LOCUS       sequence-259-exp        3489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4417
FEATURES             Location/Qualifiers
     source          1..3489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     98..114
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     98..114
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(124..837)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(164..184)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     779..807
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(862..880)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(890..907)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(890..906)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(899..921)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1048..1503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1135..1154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1345..1366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1529..1633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1634..1702,1703..2494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1852..1871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2665..3253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3154..3173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3407..3424
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacctgg cttatcgaaa ttaatacgac tcactatagg gagaccggca gatctgatat
       61 catcgatgaa ttcgagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg
      121 gcatttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
      181 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      241 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      301 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc
      361 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      421 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      481 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      541 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      601 tttcatatga tctgggtatc ttgaaaagca ttgaacacca taagagaaag tagtgacaag
      661 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc
      721 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
      781 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcatttt
      841 ttctaccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
      901 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      961 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1021 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1201 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1261 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1441 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1501 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3361 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     3421 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3481 ttaatgcag
//
