LOCUS       sequence-258920-        6812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRICK translation biosensor with 6x copies of modified PP7 stem 
            loops and 24x MS2 stem loops. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pmax ponA 6xTRICK 24xMS2SL
FEATURES             Location/Qualifiers
     source          1..6812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1793..2031
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1978..1999
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          2081..2213
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             2321..2347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2354..2380
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2387..2413
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4986..5005)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5015..5096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5040..5059
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5294..5882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5374..5393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5963..6757)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5963..6757)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6074..6093)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6684..6703
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ggacagcaga gatccagttt ggactagtag atctcggccg catattaagt gcattgttct
       61 cgataccgct aagtgcattg ttctcgttag ctcgatggac aagtgcattg ttctcttgct
      121 gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg
      181 ttctcttgct gaaagctcag tacccgggtc ggagtacgga tctcggccgc atattaagtg
      241 cattgttctc gataccgcta agtgcattgt tctcgttagc tcgatggaca agtgcattgt
      301 tctcttgctg aaagctcgat ggacaagtgc attgttctct tgctgaaagc tcgatggaca
      361 agtgcattgt tctcttgctg aaagctcagt acccgggtcg gagtacggat ctcggccgca
      421 tattaagtgc attgttctcg ataccgctaa gtgcattgtt ctcgttagct cgatggacaa
      481 gtgcattgtt ctcttgctga aagctcgatg gacaagtgca ttgttctctt gctgaaagct
      541 cgatggacaa gtgcattgtt ctcttgctga aagctcagta cccgggtcgg agtacggatc
      601 tcggccgcat attaagtgca ttgttctcga taccgctaag tgcattgttc tcgttagctc
      661 gatggacaag tgcattgttc tcttgctgaa agctcgatgg acaagtgcat tgttctcttg
      721 ctgaaagctc gatggacaag tgcattgttc tcttgctgaa agctcagtac ccgggtcgga
      781 gtacggatct cggccgcata ttaagtgcat tgttctcgat accgctaagt gcattgttct
      841 cgttagctcg atggacaagt gcattgttct cttgctgaaa gctcgatgga caagtgcatt
      901 gttctcttgc tgaaagctcg atggacaagt gcattgttct cttgctgaaa gctcagtacc
      961 cgggtcggag tacggatctc ggccgcatat taagtgcatt gttctcgata ccgctaagtg
     1021 cattgttctc gttagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac
     1081 aagtgcattg ttctcttgct gaaagctcga tggacaagtg cattgttctc ttgctgaaag
     1141 ctcagtaccc gggtcggagt acggatctcg gccgcatatt aagtgcattg ttctcgatac
     1201 cgctaagtgc attgttctcg ttagctcgat ggacaagtgc attgttctct tgctgaaagc
     1261 tcgatggaca agtgcattgt tctcttgctg aaagctcgat ggacaagtgc attgttctct
     1321 tgctgaaagc tcagtacccg ggtcggagta cggatctcgg ccgcatatta agtgcattgt
     1381 tctcgatacc gctaagtgca ttgttctcgt tagctcgatg gacaagtgca ttgttctctt
     1441 gctgaaagct cgatggacaa gtgcattgtt ctcttgctga aagctcgatg gacaagtgca
     1501 ttgttctctt gctgaaagct cagtacccgg gtcggagtac ggatctcggc cgcatattaa
     1561 gtgcattgtt ctcgataccg ctaagtgcat tgttctcgtt agctcgatgg acaagtgcat
     1621 tgttctcttg ctgaaagctc gatggacaag tgcattgttc tcttgctgaa agctcgatgg
     1681 acaagtgcat tgttctcttg ctgaaagctc agtacccggg tcggagtact gccccgcccc
     1741 tagcgattag ccccggcccc gcatagctcc gccccgggag taccctcgac cgccggagta
     1801 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     1861 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     1921 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     1981 actactgaaa tctgccaaga agtaattatt gaatacaaga agacaactct gaatactttc
     2041 aaaagttacc gagaaagaag aactcagaca cggatcacag gtaagtatca aggttacaag
     2101 acaggtttaa ggaggccaat agaaactggg cttgtcgaga cagagaagat tcttgcgttt
     2161 ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca caggggtacc
     2221 gaagccgcta gcgctaccgg tcgccaccat gtcgcggagg cggcacagct acgagaacga
     2281 tggtggacaa cctcacaaaa ggaggaagac gtctgttgag tacccttatg atgtccctga
     2341 ctacgctgcc gagtatccct acgacgtccc cgattatgcc gcagaatacc catacgatgt
     2401 gccagactac gcagctgagg gggggcaagt ccagttgcaa gaatctggac ctggtttggt
     2461 taaaccatct gagacacttt ccctaacctg cacagtcagt ggcgcatcaa tttcctcctc
     2521 gcactattat tggggttgga ttagacaacc acctggaaag ggcccagagt ggatagggtc
     2581 tatgtactac tcgggtagaa cttactataa tcctgccttg aagtcacgtg ttaccatatc
     2641 ctctgataag tcaaaaaacc aattcttttt aaaactcact agcgttaccg ctgctgatac
     2701 agctgtctat tatgctgccc gtgagggccc aacgcattat tatgataatt caggcccaat
     2761 acctagcgat gagtattttc aacattgggg ccagggcact ttagttacag tttccggtgg
     2821 tggcggctct ggtggcggtg gcagcggcgg tggtggttcc ggaggcggcg gttctcaggt
     2881 gcagctgcag gagtcgggcc caggactggt gaagccttcg gagaccctgt ccctcacctg
     2941 cactgtctct ggtgcctcca tcagcagtag tcattactac tggggctgga ttcgccagcc
     3001 cccagggaag gggcctgagt ggattgggag tatgtattat agtgggagaa cgtactacaa
     3061 cccggccctc aagagtcgag tcaccatatc atcagacaag tcgaagaacc agttcttctt
     3121 gaagttgacc tctgtaaccg ccgcggacac ggccgtgtat tacgctgcga gggagggacc
     3181 cacacattac tatgataata gtggtccaat accttcggat gagtatttcc agcactgggg
     3241 ccagggtacc ctggtcactg tctccgtcga cagatcctac ctgcaaagca ccagaggata
     3301 tggcctcggt gcctcagcat cttcagatgt gctgtcactg tccaatcttc aagccagcag
     3361 agcatatggg ctcgctggct ccactgtcat tacagtggac ctgatcttct ccaatcaaag
     3421 gagcagagga tatggcctcg ctccctccga ctacatcagg ctggaagtga catccatcat
     3481 tcatcccagc agagcatatg ggctcgctgg gaccacagca tgcctcgaag tgctgagaga
     3541 ctatcatcaa accgacagag gatatggcct cgtcggctcc tactccctcc tggtggaata
     3601 cttctccact cttcaaccca gcagagcata tgggctcgct gggtccatca gagatacaag
     3661 atctctcgag tgataaggat ctcggatcct acggtactta ttgccaagaa agcacgagca
     3721 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     3781 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     3841 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     3901 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     3961 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tcctacggta
     4021 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     4081 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     4141 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     4201 gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa agcacgagca
     4261 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     4321 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     4381 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     4441 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     4501 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tcctacggta
     4561 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     4621 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     4681 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     4741 gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa agcacgagca
     4801 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     4861 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     4921 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     4981 gatccgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     5041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     5101 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     5161 gcaagtaaaa cctctacaaa tgtggtactt aagaggggga gaccaaaggg cgagacgtta
     5221 aggcctcacg tgacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5281 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5341 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5401 gctccctcgt gcgctctcct gttccgaccc tgccgcttac gggatacctg tccgcctttc
     5461 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5521 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5581 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5641 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5701 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5761 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5821 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5881 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5941 aagggatttt ggtcatgccg tctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     6001 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     6061 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     6121 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     6181 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     6241 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     6301 caagaccggc ttccatccga gtacgtgctc tctcgatgcg atgtttcgct tggtggtcga
     6361 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     6421 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     6481 gcagccagtc ccttcccgct tcagtgacaa cgtcgagtac agctgcgcaa ggaacgcccg
     6541 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     6601 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     6661 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     6721 ccggagaacc tgcgtgcaat ccatcttgtt caatcataat attattgaag catttatcag
     6781 ggttcgtctc gtcccggtct cctcccatgc at
//
