LOCUS       sequence-258861-       11732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UASp construct for over-expression of Drosophila Crk with N-terminal
            monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated
            site-specific integration or classical P-element insertion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Spracklen AJ, Thornton-Kolbe EM, Bonner AN, Florea A, Compton PJ, 
            Fernandez-Gonzalez R, Peifer M
  TITLE     The Crk adapter protein is essential for Drosophila embryogenesis, 
            where it regulates multiple actin-dependent morphogenic events.
  JOURNAL   Mol Biol Cell. 2019 Jul 18:mbcE19050302. doi: 
            10.1091/mbc.E19-05-0302.
  PUBMED    31318326
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2019 Jul 18:mbcE19050302. doi: 10.1091/mbc.E19-05-0302."
COMMENT     Sequence Label: pTIGER_mNeonGreen::3xFLAG::dCrk
FEATURES             Location/Qualifiers
     source          1..11732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(769..1383,1454..1585,1789..2104,2166..2820,
                     2895..3168,3570..3641))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4093..4111
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     regulatory      5381..5390
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5524..5589
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    7910..7979
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(8143..8344,8345..8375)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(8913..8932)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9032..9054
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9092..9110)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9178..9282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9283..9351,9352..10143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9501..9520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10314..10902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10803..10822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(11137..11167,11168..11722)
                     /label=P element 5' end
ORIGIN
        1 ctagttccag tgaaatccaa gcattttcta aattaaatgt attcttatta ttatagttgt
       61 tatttttgat atatataaac aacactatta tgcccaccat ttttttgaga tgcatctaca
      121 caaggaacaa acactggatg tcactttcag ttcaaattgt aacgctaatc actccgaaca
      181 ggtcacaaaa aattacctta aaaagtcata atattaaatt agaataaata tagctgtgag
      241 ggaaatatat acaaatatat tggagcaaat aaattgtaca tacaaatatt tattactaat
      301 ttctattgag acgaaatgaa ccactcggaa ccatttgagc gaaccgaatc gcgcggaact
      361 aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg
      421 agacagcgaa agagcaacta cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc
      481 gcgatttgaa aagtatgtat ataaaaaata tatcccggtg ttttatgtag cgataaacga
      541 gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc
      601 tacttgtggg gatgttcgaa ggggaaatac ttgtattcta taggtcatat cttgttttta
      661 ttggcacaaa tataattaca ttagcttttt gagggggcaa taaacagtaa acacgatggt
      721 aataatggta aaaaaaaaaa acaagcagtt atttcggata tatgtcggct actccttgcg
      781 tcgggcccga agtcttagag ccagatatgc gagcacccgg aagctcacga tgagaatggc
      841 cagacccacg tagtccagcg gcagatcggc ggcggagaag ttaagcgtct ccaggatgac
      901 cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc ccggctccac
      961 gtccgcccat tggttaatca gcagaccctc gttggcgtaa cggaaccatg agaggtacga
     1021 caaccatttg aggtatactg gcaccgagcc cgagttcaag aagaagccgc caaagagcag
     1081 gaatggtatg ataaccggcg gacccacaga cagcgccatc gaggtcgagg agctggcgca
     1141 ggatattaga tatccgaagg acgttgacac attggccacc agagtgacca gcgccaggca
     1201 gttgaagaag tgcagcactc cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa
     1261 gaccagtggc actgtgagaa aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt
     1321 gtcacagcga taaagtcgac ttcgggcctc cctcataaaa actggcagct ctgaggtgaa
     1381 cacctaaatc gaatcgattc attagaaagt tagtaaatta ttgaaatgca aatgtattct
     1441 aaacatgact tacatttatc gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga
     1501 ggaagatggc tccgttgata ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga
     1561 tgaggccaat caagatggca accatctgca aattaaaatg ttactcgcat ctcattaata
     1621 ttcgcgagtt aaatgaaatt tatttatctt ctgcaaaact ataaactata catctcattg
     1681 aaaaaaacta agaagggtgt ggaatcaggc aattctatct aaaatctagc gaatttgttt
     1741 ccaagaattg taagcgttat atcatttgtt tccactggaa ccactcaccg ttgtctgaat
     1801 aagtcgcact tttacgagga gtggttcctt gagcaccgac agccaggatc gccacaggac
     1861 cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac ccattctccg gctgctccag
     1921 tggcttctcc agatttttgg tggccaacaa ctgctccata tcccgggcta ctttgctaat
     1981 ggcaaaattg tcgcatatct tggcgatccg atcacgggac tcgatctccc gtccgggcac
     2041 aacggccaac acctgtacgt aaaagtccgc cggattgtag ttggtaggac actgggcacc
     2101 cacgctggat aggagttgag atgttatgta atactagata cccttaataa acacatcgaa
     2161 ctcactagga aaagaagtcg acggcttcgc tgggagtgcc caagaaagct accctgccct
     2221 cggccatcag aaggatcttg tcaaagagct caaacagctc ggaagacggc tgatgaatgg
     2281 tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag cacctggacg acgctgtggg
     2341 cggtaaagga gtccagtccg gaggtgggct catcgcagat cagaagcggc ggatcggtta
     2401 gagcctcgga ggcgaatgcc agacgcttcc tttctccgcc ggacagacct ttcaccctgc
     2461 cgggcacacc gatgatcgtg tgctgacatt tgctgagcga aagctcctgg atcacctgat
     2521 ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg catccgcacc atggcttgga
     2581 aaatcaggtg ttccctggcc gttagggagc cgataaagag gtcatcctgc tggacatagg
     2641 cgcacctggc ctgcatctcc ttggcgtcca caggttggcc attgagcagt cgcatcccgg
     2701 atggcgatac ttggatgccc tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct
     2761 ttccggcacc ggaactgccc atcacggcca aaagttcgcc cggataggcc acgccgcaaa
     2821 ctgagtttca aattggtaat tggacccttt attaagattt cacacagatc agccgactgc
     2881 gaatagaaac tcaccgttct tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt
     2941 gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat ccggagcccg gctgattgac
     3001 cgccccaaag atgtccatat tgtgccaggc ataggtgagg ttctcggcta gttggccgct
     3061 ccctgaaccg gagtcctccg gcggactggg tggccggagc gtgccgtagt ttttggcctg
     3121 cccgaagccc tggttaatgc agctctgcga agccgctccg ctgtcaccct gcaatgatag
     3181 gggatctcaa atatcaacta caagcgttat gctcatctaa ccccgaacaa aaagtacccc
     3241 gaagtatcct acgaagtagg tttatacttt tatttatttt ttgtgcatct aggatcagct
     3301 taaaatatct ggttgttata ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt
     3361 agatgtcttg atcatgatat gatctcaaaa attgtcttat atagcgagaa cagctaccag
     3421 aataatctgt ttcgtgtcac tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc
     3481 gcagttctca cgccgcagac aatttgatgt tgcaatcgca gttcctatag atcaagtgaa
     3541 cttaagatgt atgcacatgt actactcaca ttgttcagat gctcggcaga tgggtgtttg
     3601 ctgcctccgc gaattaatag ctcctgatcc tcttggccca ttgccgggat ttttcacact
     3661 ttcccctgct tacccaccca aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa
     3721 ataagaagcg agaggagttt tggcacagca ctttgtgttt aattgatggc gtaaaccgct
     3781 tggagcttcg tcacgaaacc gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt
     3841 aacgctacaa acggtggcga aagagatagc ggacgcagcg gcgaaagaga cggcgatatt
     3901 tctgtggaca gagaaggagg caaacagcgc tgactttgag tggaatgtca ttttgagtga
     3961 gaggtaatcg aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg
     4021 atcagataag ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt tttatctact
     4081 ttccgcaaaa atgggtttta ttaacttaca tacatactag aattggccgc tctagccccc
     4141 cctcgaatgt tctctctctt ctcttctctc tctctttctc gaatgttctc tctcttctct
     4201 tctctctctc tttctcgagg tcatcaagct taggcctcca aggcggagta ctgtcctccg
     4261 ggctggcgga gtactgtcct ccggcaaggc ggagtactgt cctccgggct ggcggagtac
     4321 tgtcctccgg caaggcggag tactgtcctc cgggctggcg gagtactgtc ctccggcaag
     4381 gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaaggcgg agtactgtcc
     4441 tccgggctgg cggagtactg tcctccggca aggcggagta ctgtcctccg ggctggcgga
     4501 gtactgtcct ccggcaaggc ggagtactgt cctccgggct ggcggagtac tgtcctccgg
     4561 caagggtcga gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
     4621 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaaccg gtgatagagc
     4681 ctgaaccaga aaagataaaa gaaggctata ccagtgggag tacacaaaca gagtaagttt
     4741 gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac tgtgcgttag gtcctgttca
     4801 ttggtaccca ccatggtttc caaaggtgaa gaagacaata tggcttccct gcctgcaacc
     4861 cacgagttgc acatttttgg atcgatcaac ggcgtcgatt ttgatatggt aggtcaggga
     4921 acgggaaacc ctaatgacgg ttatgaagaa ctcaatctga agtcgacaaa gggtgatctc
     4981 caattttcgc cttggattct cgtaccgcac attggatatg gatttcatca atatttgcca
     5041 tatccagacg gaatgtcccc gttccaggcg gctatggtcg atggatccgg ttaccaggtc
     5101 cacaggacaa tgcagttcga ggacggcgca tcgctcacgg ttaattaccg ttacacgtac
     5161 gagggtagtc atataaaggg cgaggcacag gtgaaaggaa ccggattccc cgccgatggc
     5221 ccggttatga ccaattcgct cactgcggct gattggtgtc ggtccaaaaa aacatatcct
     5281 aacgacaaaa ctataatatc caccttcaaa tggtcctaca cgaccggaaa tggaaagcgt
     5341 tatcgttcga cggctcggac cacttatact ttcgctaagc ccatggcggc aaactacctc
     5401 aagaatcagc ctatgtatgt attccgaaaa acagagctca agcacagtaa aacggagctg
     5461 aatttcaagg agtggcaaaa agctttcacg gacgttatgg gcatggatga actctacaag
     5521 atggactaca aggatcatga cggtgactac aaagaccatg atatagatta caaggatgac
     5581 gacgacaaga tggatacatt tgacgtttct gataggaaca gctggtactt tggtcccatg
     5641 tctagacagg atgctactga agttttgatg aacgaacgcg agcggggagt gtttttagtc
     5701 cgtgatagta actcgatagc aggggattat gtactttgtg taagagaaga tacaaaagtt
     5761 agcaactaca tcattaacaa agttcaacaa caggatcaaa tcgtttaccg cattggggat
     5821 cagtcttttg acaatctacc gaaactctta actttttaca ctcttcatta tttggataca
     5881 acccctttaa aacggcctgc gtgtagaagg gtggaaaaag taataggaaa gttcgatttc
     5941 gttggcagcg atcaagatga tttacctttt caaagaggtg aagttttaac aatagttcga
     6001 aaagacgagg atcaatggtg gactgcgcgt aactcctcgg ggaaaattgg tcaaataccg
     6061 gttccctata tacaacagta tgacgattat atggatgaag atgctattga taaaaacgaa
     6121 ccttccattt cgggatctag caatgtattt gaaagtactc ttaaaaggac agatttaaat
     6181 cgaaaactac ctgcatacgc ccgcgtaaaa cagtcaaggg tccctaacgc atacgataag
     6241 actgcattaa aattggaaat aggtgacatt attaaagtca ctaaaacaaa cattaatggg
     6301 caatgggagg gagaattaaa tggaaaaaat ggtcattttc ccttcacgca cgttgaattt
     6361 gtcgatgatt gtgatttaag caaaaactcc acagaaatat gctaagcatg catctagagt
     6421 cgacctcgaa cgttaacgtt aacgtaacgt taactcgagg agcttgataa cattatacct
     6481 aaacccatgg tcaagagtaa acatttctgc ctttgaagtt gagaacacaa ttaagcatcc
     6541 cctggttaaa cctgacattc atacttgtta atagcgccat aaacatagca caacctgaca
     6601 ttcatacttg ttaatagcgc cataaacata gcaccaattt cgaagaaatc agttaaaagc
     6661 aattagcaat tagcaattag caataactct gctgacttca aaacgagaag agttgcaagt
     6721 atttgtaagg cacagtttat agaccaccga cggctcatta gggctcgtca tgtaactaag
     6781 cgcggtgaaa cccaattgaa catatagtgg aattattatt atcaatgggg aagatttaac
     6841 cctcaggtag caaagtaatt taattgcaaa tagagagtcc taagactaaa taatatattt
     6901 aaaaatctgg ccctttgacc ttgcttgtca ggtgcatttg ggttcaatcg taagttgctt
     6961 ctatataaac actttcccca tccccgcaat aatgaagaat accgcagaat aaagagagat
     7021 ttgcaacaaa aaataaaggc attgcgaaaa ctttttatgg gggatcatta cactcgggcc
     7081 tacggttaca attcccagcc acttaagcga caagtttggc caacaatcca tctaatagct
     7141 aatagcgcaa tcactggtaa tcgcaagagt atataggcaa tagaacccat ggatttgacc
     7201 aaaggtaacc gagacaatgg agaagcaaga ggatttcaaa ctgaacaccc acagtgctgt
     7261 gtactaccac tggcgcgttt gggagctcac tggcctgatg cgtcctccgg gcgtttcaag
     7321 cctgctttac gtggtatact ccattacggt caacttggtg gtcaccgtgc tgtttccctt
     7381 gagcttgctg gccaggctgc tgttcaccac caacatggcc ggattgtgcg agaacctgac
     7441 cataactatt accgatattg tggccaattt gaagtttgcg aatgtgtaca tggtgaggaa
     7501 gcagctccat gagattcgct ctctcctaag gctcatggac gctagagccc ggctggtggg
     7561 cgatcccgag gagatttctg ccttgaggaa ggaagtgaat atcgcacagg gcactttccg
     7621 cacctttgcc agtattttcg tatttggcac tactttgagt tgcgtccgcg tggtcgttcg
     7681 cccggatcga gagctcctgt atccggcctg gttcggcgtt gactggatgc actccaccag
     7741 aaactatgtg ctcatcaata tctaccagct cttcggcttg atagtgcagg ctatacagaa
     7801 ctgcgctagt gactcctatc cgcctgcgtt tctctgcctg ctcacgggtc atatgcgtgc
     7861 tttggagctg agggtgcggc ggattggcgt cgacgatgta ggtcacggtc tcgaagccgc
     7921 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
     7981 ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca
     8041 ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg
     8101 cgacggcgtc gacgatgtag gtcacggcgg gcagccaagc tttgcgtact cgcaaattat
     8161 taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa ttcaaacccc
     8221 acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac tcaatacgac
     8281 actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag tggatgtctc
     8341 ttgccgacgg gaccacctta tgttatttca tcatggtctg gccattctca tcgtgagctt
     8401 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     8461 tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta
     8521 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     8581 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     8641 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     8701 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     8761 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     8821 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggtgc
     8881 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8941 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     9001 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     9061 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     9121 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     9181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    10021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctt cttcttgaac
    10921 tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc
    10981 aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca
    11041 tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc
    11101 gccgccgatc tgccgctgga ctacgtgggt ctggcccatg atgaaataac ataaggtggt
    11161 cccgtcgata gccgaagctt accgaagtat acacttaaat tcagtgcacg tttgcttgtt
    11221 gagaggaaag gttgtgtgcg gacgaatttt tttttgaaaa cattaaccct tacgtggaat
    11281 aaaaaaaaat gaaatattgc aaattttgct gcaaagctgt gactggagta aaattaattc
    11341 acgtgccgaa gtgtgctatt aagagaaaat tgtgggagca gagccttggg tgcagccttg
    11401 gtgaaaactc ccaaatttgt gatacccact ttaatgattc gcagtggaag gctgcacctg
    11461 caaaaggtca gacatttaaa aggaggcgac tcaacgcaga tgccgtacct agtaaagtga
    11521 tagagcctga accagaaaag ataaaagaag gctataccag tgggagtaca caaacagagt
    11581 aagtttgaat agtaaaaaaa atcatttatg taaacaataa cgtgactgtg cgttaggtcc
    11641 tgttcattgt ttaatgaaaa taagagcttg agggaaaaaa ttcgtacttt ggagtacgaa
    11701 atgcgtcgtt tagagcagca gccgaattaa tt
//
