LOCUS       sequence-25900-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Degron, C-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-FKBP12DD C-term (w350-2)
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(194..514)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     protein_bind    complement(581..680)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttattgttc ntgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
       61 acgggccaga gctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc
      181 tgggtctagc tcattccggt tttagaagct ccacatcgaa gacgagagtg gcatgtggtg
      241 ggatgatgcc tgggtgccca gtggcaccat aggcataatc tggagatata gtcagtttgg
      301 ctctctgacc cacactcatc tgggcaaccc cttcttccca gcctcggatc acctcctgct
      361 tgcctagcat aaacttaaag ggcttgtttc tgtcccggga ggaatcgact ttctttccat
      421 cttcaagcat cccggtgtag tgcaccacac aggtctggcc gcgcttgggg aaggtgcgcc
      481 cgtctcctgg ggagatggtt tccacctgca ctcctctaga tatctcgagt gcggccgcga
      541 attcggtacc ggatccagtc gactgaattg gttcctttaa agcctgcttt tttgtacaaa
      601 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      661 caaaataaaa tcattnattt g
//
