LOCUS       sequence-258943-        4677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO2B1
FEATURES             Location/Qualifiers
     source          1..4677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2802..2901)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2918..2937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2919..2937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2942..2958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2942..2958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3060..3079)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3071..3880
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3138..3157)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4027..4615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4516..4535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcc caaggatcgg accagcagga gaggtgcctc aggtgccaga
      721 caaggagacc aaggccacaa tgggaaccga gaacacacca ggaggcaagg caagcccaga
      781 ccctcaggat gtgcgccctt ccgtgttcca caatatcaag ctgtttgtgc tgtgccacag
      841 cctgctgcag ctggcccagc tgatgatctc tggctacctg aagagctcca tcagcaccgt
      901 ggagaagagg ttcggcctgt ctagccagac atctggcctg ctggccagct ttaacgaagt
      961 gggcaatacc gccctgatcg tgttcgtgtc ttactttggc agccgcgtgc accggccaag
     1021 aatgatcggc tatggagcaa tcctggtggc cctggcagga ctgctgatga ccctgcctca
     1081 cttcatctct gagccatacc ggtatgataa cacaagccct gaggacatgc cacaggactt
     1141 caaggccagc ctgtgcctgc caaccacatc cgccccagca tctgccccta gcaacggaaa
     1201 ttgttcctct tataccgaga cacagcacct gtccgtggtg ggcatcatgt tcgtggcaca
     1261 gaccctgctg ggagtgggag gcgtgcccat ccagcctttc ggcatcagct acatcgacga
     1321 ttttgcccac aactctaata gcccactgta tctgggcatc ctgttcgcag tgaccatgat
     1381 gggaccagga ctggcctttg gcctgggatc tctgatgctg cggctgtacg tggacatcaa
     1441 tcagatgcct gagggcggca tcagcctgac aatcaaggat ccaagatggg tgggagcatg
     1501 gtggctgggc ttcctgatcg cagcaggagc agtggccctg gcagccatcc catatttctt
     1561 tttcccaaag gagatgccca aggagaagcg ggagctgcag tttcggagaa aggtgctggc
     1621 agtgaccgac tccccagcaa gaaagggcaa ggattcccca tctaagcagt cccccggcga
     1681 gtctaccaag aagcaggacg gcctggtgca gatcgcccct aacctgacag tgatccagtt
     1741 catcaaggtg tttccaaggg tgctgctgca gaccctgagg cacccaatct tcctgctggt
     1801 ggtgctgagc caggtgtgcc tgagctccat ggcagcagga atggccatct tcctgcccaa
     1861 gtttctggag agacagttca gcatcacagc ctcctacgcc aatctgctga tcggctgtct
     1921 gtcctttcca tctgtgatcg tgggaatcgt ggtgggaggc gtgctggtga agaggctgca
     1981 cctgggacca gtgggatgcg gcgccctgtg cctgctgggc atgctgctgt gcctgttttt
     2041 ctccctgcct ctgtttttca tcggctgttc tagccaccag atcgccggca tcacccacca
     2101 gacatctgcc cacccaggac tggagctgag cccttcctgc atggaggcct gcagctgtcc
     2161 cctggacggc ttcaaccccg tgtgcgatcc atccaccaga gtggagtaca tcacaccttg
     2221 ccacgccggc tgttcctctt gggtggtgca ggacgccctg gataactccc aggtgtttta
     2281 taccaattgc tcttgcgtgg tggagggcaa tcctgtgctg gccggcagct gcgactccac
     2341 atgttctcac ctggtggtgc cattcctgct gctggtgagc ctgggatccg ccctggcatg
     2401 cctgacccac acaccttcct ttatgctgat cctgaggggc gtgaagaagg aggataagac
     2461 cctggccgtg ggcatccagt tcatgtttct gcgcatcctg gcctggatgc catcccccgt
     2521 gatccacggc tctgccatcg acaccacatg cgtgcactgg gcactgagct gcggcaggcg
     2581 cgccgtgtgc cgctactata acaatgatct gctgaggaac cgcttcatcg gcctgcagtt
     2641 tttctttaag accggctccg tgatctgttt tgcactggtg ctggccgtgc tgaggcagca
     2701 ggacaaggag gcaaggacaa aggagagcag aagctcccca gcagtggagc agcagctgct
     2761 ggtgtccgga ccaggcaaga agcctgagga ttctagagtg gacccagctt tcttgtacaa
     2821 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     2881 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     2941 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     3001 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     3061 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     3121 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     3181 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     3241 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     3301 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     3361 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     3421 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     3481 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     3541 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     3601 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     3661 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     3721 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     3781 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     3841 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     3901 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     3961 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
