LOCUS       sequence-258998-       13322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replication helper.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
  TITLE     A Novel Ternary Vector System United with Morphogenic Genes Enhances
            CRISPR/Cas Delivery in Maize.
  JOURNAL   Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 
            10.1104/pp.19.00767.
  PUBMED    31558579
REFERENCE   2  (bases 1 to 13322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767."
COMMENT     Sequence Label: pVS1-SAH2
FEATURES             Location/Qualifiers
     source          1..13322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4148..5296)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             8348..8977
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9406..10479
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10545..10739
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10975..10997)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11083..11223
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11138..11157
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11238..11255)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11409..11997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11489..11508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12253..13044)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtgcgcagca gctcgttatc gaccggctgc ggcgtcgggg ccgccgcctt gcgctgcggt
       61 cggtgttcct tcttcggctc gcgcagcttg aacagcatga tcgcggaaac cagcagcaac
      121 gccgcgccta cgcctcccgc gatgtagaac agcatcggat tcattcttcg gtcctccttg
      181 tagcggaacc gttgtctgtg cggcgcgggt ggcccgcgcc gctgtctttg gggatcagcc
      241 ctcgatgagc gcgaccagtt tcacgtcggc aaggttcgcc tcgaactcct ggccgtcgtc
      301 ctcgtacttc aaccaggcat agccttccgc cggcggccga cggttgagga taaggcgggc
      361 agggcgctcg tcgtgctcga cctggacgat ggcctttttc agcttgtccg ggtccggctc
      421 cttcgcgccc ttttccttgg cgtccttacc gtcctggtcg ccgtcctcgc cgtcctggcc
      481 gtcgccggcc tccgcgtcac gctcggcatc agtctggccg ttgaaggcat cgacggtgtt
      541 gggatcgcgg cccttctcgt ccaggaactc gcgcagcagc ttgaccgtgc cgcgcgtgat
      601 ttcctgggtg tcgtcgtcaa gccacgcctc gacttcctcc gggcgcttct tgaaggccgt
      661 caccagctcg ttcaccacgg tcacgtcgcg cacgcggccg gtgttgaacg catcggcgat
      721 cttctccggc aggtccagca gcgtgacgtg ctgggtgatg aacgccggcg acttgccgat
      781 ttccttggcg atatcgcctt tcttcttgcc cttcgccagc tcgcggccaa tgaagtcggc
      841 aatttcgcgc ggggtcagct cgttgcgttg caggttctcg ataacctggt cggcttcgtt
      901 gtagtcgttg tcgatgaacg ccgggatgga cttcttgccg gcccacttcg agccacggta
      961 gcggcgggcg ccgtgattga tgatatagcg gcccggctgc tcctggttct cgcgcaccga
     1021 aatgggtgac ttcaccccgc gctctttgat cgtggcaccg atttccgcga tgctctccgg
     1081 ggaaaagccg gggttgtcgg ccgtccgcgg ctgatgcgga tcttcgtcga tcaggtccag
     1141 gtccagctcg atagggccgg aaccgccctg agacgccgca ggagcgtcca ggaggctcga
     1201 caggtcgccg atgctatcca accccaggcc ggacggctgc gccgcgcctg cggcttcctg
     1261 agcggccgca gcggtgtttt tcttggtggt cttggcttga gccgcagtca ttgggaaatc
     1321 tccatcttcg tgaacacgta atcagccagg gcgcgaacct ctttcgatgc cttgcgcgcg
     1381 gccgttttct tgatcttcca gaccggcaca ccggatgcga gggcatcggc gatgctgctg
     1441 cgcaggccaa cggtggccgg aatcatcatc ttggggtacg cggccagcag ctcggcttgg
     1501 tggcgcgcgt ggcgcggatt ccgcgcatcg accttgctgg gcaccatgcc aaggaattgc
     1561 agcttggcgt tcttctggcg cacgttcgca atggtcgtga ccatcttctt gatgccctgg
     1621 atgctgtacg cctcaagctc gatgggggac agcacatagt cggccgcgaa gagggcggcc
     1681 gccaggccga cgccaagggt cggggccgtg tcgatcaggc acacgtcgaa gccttggttc
     1741 gccagggcct tgatgttcgc cccgaacagc tcgcgggcgt cgtccagcga cagccgttcg
     1801 gcgttcgcca gtaccgggtt ggactcgatg agggcgaggc gcgcggcctg gccgtcgccg
     1861 gctgcgggtg cggtttcggt ccagccgccg gcagggacag cgccgaacag cttgcttgca
     1921 tgcaggccgg tagcaaagtc cttgagcgtg taggacgcat tgccctgggg gtccaggtcg
     1981 atcacggcaa cccgcaagcc gcgctcgaaa aagtcgaagg caagatgcac aagggtcgaa
     2041 gtcttgccga cgccgccttt ctggttggcc gtgaccaaag ttttcatcgt ttggtttcct
     2101 gttttttctt ggcgtccgct tcccacttcc ggacgatgta cgcctgatgt tccggcagaa
     2161 ccgccgttac ccgcgcgtac ccctcgggca agttcttgtc ctcgaacgcg gcccacacgc
     2221 gatgcaccgc ttgcgacact gcgcccctgg tcagtcccag cgacgttgcg aacgtcgcct
     2281 gtggcttccc atcgactaag acgccccgcg ctatctcgat ggtctgctgc cccacttcca
     2341 gcccctggat cgcctcctgg aactggcttt cggtaagccg tttcttcatg gataacaccc
     2401 ataatttgct ccgcgccttg gttgaacata gcggtgacag ccgccagcac atgagagaag
     2461 tttagctaaa catttctcgc acgtcaacac ctttagccgc taaaactcgt ccttggcgta
     2521 acaaaacaaa agcccggaaa ccgggctttc gtctcttgcc gcttatggct ctgcacccgg
     2581 ctccatcacc aacaggtcgc gcacgcgctt cactcggttg cggatcgaca ctgccagccc
     2641 aacaaagccg gttgccgccg ccgccaggat cgcgccgatg atgccggcca caccggccat
     2701 cgcccaccag gtcgccgcct tccggttcca ttcctgctgg tactgcttcg caatgctgga
     2761 cctcggctca ccataggctg accgctcgat ggcgtatgcc gcttctcccc ttggcgtaaa
     2821 acccagcgcc gcaggcggca ttgccatgct gcccgccgct ttcccgacca cgacgcgcgc
     2881 accaggcttg cggtccagac cttcggccac ggcgagctgc gcaaggacat aatcagccgc
     2941 cgacttggct ccacgcgcct cgatcagctc ttgcactcgc gcgaaatcct tggcctccac
     3001 ggccgccatg aatcgcgcac gcggcgaagg ctccgcaggg ccggcgtcgt gatcgccgcc
     3061 gagaatgccc ttcaccaagt tcgacgacac gaaaatcatg ctgacggcta tcaccatcat
     3121 gcagtctaga taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
     3181 ggttccttgt aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa
     3241 gtcctccggc cgccagttgc caggccgtaa aggtgagcag aggcacggga ggttgccact
     3301 tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc
     3361 cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc
     3421 aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga
     3481 acacgaccat cagcggctgc acagcgccta ccgtcgccgc gacccgcccg gcaggcggta
     3541 gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat
     3601 gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc
     3661 cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa
     3721 aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga
     3781 cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc
     3841 agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg
     3901 agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag
     3961 ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta tcggcagttc
     4021 gtagagcgcg ccgtgcgccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg
     4081 cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca
     4141 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     4201 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     4261 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     4321 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     4381 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     4441 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     4501 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     4561 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     4621 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     4681 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     4741 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     4801 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     4861 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     4921 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
     4981 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     5041 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     5101 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
     5161 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     5221 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     5281 caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt
     5341 gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg
     5401 caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct
     5461 tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag
     5521 agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc
     5581 gccactcttc attaaccgct atatcgaaaa ttgcttgcgg cttgttagaa ttgccatgac
     5641 gtacctcggt gtcacgggta agattaccga taaactggaa ctgattatgg ctcatatcga
     5701 aagtctcctt gagaaaggag actctagttt agctaaacat tggttccgct gtcaagaact
     5761 ttagcggcta aaattttgcg ggccgcgacc aaaggtgcga ggggcggctt ccgctgtgta
     5821 caaccagata tttttcacca acatccttcg tctgctcgat gagcggggca tgacgaaaca
     5881 tgagctgtcg gagagggcag gggtttcaat ttcgttttta tcagacttaa ccaacggtaa
     5941 ggccaacccc tcgttgaagg tgatggaggc cattgccgac gccctggaaa ctcccctacc
     6001 tcttctcctg gagtccaccg accttgaccg cgaggcactc gcggagattg cgggtcatcc
     6061 tttcaagagc agcgtgccgc ccggatacga acgcatcagt gtggttttgc cgtcacataa
     6121 ggcgtttatc gtaaagaaat ggggcgacga cacccgaaaa aagctgcgtg gaaggctctg
     6181 acgccaaggg ttagggcttg cacttccttc tttagccgct aaaacggccc cttctctgcg
     6241 ggccgtcggc tcgcgcatca tatcgacatc ctcaacggaa gccgtgccgc gaatggcatc
     6301 gggcgggtgc gctttgacag ttgttttcta tcagaacccc tacgtcgtgc ggttcgatta
     6361 agcttggacc taaagtcgtt aattaaagta cgccatcagg acgttgtgag tggcgcgatt
     6421 tttagcggct gaaatcagcc cttgagcctg tcggcaagtc gcgtcatgag gtccatgcgc
     6481 tcatgcagga tcgccacgac caacgcgggt tcgcccgcac gcggcaggca aaaaacgtag
     6541 tggtgttcgc agcgggccat ccgcagcgcg ggaaagagtt cgctcatgtc cttaaacggg
     6601 ccttcgccgg cggcaagcct ggctatgccc tgttccagct tagcgatata gcggcgcacc
     6661 tgcgccgcgc cccactcccg gcgcgtgtag cggatgatgc cgcgtagatc ggcttcggcc
     6721 tcagccgtga ggatgtaggc cgtcaagcgc gatccccgct gagttcttca tcaagaattt
     6781 cgccgacgct cttggtggac accttgccgg caagcccatc gttgatgcgg ttccccagca
     6841 tggttttcag ttcctgccat gcctgatcgg catcagcgtc accggggaac agacgttcga
     6901 gggcgtattg cttaatggtc ttgccctgca aggcggccag ggctttcagg ctctggtgct
     6961 gctggtccgt catgtcgatt gtcaggcggc tcattggata acctccataa aatacacgta
     7021 accacattag cacatatgtg ggcgtgaggc tacagcgcga ggcgcattaa ggtcgggaaa
     7081 atgcgctagg cgcatttaaa ttgcgtattg ctgtaatgcg ccatgccggc tagactaggc
     7141 ccaaatgggt atacccaatt tgaccaaggg ggacgcgatg aattaagcta tagtgcagtc
     7201 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     7261 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     7321 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     7381 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     7441 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     7501 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     7561 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     7621 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     7681 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     7741 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     7801 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     7861 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     7921 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     7981 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     8041 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     8101 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     8161 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     8221 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     8281 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     8341 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     8401 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     8461 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     8521 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     8581 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     8641 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     8701 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     8761 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     8821 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     8881 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     8941 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     9001 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     9061 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     9121 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     9181 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     9241 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     9301 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     9361 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
     9421 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     9481 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     9541 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     9601 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     9661 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     9721 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     9781 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     9841 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     9901 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     9961 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
    10021 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
    10081 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
    10141 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
    10201 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
    10261 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
    10321 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
    10381 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
    10441 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
    10501 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
    10561 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
    10621 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
    10681 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
    10741 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
    10801 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
    10861 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
    10921 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
    10981 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    11041 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
    11101 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
    11161 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    11221 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11281 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11821 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    11881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12061 attttggtca tgcatgatat atctcccaat ttgtgtaggc gcgccttggg cttattatgc
    12121 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
    12181 ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
    12241 tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
    12301 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
    12361 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
    12421 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
    12481 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
    12541 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
    12601 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
    12661 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
    12721 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
    12781 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
    12841 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
    12901 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
    12961 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
    13021 ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg
    13081 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
    13141 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
    13201 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
    13261 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgaat
    13321 tc
//
