LOCUS       sequence-259015-        6003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  detection/assessment of OCT4 and SOX2 transcription factor activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6003)
  TITLE     luciferase reporter plasmid containing 6 tandem copies of the 
            composite Oct4/Sox2 binding site of the FGF4 enhancer. Used for 
            detection of active Oct4/Sox2 complexes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 6xO/S luc
FEATURES             Location/Qualifiers
     source          1..6003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..58
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     42..58
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             485..2137
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(551..569)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2378..2443
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2573..2593
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3018..3152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3294..3311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3465..4053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3545..3564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4224..5015,5016..5084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4847..4866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5085..5189)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5216..5671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5303..5322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5513..5534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5861..5982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ataacccggg aggtaccgag ctccaccgcg gtggcggccg ctctagaact agtggatctg
       61 aaaactcttt gtttggatgc taatgggata cttaaaggat ctgaaaactc tttgtttgga
      121 tgctaatggg atacttaaag gatctgaaaa ctctttgttt ggatgctaat gggatactta
      181 aaggatctga aaactctttg tttggatgct aatgggatac ttaaaggatc tgaaaactct
      241 ttgtttggat gctaatggga tacttaaagg atcccccggg cgcggggcag gcgagtagga
      301 gggggcgccg gctatatata ccactgctcc ggagggctgg gcgcgcgggg actatcccgc
      361 caccgttgcg tccctatttg ctctcgctac ttaggtctgt gcgcagcact caccgaactc
      421 acggcccgca gctcgaactc acgcaagatc taagtaagct tggcattccg gtactgttgg
      481 taaaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga
      541 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      601 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
      661 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      721 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      781 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
      841 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
      901 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
      961 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     1021 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc
     1081 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
     1141 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     1201 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     1261 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga
     1321 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac
     1381 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct
     1441 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg
     1501 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     1561 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     1621 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc
     1681 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     1741 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa
     1801 gtctttaatt aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt
     1861 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga
     1921 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     1981 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     2041 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     2101 cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat tcagcgatga
     2161 cgaaattctt agctattgta atactgcgat gagtggcagg gcggggcgta atttttttaa
     2221 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
     2281 atgaatggca gaaattcgcc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     2341 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     2401 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     2461 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     2521 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     2581 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     2641 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     2701 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     2761 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     2821 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     2881 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     2941 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3001 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3121 aactcatcaa tgtatcttat catgtctgga tccgtcgacc gatgcccttg agagccttca
     3181 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     3241 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     3301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3901 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5281 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5341 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5401 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5521 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5581 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5641 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
     5701 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
     5761 gtaagtaata ttaaggtacg tggaggtttt acttgcttta aaaaacctcc cacacctccc
     5821 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5881 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5941 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatggtactg taactgagct
     6001 aac
//
