LOCUS       sequence-259011-       13154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGreen3 CRISPR/Cas9 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
  TITLE     A Novel Ternary Vector System United with Morphogenic Genes Enhances
            CRISPR/Cas Delivery in Maize.
  JOURNAL   Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 
            10.1104/pp.19.00767.
  PUBMED    31558579
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767."
COMMENT     Sequence Label: pG3B-U6EC1
FEATURES             Location/Qualifiers
     source          1..13154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        700..804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(805..873,874..1665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1023..1042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_RNA        1814..1889
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3313..3378
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3385..3405
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3430..7530
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3430..7530
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7443..7451
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7531..7578
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(8235..8251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8235..8251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8248..8270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8259..8275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8283..8289,8290..8307,8308..8313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8328..8349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8540..9216
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9153..9173
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9261..9812
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    9819..9993
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10071..10095
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10473..10492)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10484..11293
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10551..10570)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      11372..11458
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11550..11577
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      11656..12244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12145..12164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      12359..12969
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      12446..12977
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
      301 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
      361 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
      421 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
      481 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
      541 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
      601 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
      661 gagtcgaagt agtgattgtg agaccttcgg ggaaatgtgc gcggaacccc tatttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgggac aataaccctg ataaatgctt
      781 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      961 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1021 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1081 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1141 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1201 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1261 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1321 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1381 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1441 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1501 tctggagccg gtgagcgtgg ctctcgcggt atcattgcag cactggggcc agatggtaag
     1561 ccctcccgta tcgtagttat ctacaccacg gggagtcagg caactatgga tgaacgaaat
     1621 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1681 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1741 aagatccttt ttgataatcc catgaccaaa atcccttaac gtgagttttc gttccactga
     1801 gcgtcaggtc tcagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     1861 aacttgaaaa agtggcaccg agtcggtgct tttttttgga tagaatttcc cagctttttt
     1921 gcgtgtttca gctctcatga tccttactag tgaataaaag catttgcgtt tggtttatca
     1981 ttgcgtttat acaaggacag agatccactg agctggaata gcttaaaacc attatcagaa
     2041 caaaataaac cattttttgt taagaatcag agcatagtaa acaacagaaa caacctaaga
     2101 gaggtaactt gtccaagaag atagctaatt atatctattt tataaaagtt atcatagttt
     2161 gtaagtcaca aaagatgcaa ataacagaga aactaggaga cttgagaata tacattcttg
     2221 tatatttgta ttcgagattg tgaaaatttg accataagtt taaattctta aaaagatata
     2281 tctgatctag gtgatggtta tagactgtaa ttttaccaca tgtttaatga tggatagtga
     2341 cacacatgac acatcgacaa cactatagca tcttatttag attacaacat gaaatttttc
     2401 tgtaatacat gtctttgtac ataatttaaa agtaattcct aagaaatata tttatacaag
     2461 gagtttaaag aaaacatagc ataaagttca atgagtagta aaaaccatat acagtatata
     2521 gcataaagtt caatgagttt attacaaaag cattggttca ctttctgtaa cacgacgtta
     2581 aaccttcgtc tccaatagga gcgctactga ttcaacatgc caatatatac taaatacgtt
     2641 tctacagtca aatgctttaa cgtttcatga ttaagtgact atttaccgtc aatcctttcc
     2701 cattcctccc actaatccaa ctttttaatt actcttaaat caccactaag ctagtaacgc
     2761 ctatcatgaa ttagctctac taaatctagc aacctttcaa atttgcagta ttgcaggtgt
     2821 ctctgtgtct ttaaaatagt tgccttatga tttcttcggt ttcaagatga tcaaatagtt
     2881 atagatttca tgctcacaca tgctcattag atgtgtacat actttactta cccaaatcta
     2941 ttttctcgca aagattttga tggtaaagct gatttggttc tattgaacta aatcaaacga
     3001 gtttcagact gagtgattct aatccggccc attagcccct aaacagaccc actaattacg
     3061 cagcttttaa tagagtaatt acacctagtt tacccactaa accactaagc actaattatc
     3121 tcacaatcta atgagcttcc ctcgtaatta cttgggcttt cactctacca tttatttgta
     3181 acagtcaagt ctctactgtc tctatataaa ctctctaaag ttaacacaca attctcatca
     3241 caaacaaatc aaccaaagca acttctactc tttcttcttt cgaccttatc aatctgttga
     3301 gaaatctaga tggattacaa ggaccacgac ggggattaca aggaccacga cattgattac
     3361 aaggatgatg atgacaagat ggctccgaag aagaagagga aggttggcat ccacggggtg
     3421 ccagctgctg acaagaagta ctcgatcggc ctcgatattg ggactaactc tgttggctgg
     3481 gccgtgatca ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc
     3541 gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg
     3601 gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc
     3661 atctgctacc tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc
     3721 cacaggctgg aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc
     3781 ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg
     3841 cggaagaagc tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg
     3901 gcccacatga ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat
     3961 agcgatgttg acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag
     4021 aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag
     4081 tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc
     4141 gggaatctca ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc
     4201 gctgaggacg ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc
     4261 ctggcccaga tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac
     4321 gccatcctcc tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca
     4381 gcctccatga tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg
     4441 gtcaggcagc agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc
     4501 tacgctgggt acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg
     4561 attctggaga agatggacgg cacggaggag ctgctggtga agctcaatcg cgaggacctc
     4621 ctgaggaagc agcggacatt cgataacggc agcatcccac accagattca tctcggggag
     4681 ctgcacgcta tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag
     4741 aagatcgaga agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc
     4801 aactcccgct tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc
     4861 gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc
     4921 gacaagaacc tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc
     4981 acagtctaca acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct
     5041 ttcctgtctg gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag
     5101 gtcacggtta agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc
     5161 gagatctctg gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg
     5221 aagatcatta aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac
     5281 attgtgctga cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact
     5341 tacgcccatc tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc
     5401 tgggggaggc tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg
     5461 atcctcgact tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac
     5521 gatgacagcc tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac
     5581 tcgctgcacg agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg
     5641 cagaccgtga aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac
     5701 atcgtcattg agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc
     5761 gagaggatga agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag
     5821 cacccggtgg agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat
     5881 ggccgcgata tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc
     5941 gatcatatcg ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc
     6001 aggtcggaca agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag
     6061 atgaagaact actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat
     6121 aacctcacaa aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag
     6181 aggcagctgg tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg
     6241 atgaacacta agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg
     6301 aagtcaaagc tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc
     6361 aacaattacc accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc
     6421 aagaagtacc caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg
     6481 aggaagatga tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc
     6541 tactctaaca ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg
     6601 aagcgccccc tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg
     6661 gatttcgcga ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca
     6721 gaggtccaga ctggcgggtt ctctaaggag tcaattctgc ctaagcggaa cagcgacaag
     6781 ctcatcgccc gcaagaagga ctgggatccg aagaagtacg gcgggttcga cagccccact
     6841 gtggcctact cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag
     6901 agcgtgaagg agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg
     6961 atcgatttcc tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc
     7021 cccaagtact cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctggc
     7081 gagctgcaga aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg
     7141 gcctcccact acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc
     7201 gtcgagcagc acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag
     7261 cgcgtgatcc tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc
     7321 gacaagccaa tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc
     7381 ggcgcccctg ctgctttcaa gtacttcgac acaactatcg atcgcaagag gtacacaagc
     7441 actaaggagg tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg
     7501 cgcatcgacc tgtctcagct cgggggcgac aagcggccag cggcgacgaa gaaggcgggg
     7561 caggcgaaga agaagaagtg agctcagagc tttcgttcgt atcatcggtt tcgacaacgt
     7621 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt
     7681 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     7741 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     7801 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     7861 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     7921 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     7981 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     8041 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     8101 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     8161 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac
     8221 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
     8461 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     8521 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     8581 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     8641 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     8701 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     8761 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
     8821 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     8881 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     8941 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     9001 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     9061 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     9121 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     9181 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     9241 ctatctctct cgagtctacc atgagcccag aacgacgccc ggccgacatc cgccgtgcca
     9301 ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg
     9361 tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc
     9421 gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg
     9481 cgggcccctg gaaggcacgc aacgcctacg actggacggc cgagtcgacc gtgtacgtct
     9541 ccccccgcca ccagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc
     9601 tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg
     9661 tgcgcatgca cgaggcgctc ggatatgccc cacgcggcat gctgcgggcg gccggcttca
     9721 agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtaccgc
     9781 cccgtccggt cctgcccgtc accgagattt gactcgagtt tctccataat aatgtgtgag
     9841 tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata
     9901 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     9961 ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag
    10021 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    10081 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    10141 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    10201 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    10261 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    10321 ctgcctgtga tcaccgcggc tggcaaacag ctattatggg tattatgggt ctagtctctg
    10381 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
    10441 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
    10501 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
    10561 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
    10621 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
    10681 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
    10741 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
    10801 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgtccct
    10861 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
    10921 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
    10981 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
    11041 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
    11101 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
    11161 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
    11221 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
    11281 tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt ggcgtctaac
    11341 tactaagcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga
    11401 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
    11461 gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcggg caggacgccc
    11521 gccataaact gccaggcatc aaactaagca gaaggccatc ctgacggatg gcctttttgc
    11581 gtttctacaa actcttcctg ttagggcccc gttccactga gcgtcagacc ccgtagaaaa
    11641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11761 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    11941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    12001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    12061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ctagatagct
    12361 ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc
    12421 gtcgaagccg tgtgcgagac accccggccg ccggcgttgt ggatacctcg cggaaaactt
    12481 ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc
    12541 ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc
    12601 gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct
    12661 ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc
    12721 gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt
    12781 tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg
    12841 ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa
    12901 ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg
    12961 aaccctcccg gtcgagtcat ggtctagcat ggatctcggg gacgtcaccc ataataccca
    13021 taatagctgt ttgccagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    13081 aaatggacga acggataaac cttttcacgc ccttttaaat atccgttatt ctaataaacg
    13141 ctcttttctc ttag
//
