LOCUS       sequence-259007-       12706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGreen3 CRISPR/Cas9 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
  TITLE     A Novel Ternary Vector System United with Morphogenic Genes Enhances
            CRISPR/Cas Delivery in Maize.
  JOURNAL   Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 
            10.1104/pp.19.00767.
  PUBMED    31558579
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767."
COMMENT     Sequence Label: pG3B-U6SC
FEATURES             Location/Qualifiers
     source          1..12706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        700..804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(805..873,874..1665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1023..1042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_RNA        1814..1889
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2865..2930
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2937..2957
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2982..7082
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2982..7082
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6995..7003
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7083..7130
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(7787..7803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7787..7803)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7800..7822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7811..7827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7835..7841,7842..7859,7860..7865))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7880..7901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8092..8768
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     8705..8725
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8813..9364
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    9371..9545
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9623..9647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10025..10044)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10036..10845
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10103..10122)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      10924..11010
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11102..11129
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      11208..11796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11697..11716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      11911..12521
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      11998..12529
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
      301 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
      361 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
      421 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
      481 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
      541 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
      601 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
      661 gagtcgaagt agtgattgtg agaccttcgg ggaaatgtgc gcggaacccc tatttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgggac aataaccctg ataaatgctt
      781 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      961 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1021 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1081 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1141 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1201 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1261 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1321 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1381 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1441 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1501 tctggagccg gtgagcgtgg ctctcgcggt atcattgcag cactggggcc agatggtaag
     1561 ccctcccgta tcgtagttat ctacaccacg gggagtcagg caactatgga tgaacgaaat
     1621 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1681 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1741 aagatccttt ttgataatcc catgaccaaa atcccttaac gtgagttttc gttccactga
     1801 gcgtcaggtc tcagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     1861 aacttgaaaa agtggcaccg agtcggtgct tttttttgga tagaatttcc cagctttttt
     1921 gcgtgtttca gctctcatga tccttgcatg cactagtatg gagtcaaaga ttcaaataga
     1981 ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact
     2041 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa
     2101 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
     2161 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     2221 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     2281 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     2341 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg attggcagac
     2401 atactgtccc acaaatgaag atggaatctg taaaagaaaa cgcgtgaaat aatgcgtctg
     2461 acaaaggtta ggtcggctgc ctttaatcaa taccaaagtg gtccctacca cgatggaaaa
     2521 actgtgcagt cggtttggct ttttctgacg aacaaataag attcgtggcc gacaggtggg
     2581 ggtccaccat gtgaaggcat cttcagactc caataatgga gcaatgacgt aagggcttac
     2641 gaaataagta agggtagttt gggaaatgtc cactcacccg tcagtctata aatacttagc
     2701 ccctccctca ttgttaaggg agcaaaatct cagagagata gtcctagaga gagaaagaga
     2761 gcaagtagcc tagaagtagt caaggcggcg aagtattcag gcacgtggcc aggaagaaga
     2821 aaagccaaga cgacgaaaac aggtaagagc taagcatcta gatggattac aaggaccacg
     2881 acggggatta caaggaccac gacattgatt acaaggatga tgatgacaag atggctccga
     2941 agaagaagag gaaggttggc atccacgggg tgccagctgc tgacaagaag tactcgatcg
     3001 gcctcgatat tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc
     3061 cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca
     3121 ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg
     3181 cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca
     3241 acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg
     3301 aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct
     3361 accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata
     3421 aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt
     3481 tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc
     3541 tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg
     3601 cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc
     3661 agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc
     3721 tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca
     3781 aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg
     3841 acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg
     3901 tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc
     3961 accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca
     4021 aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct
     4081 ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg
     4141 agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg
     4201 gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg
     4261 acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga
     4321 tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca
     4381 agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg
     4441 ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc
     4501 tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga
     4561 agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca
     4621 tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact
     4681 acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca
     4741 acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg
     4801 acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg
     4861 accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca
     4921 tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca
     4981 acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct
     5041 tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata
     5101 tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg
     5161 ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg
     5221 tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc
     5281 agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca
     5341 ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga
     5401 atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc
     5461 tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga
     5521 aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg
     5581 ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga
     5641 atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc
     5701 tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca
     5761 ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca
     5821 agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga
     5881 aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt
     5941 acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt
     6001 tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg
     6061 agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga
     6121 cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg
     6181 agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct
     6241 ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg
     6301 agtcaattct gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc
     6361 cgaagaagta cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga
     6421 aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga
     6481 ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca
     6541 aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga
     6601 acggcaggaa gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc
     6661 tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca
     6721 gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg
     6781 agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg
     6841 ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga
     6901 atatcattca tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg
     6961 acacaactat cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca
     7021 tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg
     7081 acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag tgagctcaga
     7141 gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt
     7201 gcgcacacac cagaatccta ctgagtttga gtattatggc attgggaaaa ctgtttttct
     7261 tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact
     7321 gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca
     7381 aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga
     7441 tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt
     7501 taatatgagg agtaaaacac ttgtagttgt accattatgc ttattcacta ggcaacaaat
     7561 atattttcag acctagaaaa gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt
     7621 ttagacattt atgaactttc ctttatgtaa ttttccagaa tccttgtcag attctaatca
     7681 ttgctttata attatagtta tactcatgga tttgtagttg agtatgaaaa tattttttaa
     7741 tgcattttat gacttgccaa ttgattgaca acgaattcgt aatcatgtca tagctgtttc
     7801 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7861 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7921 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7981 ggagaggcgg tttgcgtatt ggctagagca gcttgccaac atggtggagc acgacactct
     8041 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
     8101 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     8161 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
     8221 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     8281 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     8341 tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     8401 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     8461 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     8521 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
     8581 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     8641 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     8701 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     8761 cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagtcta ccatgagccc
     8821 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac
     8881 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc
     8941 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga
     9001 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta
     9061 cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg
     9121 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt
     9181 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc
     9241 cccacgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt
     9301 ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat
     9361 ttgactcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
     9421 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     9481 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     9541 agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     9601 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     9661 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     9721 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     9781 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     9841 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg gctggcaaac
     9901 agctattatg ggtattatgg gtctagtctc tggcccgtgt ctcaaaatct ctgatgttac
     9961 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
    10021 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
    10081 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
    10141 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
    10201 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
    10261 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
    10321 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
    10381 gaaaatattg ttgatgcgct ggcagtgtcc ctgcgccggt tgcattcgat tcctgtttgt
    10441 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
    10501 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
    10561 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
    10621 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
    10681 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
    10741 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
    10801 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
    10861 tggttgtaac attattcaga ttggcgtcta actactaagc gagagtaggg aactgccagg
    10921 catcaaataa aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg
    10981 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgtgaag
    11041 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaactaag
    11101 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc tgttagggcc
    11161 ccgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    11221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11881 tctgtggata accgtattac cgctagatag ctggttgccc tcgccgctgg gctggcggcc
    11941 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccccggc
    12001 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
    12061 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
    12121 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
    12181 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
    12241 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
    12301 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
    12361 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
    12421 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
    12481 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggtcgagtc atggtctagc
    12541 atggatctcg gggacgtcac ccataatacc cataatagct gtttgccagt actttgatcc
    12601 cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
    12661 gcccttttaa atatccgtta ttctaataaa cgctcttttc tcttag
//
