgccagac gcttcctttc tccgccggac agacctttca ccctgccggg
     3061 cacaccgatg atcgtgtgct gacatttgct gagcgaaagc tcctggatca cctgatccac
     3121 gcgggccact cgctgccgat aggtcagatg tcgtggcatc cgcaccatgg cttggaaaat
     3181 caggtgttcc ctggccgtta gggagccgat aaagaggtca tcctgctgga cataggcgca
     3241 cctggcctgc atctccttgg cgtccacagg ttggccattg agcagtcgca tcccggatgg
     3301 cgatacttgg atgccctgcg gcgatcgaaa ggcaagggca ttcagcaggg tcgtctttcc
     3361 ggcaccggaa ctgcccatca cggccaaaag ttcgcccgga taggccacgc cgcaaactga
     3421 gtttcaaatt ggtaattgga ccctttatta agatttcaca cagatcagcc gactgcgaat
     3481 agaaactcac cgttcttgag caaatgtttc ctgggcgccg gtatgtgtcg ctcgttgcag
     3541 aatagtccgc gtgtccggtt gaccagctgc cgccatccgg agcccggctg attgaccgcc
     3601 ccaaagatgt ccatattgtg ccaggcatag gtgaggttct cggctagttg gccgctccct
     3661 gaaccggagt cctccggcgg actgggtggc cggagcgtgc cgtagttttt ggcctgcccg
     3721 aagccctggt taatgcagct ctgcgaagcc gctccgctgt caccctgcaa tgatagggga
     3781 tctcaaatat caactacaag cgttatgctc atctaacccc gaacaaaaag taccccgaag
     3841 tatcctacga agtaggttta tacttttatt tattttttgt gcatctagga tcagcttaaa
     3901 atatctggtt gttatatttt ttgtaaaaaa gaatatagtc gaaaatgaat gcctttagat
     3961 gtcttgatca tgatatgatc tcaaaaattg tcttatatag cgagaacagc taccagaata
     4021 atctgtttcg tgtcactatt tgtttgtgca attgcggttt gggatttttg tgggtcgcag
     4081 ttctcacgcc gcagacaatt tgatgttgca atcgcagttc ctatagatca agtgaactta
     4141 agatgtatgc acatgtacta ctcacattgt tcagatgctc ggcagatggg tgtttgctgc
     4201 ctccgcgaat taatagctcc tgatcctctt ggcccattgc cgggattttt cacactttcc
     4261 cctgcttacc cacccaaaac caatcaccac cccaatcact caaaaaacaa acaaaaataa
     4321 gaagcgagag gagttttggc acagcacttt gtgtttaatt gatggcgtaa accgcttgga
     4381 gcttcgtcac gaaaccgctg acaaaatgca actgaaggcg gacattgacg ctacgtaacg
     4441 ctacaaacgg tggcgaaaga gatagcggac gcagcggcga aagagacggc gatatttctg
     4501 tggacagaga aggaggcaaa cagcgctgac tttgagtgga atgtcatttt gagtgagagg
     4561 taatcgaaag aacctggtac atcaaatacc cttggatcga agtaaattta aaactgatca
     4621 gataagttca atgatatcca gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc
     4681 gcaaaaatgg gttttattaa cttacataca tactagaatt ggccgctcta gccccccctc
     4741 gaatgttctc tctcttctct tctctctctc tttctcgaat gttctctctc ttctcttctc
     4801 tctctctttc tcgaggtcat caagcttagg cctccaaggc ggagtactgt cctccgggct
     4861 ggcggagtac tgtcctccgg caaggcggag tactgtcctc cgggctggcg gagtactgtc
     4921 ctccggcaag gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaaggcgg
     4981 agtactgtcc tccgggctgg cggagtactg tcctccggca aggcggagta ctgtcctccg
     5041 ggctggcgga gtactgtcct ccggcaaggc ggagtactgt cctccgggct ggcggagtac
     5101 tgtcctccgg caaggcggag tactgtcctc cgggctggcg gagtactgtc ctccggcaag
     5161 ggtcgagtcg atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt
     5221 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaaccggtga tagagcctga
     5281 accagaaaag ataaaagaag gctataccag tgggagtaca caaacagagt aagtttgaat
     5341 agtaaaaaaa atcatttatg taaacaataa cgtgactgtg cgttaggtcc tgttcattgg
     5401 tacccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     5461 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     5521 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     5581 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     5641 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     5701 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     5761 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     5821 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     5881 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     5941 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6001 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6061 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     6121 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     6181 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     6241 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     6301 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     6361 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     6421 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     6481 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     6541 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     6601 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     6661 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     6721 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6781 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaaggcgg
     6841 ccgcaatgga ctacaaggat catgacggtg actacaaaga ccatgatata gattacaagg
     6901 atgacgacga caagatggat acatttgacg tttctgatag gaacagctgg tactttggtc
     6961 ccatgtctag acaggatgct actgaagttt tgatgaacga acgcgagcgg ggagtgtttt
     7021 tagtccgtga tagtaactcg atagcagggg attatgtact ttgtgtaaga gaagatacaa
     7081 aagttagcaa ctacatcatt aacaaagttc aacaacagga tcaaatcgtt taccgcattg
     7141 gggatcagtc ttttgacaat ctaccgaaac tcttaacttt ttacactctt cattatttgg
     7201 atacaacccc tttaaaacgg cctgcgtgta gaagggtgga aaaagtaata ggaaagttcg
     7261 atttcgttgg cagcgatcaa gatgatttac cttttcaaag aggtgaagtt ttaacaatag
     7321 ttcgaaaaga cgaggatcaa tggtggactg cgcgtaactc ctcggggaaa attggtcaaa
     7381 taccggttcc ctatatacaa cagtatgacg attatatgga tgaagatgct attgataaaa
     7441 acgaaccttc catttcggga tctagcaatg tatttgaaag tactcttaaa aggacagatt
     7501 taaatcgaaa actacctgca tacgcccgcg taaaacagtc aagggtccct aacgcatacg
     7561 ataagactgc attaaaattg gaaataggtg acattattaa agtcactaaa acaaacatta
     7621 atgggcaatg ggagggagaa ttaaatggaa aaaatggtca ttttcccttc acgcacgttg
     7681 aatttgtcga tgattgtgat ttaagcaaaa actccacaga aatatgctaa gcatgcatct
     7741 agagtcgacc tcgaacgtta acgttaacgt aacgttaact cgaggagctt gataacatta
     7801 tacctaaacc catggtcaag agtaaacatt tctgcctttg aagttgagaa cacaattaag
     7861 catcccctgg ttaaacctga cattcatact tgttaatagc gccataaaca tagcaccaat
     7921 ttcgaagcat acttgttaat agcgccataa acatagcacc aatttcgaag aaatcagtta
     7981 aaagcaatta gcaattagca attagcaata actctgctga cttcaaaacg agaagagttg
     8041 caagtatttg taaggcacag tttatagacc accgacggct cattagggct cgtcatgtaa
     8101 ctaagcgcgg tgaaacccaa ttgaacatat agtggaatta ttattatcaa tggggaagat
     8161 ttaaccctca ggtagcaaag taatttaatt gcaaatagag agtcctaaga ctaaataata
     8221 tatttaaaaa tctggccctt tgaccttgct tgtcaggtgc atttgggttc aatcgtaagt
     8281 tgcttctata taaacacttt ccccatcccc gcaataatga agaataccgc agaataaaga
     8341 gagatttgca acaaaaaata aaggcattgc gaaaactttt tatgggggat cattacactc
     8401 gggcctacgg ttacaattcc cagccactta agcgacaagt ttggccaaca atccatctaa
     8461 tagctaatag cgcaatcact ggtaatcgca agagtatata ggcaatagaa cccatggatt
     8521 tgaccaaagg taaccgagac aatggagaag caagaggatt tcaaactgaa cacccacagt
     8581 gctgtgtact accactggcg cgtttgggag ctcactggcc tgatgcgtcc tccgggcgtt
     8641 tcaagcctgc tttacgtggt atactccatt acggtcaact tggtggtcac cgtgctgttt
     8701 cccttgagct tgctggccag gctgctgttc accaccaaca tggccggatt gtgcgagaac
     8761 ctgaccataa ctattaccga tattgtggcc aatttgaagt ttgcgaatgt gtacatggtg
     8821 aggaagcagc tccatgagat tcgctctctc ctaaggctca tggacgctag agcccggctg
     8881 gtgggcgatc ccgaggagat ttctgccttg aggaaggaag tgaatatcgc acagggcact
     8941 ttccgcacct ttgccagtat tttcgtattt ggcactactt tgagttgcgt ccgcgtggtc
     9001 gttcgcccgg atcgagagct cctgtatccg gcctggttcg gcgttgactg gatgcactcc
     9061 accagaaact atgtgctcat caatatctac cagctcttcg gcttgatagt gcaggctata
     9121 cagaactgcg ctagtgactc ctatccgcct gcgtttctct gcctgctcac gggtcatatg
     9181 cgtgctttgg agctgagggt gcggcggatt ggcgtcgacg atgtaggtca cggtctcgaa
     9241 gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc
     9301 catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg
     9361 gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg
     9421 acgtgcgacg gcgtcgacga tgtaggtcac ggcgggcagc caagctttgc gtactcgcaa
     9481 attattaaaa ataaaacttt aaaaataatt tcgtctaatt aatattatga gttaattcaa
     9541 accccacgga catgctaagg gttaatcaac aatcatatcg ctgtctcact cagactcaat
     9601 acgacactca gaatactatt cctttcactc gcacttattg caagcatacg ttaagtggat
     9661 gtctcttgcc gacgggacca ccttatgtta tttcatcatg gtctggccat tctcatcgtg
     9721 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     9781 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     9841 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     9901 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     9961 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
    10021 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
    10081 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
    10141 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
    10201 ggtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
    10261 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    10321 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    10381 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
    10441 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    10501 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10561 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10621 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10681 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10741 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10801 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10861 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10921 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10981 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11041 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11101 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11161 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11221 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11281 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11341 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11401 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11461 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccttcttct
    12241 tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt
    12301 acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct
    12361 gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact
    12421 tctccgccgc cgatctgccg ctggactacg tgggtctggc c
//
