LOCUS       sequence-258932-        3816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRDV5 plasmid containing a convex Sybody for the generation of a 
            qPCR standard curve.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Brauer P, Newstead S, 
            Dawson RJP, Geertsma ER, Seeger MA
  TITLE     Generation of synthetic nanobodies against delicate proteins.
  JOURNAL   Nat Protoc. 2020 Apr 8. pii: 10.1038/s41596-020-0304-x. doi: 
            10.1038/s41596-020-0304-x.
  PUBMED    32269381
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Apr 8. pii: 10.1038/s41596-020-0304-x. doi: 
            10.1038/s41596-020-0304-x."
COMMENT     Sequence Label: pRDV5 containing Sb-convex
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             69..91
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1454..1472)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1468..1515
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1551..2006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1638..1657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1848..1869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2033..2137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2138..2206,2207..2998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2356..2375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3169..3756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3657..3676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acccgacacc atcgaaatta atacgactca ctatagggag accacaacgg tttccctcta
       61 gaaataattt tgtttaactt taagaaggag atatatccat gggtagtcaa gtccagctgg
      121 tggaatcggg tggtggtagc gtccaggcgg gtggtagcct gcgtctgagc tgtgcggcta
      181 gcggctctat ttccagcatc acgtacctgg gctggtttcg ccaggcaccg ggcaaagagc
      241 gtgagggcgt cgcagcgctg agcaccagct ccggtaccac ctactacgcg gacagcgtta
      301 agggtcgttt cacggtgagc ctggacaacg ccaagaatac cgtgtatctg caaatgaaca
      361 gcttgaaacc ggaagatact gctttgtatt actgcgcggc agccagcagc ggctccagca
      421 gcccgctgtc tagcagcagc tatacgtact ggggtcaggg cacccaagtt accgtttctg
      481 caaagcttta tatggcctcg ggggccgaat tcggatctgg tggccagaag caagctgaag
      541 aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcagat gctaaagctg
      601 cggaagaagc agcgaagaaa gcggctgcag acgcaaagaa aaaagcagaa gcagaagccg
      661 ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc cgctgcggca ctgaagaaga
      721 aagcggaagc ggcagaagca gctgcagctg aagcaagaaa gaaagcggca actgaagctg
      781 ctgaaaaagc caaagcagaa gctgagaaga aagcggctgc tgaaaaggct gcagctgata
      841 agaaagcggc agcagagaaa gctgcagccg acaaaaaagc agcagaaaaa gcggctgctg
      901 aaaaggcagc agctgataag aaagcagcgg cagaaaaagc cgccgcagac aaaaaagcgg
      961 cagcggcaaa agctgcagct gaaaaagccg ctgcagcaaa agcggccgca gaggcagatg
     1021 atattttcgg tgagctaagc tctggtaaga atgcaccgaa aacgggggga ggggcgaaag
     1081 ggaacaatgc ttcgcctgcc gggagtggta atactaaaaa caatggcgca tcaggggccg
     1141 atatcaataa ctatgccggg cagattaaat ctgctatcga aagtaagttc tatgacgcat
     1201 cgtcctatgc aggcaaaacc tgtacgctgc gcataaaact ggcacccgat ggtatgttac
     1261 tggatatcaa acctgaaggt ggcgatcccg cactttgtca ggctgcgttg gcagcagcta
     1321 aacttgcgaa gataccgaaa ccaccaagcc aggcagtata tgaagtgttc aaaaacgcgc
     1381 cattggactt caaaccgtag tagagatccg gctgctaaca aagcccgaaa ggaagctgag
     1441 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1501 ttgaggggtt ttttgctgaa aggaggaact atatccggat cgagatcccc acgcgccctg
     1561 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1621 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1681 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1741 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1801 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1861 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1921 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1981 taacaaaata ttaacgctta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     2041 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2101 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2161 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2221 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2281 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2341 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2401 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2461 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2521 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2581 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2641 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2701 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2761 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2821 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2881 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2941 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3001 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3061 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3301 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctctgc cagtggcgat
     3421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3781 cggttcctgg ccttttgctg gccttttgct cacatg
//
