LOCUS       sequence-25899-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-V5-2-Ubc9 (w345-2)
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    75..174
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag
       61 ctgcagctgg atggcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      121 aaattgataa gcaatgcttt cttataatgc caactttgta caagaaagct gggtctagca
      181 caaggtcgct gcttatgagg gcgcaaactt cttggcttgt gctcggaccc ttttctcgta
      241 ctccactctg ttttggcagt aaatcgtgta ggcctctgct tgagctgggt cttggatatt
      301 tggttcattt agaagttcct gtattcctaa taggatctgt ttgattgtga tggctggcct
      361 ccagtccttg tcctcctcta agatggacag gcacactgtc cccgaagggt acacattcgg
      421 gtgaaataat ggtggttcga atttacattt tggtggcgaa gatggataat catctttgaa
      481 aagcatccgt agtttaaaca agcctccttc ccacggagtc cctttctttc ctggaatggc
      541 gcactcccag ttcatgaggt tcatcgtgcc atcgggattt tttgttggga cagccacgaa
      601 accaaatggg tggtctttcc tccatgcttt cctctcctgg gcgagtctgc tgagggcgat
      661 ccccgacatg ccaccagagc c
//
