LOCUS       sequence-25896-e         497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for NTAP (N-terminal affinity purification), 
            frame A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-NTAP-A (w322-1)
FEATURES             Location/Qualifiers
     source          1..497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      221..307
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      399..426
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     434..457
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgctagcatg gatctcgggg
      181 acgtctaact actaagcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
      241 gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      301 gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc
      361 aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg
      421 cctttttgcg tttctacaaa ctcttcctgt tagttagtta cttaagctcg ggccccaaat
      481 aatgatttta ttttgac
//
