LOCUS       sequence-258917-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains codon-optimized FnCpf1 and gRNA for streptomyces.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Yeo WL, Heng E, Tan LL, Lim YW, Lim YH, Hoon S, Zhao H, Zhang MM, 
            Wong FT
  TITLE     Characterization of Cas proteins for CRISPR-Cas editing in 
            streptomycetes.
  JOURNAL   Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021.
  PUBMED    31090220
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 May 15. doi: 10.1002/bit.27021."
COMMENT     Sequence Label: pCRISPomyces-FnCpf1
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..302
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica 
                     ribosomal protein S12 gene"
     promoter        4405..4689
                     /label=gapdh promoter
                     /note="glyceraldehyde-3-phosphate dehydrogenase promoter 
                     from Eggerthella lenta"
     primer_bind     4880..4902
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4894..4911
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4895..4911
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4940
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4921..4939
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4987..5007)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4987..5005)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5026..5042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5026..5042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5039..5061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5050..5066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5074..5080,5081..5098,5099..5104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5119..5140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      5199..5233
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(5502..6090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5582..5601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             6294..7094
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(8033..9475)
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            10642..10751
                     /note="incP origin of transfer"
ORIGIN
        1 gccctgcagg cggaagtcag gtagacacga cttccgctag tccttgcaag gtctgctgac
       61 gtgaggcggg gcggtcgttt ttgaccgccc tgccttcgtc atgtaggctc gctcgctgtg
      121 cctggcgtgt catcagacgc ccaggtcccg gtgccgtgag gcccgggcca tcgagccggt
      181 ggtacgtggc tgcggtcccc ttgtgagggc tgcgcgccgt gtgctgtccg gcgcgcacag
      241 ccttgaatcc acccgcgggg gccggccggt ctccgtgagc tcgagtagac gacggagacg
      301 taatgtccat ctaccaggag ttcgtcaaca agtactccct gtccaagacc ctgcgcttcg
      361 agctgatccc gcagggcaag accctggaga acatcaaggc ccgcggcctg atcctggacg
      421 acgagaagcg cgccaaggac tacaagaagg ccaagcagat catcgacaag taccaccagt
      481 tcttcatcga ggagatcctg tcctccgtct gcatctccga ggacctgctc cagaactact
      541 ccgacgtcta cttcaagctg aagaagtccg acgacgacaa cctccagaag gacttcaagt
      601 ccgccaagga caccatcaag aagcagatca gcgagtacat caaggactcc gagaagttca
      661 agaacctgtt caaccagaac ctgatcgacg ccaagaaggg ccaggagtcc gacctgatcc
      721 tgtggctgaa gcagtccaag gacaacggca tcgagctgtt caaggccaac tccgacatca
      781 ccgacatcga cgaggccctg gagatcatca agtccttcaa gggctggacc acctacttca
      841 agggcttcca cgagaaccgc aagaacgtct actcctccaa cgacatcccg acctccatca
      901 tctaccgcat cgtggacgac aacctgccga agttcctgga gaacaaggcc aagtacgagt
      961 ccctgaagga caaggccccg gaggccatca actacgagca gatcaagaag gacctggccg
     1021 aggagctgac cttcgacatc gactacaaga cctccgaggt caaccagcgc gtgttctccc
     1081 tggacgaggt gttcgagatc gccaacttca acaactacct gaaccagtcc ggcatcacca
     1141 agttcaacac catcatcggc ggcaagttcg tcaacggcga gaacaccaag cgcaagggca
     1201 tcaacgagta catcaacctg tactcccagc agatcaacga caagaccctg aagaagtaca
     1261 agatgtccgt cctgttcaag cagatcctgt ccgacaccga gtccaagtcc ttcgtcatcg
     1321 acaagctgga ggacgactcc gacgtcgtca ccaccatgca gtccttctac gagcagatcg
     1381 ccgccttcaa gaccgtcgag gagaagtcca tcaaggagac gctgtccctg ctgttcgacg
     1441 acctgaaggc ccagaagctg gacctgtcca aaatctactt caagaacgac aagtccctga
     1501 ccgacctgtc ccagcaggtg ttcgacgact actccgtcat cggcaccgcc gtcctggagt
     1561 acatcaccca gcagatcgcc ccgaagaacc tggacaaccc gtccaagaag gagcaggagc
     1621 tgatcgccaa gaaaaccgag aaggccaagt acctgtccct ggagacgatc aagctggccc
     1681 tggaggagtt caacaagcac cgcgacatcg acaagcagtg ccgcttcgag gagatcctgg
     1741 ccaacttcgc cgccatcccg atgatcttcg acgagatcgc ccagaacaag gacaacctgg
     1801 cccagatcag catcaagtac cagaaccagg gcaagaagga cctgctccag gcctccgccg
     1861 aggacgacgt caaggccatc aaggacctgc tggaccagac caacaacctg ctgcacaagc
     1921 tgaaaatctt ccacatctcc cagtccgagg acaaggccaa catcctggac aaggacgagc
     1981 acttctacct ggtgttcgag gagtgctact tcgagctggc caacatcgtc ccgctgtaca
     2041 acaagatccg caactacatc acccagaagc cgtactccga cgagaagttc aagctgaact
     2101 tcgagaactc caccctggcc aacggctggg acaagaacaa ggagccggac aacaccgcca
     2161 tcctgttcat caaggacgac aagtactacc tgggcgtcat gaacaagaag aacaacaaaa
     2221 tcttcgacga caaggccatc aaggagaaca agggcgaggg ctacaagaag atcgtctaca
     2281 agctgctgcc gggcgccaac aagatgctgc cgaaggtgtt cttctccgcc aagtccatca
     2341 agttctacaa cccgtccgag gacatcctgc gcatccgcaa ccactccacc cacaccaaga
     2401 acggctcccc gcagaagggc tacgagaagt tcgagttcaa catcgaggac tgccgcaagt
     2461 tcatcgactt ctacaagcag tccatctcca agcacccgga gtggaaggac ttcggcttcc
     2521 gcttctccga cacccagcgc tacaactcca tcgacgagtt ctaccgcgag gtcgagaacc
     2581 agggctacaa gctgaccttc gagaacatct ccgagtccta catcgactcc gtcgtcaacc
     2641 agggcaagct gtacctgttc caaatctaca acaaggactt ctccgcctac tccaagggtc
     2701 gcccgaacct gcacaccctg tactggaagg ccctgttcga cgagcgcaac ctccaggacg
     2761 tcgtctacaa gctgaacggc gaggccgagc tgttctaccg caagcagtcc atcccgaaga
     2821 agatcaccca cccggccaag gaagccatcg ccaacaagaa caaggacaac ccgaagaagg
     2881 agtccgtgtt cgagtacgac ctgatcaagg acaagcgctt caccgaggac aagttcttct
     2941 tccactgccc gatcaccatc aacttcaagt cctccggcgc caacaagttc aacgacgaga
     3001 tcaacctgct gctgaaggag aaggccaacg acgtccacat cctgtcgatc gaccgcggcg
     3061 agcgccacct ggcctactac accctggtgg acggcaaggg caacatcatc aagcaggaca
     3121 ccttcaacat catcggcaac gaccgcatga aaaccaacta ccacgacaag ctggccgcca
     3181 tcgagaagga ccgggactcc gcccgcaagg actggaagaa gatcaacaac atcaaggaga
     3241 tgaaggaagg ctacctgtcc caggtcgtcc acgagatcgc caagctggtc atcgagtaca
     3301 acgccatcgt cgtgttcgag gacctgaact tcggcttcaa gcggggccgc ttcaaggtcg
     3361 agaagcaggt ctaccagaag ctggagaaga tgctgatcga gaagctgaac tacctggtgt
     3421 tcaaggacaa cgagttcgac aagaccggcg gcgtcctgcg ggcctaccag ctgaccgccc
     3481 cgttcgagac gttcaagaag atgggcaagc agaccggcat catctactac gtcccggccg
     3541 gcttcacctc caaaatctgc ccggtcaccg gcttcgtcaa ccagctgtac ccgaagtacg
     3601 agtccgtctc caagtcccag gagttcttct ccaagttcga caaaatctgc tacaacctgg
     3661 acaagggcta cttcgagttc tccttcgact acaagaactt cggcgacaag gccgccaagg
     3721 gcaagtggac catcgcctcc ttcggctccc gcctgatcaa cttccgcaac tccgacaaga
     3781 accacaactg ggacacccgc gaggtctacc cgaccaagga gctggagaag ctgctgaagg
     3841 actactccat cgagtacggc cacggcgagt gcatcaaggc cgccatctgc ggcgagtccg
     3901 acaagaagtt cttcgccaag ctgacctccg tcctgaacac catcctccag atgcgcaact
     3961 ccaagaccgg caccgagctg gactacctga tctccccggt cgccgacgtc aacggcaact
     4021 tcttcgactc ccgccaggcc ccgaagaaca tgccgcagga cgccgacgcc aacggcgcct
     4081 accacatcgg cctgaagggc ctgatgctgc tgggccgcat caagaacaac caggaaggca
     4141 agaagctgaa cctggtcatc aagaacgagg agtacttcga gttcgtccag aaccgcaaca
     4201 actgagaatt cagatctacg cgttccccgc aaaagcggcc tttgactccc tgcaagcctc
     4261 agcgaccgaa tatatcggtt atgcgtgggc gatggttgtt gtcattgtcg gcgcaactat
     4321 cggtatcaag ctgtttaaga aattcacctc gaaagcaagc tgataaaccg atacaattaa
     4381 aggctccttt tggagccttt ttttgctgct ccttcggtcg gacgtgcgtc tacgggcacc
     4441 ttaccgcagc cgtcggctgt gcgacacgga cggatcgggc gaactggccg atgctgggag
     4501 aagcgcgctg ctgtacggcg cgcaccgggt gcggagcccc tcggcgagcg gtgtgaaact
     4561 tctgtgaatg gcctgttcgg ttgctttttt tatacggctg ccagataagg cttgcagcat
     4621 ctgggcggct accgctatga tcggggcgtt cctgcaattc ttagtgcgag tatctgaaag
     4681 gggatacgcg tctaagaact ttaaataatt tctactgttg tagataagtc ttctcagccg
     4741 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gggatcggtg
     4801 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4861 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     4921 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt
     4981 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     5041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5161 cgctttccac cggtgaagac atactccatc tggatttgtt cagaacgctc ggttgccgcc
     5221 gggcgttttt tatctagaag gtaataagaa ctacacgact ggatactgac ttttcacact
     5281 agtcataact cccccagtcc tgcacgctgt cgtattctcc tggccacgac tttacaacac
     5341 cgcacagcat gttgtcaaag cagagaccgt tcgaatgtga acaaagcttc atatgcatgc
     5401 tagctctaga ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5461 ctagaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5761 gacccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6181 atcttcacct agatcctttt ggttcatgtg cagctccatc agcaaaaggg gatgataagt
     6241 ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat
     6301 cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc
     6361 ttctcaacct tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg
     6421 tccggcccct cgaagatggg ccacttggac tgatcgaggc cctgcgtgct gcgctgggtc
     6481 cgggagggac gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg
     6541 ccacgtcgcc cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa
     6601 atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga
     6661 tcatctctga tccattgccc ctgccacctc actcgcctgc aagcccggtc gcccgtgtcc
     6721 atgaactcga tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc
     6781 atcttgccga gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc
     6841 aggatggcaa gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg
     6901 ccttggcgga caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg
     6961 cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc
     7021 gagatccgtt gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc
     7081 gccagtcgat tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg
     7141 cgctgattgc tggggcaaca cgtggagcgg atcggggatt gtctttcttc agctcgctga
     7201 tgatatgctg acgctcaatg ccgtttggcc tccgactaac gaaaatcccg catttggacg
     7261 gctgatccga ttggcacggc ggacggcgaa tggcggagca gacgctcgtc cgggggcaat
     7321 gagatatgaa aaagcctgaa ctcaccgcga cgtatcgatg tcgaggttcc tcaggggagc
     7381 caccccagag aagccctcgg agctgagcgg agctatttcc aaagccatgc cagctagaga
     7441 cagtgcacac cgccaagcat ctgcaaaacc ctcgcctgga gggaaagtgc aatgtacgta
     7501 cgctggtttc cctccagaaa gggattctgc ggcttctcaa ctttggaaga agaggcggaa
     7561 cgaactgctg tttcagccta ctcatgtgag gaggctggtc ctttaccctg atgcccgagg
     7621 gcatcagggt aaaggaccag cctcgccaac taggagcgct accaaggcga acacaaccca
     7681 ggacttggta gaacccttcg ccggagaagt tcagactcat cggcatgagc acccctcgcc
     7741 gcgcactcgg caccggtccg tcgaccacca ccaggacgtc gttgtcgacg tcggccccgc
     7801 ggctcctgcc cgccgaacgc gtcgtcgtcg acggcctggt gctcatcgac gagcacccgg
     7861 agccaggtga aaagcgccgg cggacgctcg gactgggcgc gggattccag cagtaaccca
     7921 ggtccgccgg ccacctcacg gcaggcagac cctcggcttt cgcgccggga ccgccatgag
     7981 accgccaccc ggatgtccgg ggtggcggtc tcatggcggt ccctcagcgg ccctacgcga
     8041 ccgctgtgtc gacgcggagg cagtctccgg ggtgctggcc ccagggctgg agtaccgggg
     8101 cgagcttgcc cttgcagcgg cggcacacgg gcgacgcggc gccgggcggg ggagtcttga
     8161 cgtcgacggt gaccggcttc ggcttcaggc gcttccgaag gctgatcgtc gggcggatcg
     8221 cctccttctt cggctgacgc actcggtcga gcgaagcggc cagctcctcc gtgcgggcct
     8281 cgttggcctt ccggcgcgcc tcgcggaaag cagcttcctc ctcggacatg acctcgaacc
     8341 tcatctggtc agcgtcaagg tcgcccggcg cggccggcgc ttccggggcg ggcgggtcca
     8401 ggacgtcctt gccccacacc aggccccagg actcgacgag ccgccggacg cccggtaggc
     8461 cgtacgtctc ggcgaccttg atgaggtcga ggcgacgtcc ggcgacgcgg gcgatgtatc
     8521 ggtaccagat gtaggccggg atgaccgcga tggcgaccag gccctcggtg tcgtcggtga
     8581 tctcctcctc ggtgcggacg tcctgctgga tgccgagttc cttgatcagc cggttcaggt
     8641 tctgcgaccg gtagtgcttg cggacctgga acacgccgaa ctcgcgctcg cggtacttct
     8701 cgacgaacgg gccgggccga cgaagccgct gcagctcggc ggccgccgcg tcgcccaggt
     8761 cgagcggtcc catgcggtcg tcgccgcgac cggccttgaa gttctgtccg gccagctcca
     8821 ggccgatctt ggcgacgccg cccttggtct tgtcgccgtc cttgtagagg tagcgggcct
     8881 gcttgcccgc atcgccgtca gcggcgtccg cgccgttgag tgggcgcacg tcggtgccgt
     8941 ggcccttgcc ctcacaggag caaccgggcc ggtcgcacgt ctcgctgacg gtgtagccgc
     9001 ccgcggattc gaccccggcg gcccaggctc cggcgagtgc gtcgcggaac gcggcctggg
     9061 cgtccgggcc gagcacctcg cgggtgaccc agagcgtgtg ccagtgcagg tgccagccgg
     9121 agccccagcc gaaggtgtcc tcgaaggccc gctcgtagcc gatgatcccg aagtcgtcgc
     9181 gcatcgtgcg ccagcggcgg ccggacgagc cgtacgcgcc cttccagccg tcgtgcaaga
     9241 ccgcgaccag gccgtgccgc attcccttgc ggacggtgcc gaacgccatg cgctcgaagt
     9301 ggcgcaacgt gttcgtgcca aggtgcagcc cgtacccggc gtccgcgaga ccgtcggcgg
     9361 cgagctgcac gttcgagccc cgtacggcca ggatgcggct catgcaccac gggcaggtgt
     9421 ggacgttgtt gcagcggcac gtgttgcccc acgtcgcctc gcccggcttc cacatcagct
     9481 cggccgtccc ggcagtgagc cgggtcccgc agcccttgaa cgcctcgttc agcgacaccg
     9541 tctggtgccg gtcccgccgg gcgaaccgct cgtcgcgcgg gtcctcccgc ccggctgtcg
     9601 cggcaccctc gtttggggta gaacccgttc cagttacagc gctctgacct gcagtggacg
     9661 gagattttcc cttactacta aagcccgcgt ccggattacc cgctgtagtc gtgcttgcta
     9721 cgctgcgtga ctggtccgca atgagacgct ttgcgcgctt tcggcaggcg tccgagcagt
     9781 agattttggg gcgcttcccg gggatgtgga cgatcggggt gccgcagtgg cacttcggtc
     9841 cggcggggcg cggtggtgtc gacgcgctgt tctctcgtac gctcgtcaca gagcaaacgt
     9901 cctcactcgg catgctgcgc cggttcgggg gcggcgagcc cgggaggcca atcccgggct
     9961 cgtgccattt ctgggtcctg ttgatcctgg cattggtgtg gccgttcatt gcccctgctc
    10021 gctcctgacg cgccgataga cgtccgatac gcccggtgct ggtgggattt gataggtcgg
    10081 aagaagcccc gcccgggcct gggcggggct tcctgtgcgt caggacctcc tcgtcgtgag
    10141 cctcttcggc ctatggacgg agtgacctcg tgatccgtta cagccgcgcg cgctcgcgta
    10201 gagcggtctc atcagttcca cgaacggtcc tcttcgcaga tcagggcgtt ggggcggagt
    10261 ctcaccaagg actacgtctg ctggcgattt ccgttacacc ccgggcggtg gccggcgcac
    10321 acgcgcgccc gcgttgggca gtgcagaaag tgcagaaacc taggcgctga tggtccaggt
    10381 ccacggttcg tcgtcggcgg cggcgcgggc ggcggcgtcg gccagggcgc gggcgagacc
    10441 ggctacggcg ggcttgatgc gccggttgcg ggcgaccttg agcagctagt atgcaggtcg
    10501 acggatcttt tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata
    10561 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
    10621 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
    10681 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
    10741 aggctggccg gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa
    10801 gccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc
    10861 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg
    10921 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tg
//
