LOCUS       sequence-25901-e         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Degron, N-term, frame A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-FKBP12DD N-term A (B3-1)
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(95..97,98..418)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     protein_bind    complement(467..566)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagcaggctc caccatggga gtgcaggtgg aaaccatctc
      121 cccaggagac gggcgcacct tccccaagcg cggccagacc tgtgtggtgc actacaccgg
      181 gatgcttgaa gatggaaaga aagtcgattc ctcccgggac agaaacaagc cctttaagtt
      241 tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt
      301 gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc
      361 aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aaccggaagt
      421 cgactggatc cggtaccgaa ttcgcggccg cactcgagat atctagaccc agctttcttg
      481 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
      541 atcagtcaaa ataaaatcat tatttg
//
