LOCUS       sequence-259027-       10862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Miyoshi H, Takahashi M, Gage FH, Verma IM
  TITLE     Stable and efficient gene transfer into the retina using an 
            HIV-based lentiviral vector.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Sep 16. 94(19):10319-23.
  PUBMED    9294208
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Sep 16. 94(19):10319-23."
COMMENT     Sequence Label: pHR' CMV GFP
FEATURES             Location/Qualifiers
     source          1..10862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2085..2388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2389..2592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2543..2563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2631..2640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2637..2639,2640..2642,2643..3353)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2637..2639,2640..2642,2643..3353)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2682..2703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2943..2962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3290..3311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3570..4203
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5682..5700)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5683..5700)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5990..6009)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6223..6242)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6342..6364
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6402..6420)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6488..6592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6593..6661,6662..7453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6811..6830)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7624..8212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8113..8132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8366..8383
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8453..8473)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8458..8787
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8638..8773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9921..9986
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10116..10136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10561..10695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10598..10617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10652..10671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatcc cccgggctgc aggaattcga ttaatagtaa
     2041 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2101 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2161 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2221 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2281 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2341 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2401 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2461 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2521 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2581 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg
     2641 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2701 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2761 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2821 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2881 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2941 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3001 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3061 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3121 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3241 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
     3361 tcagatccta ctagtaggat ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     3421 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     3481 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     3541 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     3601 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     3661 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     3721 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     3781 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     3841 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gagtacttca agaactgctg
     3901 acatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     3961 cgggactggg gagtggcgag ccctcagatg ctgcatataa gcagctgctt tttgcttgta
     4021 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4081 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4141 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4201 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     4261 cagagagtga gaggccttga cattataata gatttagcag gaattgaact aggagtggag
     4321 cacacaggca aagctgcaga agtacttgga agaagccacc agagatactc acgattctgc
     4381 acatacctgg ctaatcccag atcctaagga ttacattaag tttactaaca tttatataat
     4441 gatttatagt ttaaagtata aacttatcta atttactatt ctgacagata ttaattaatc
     4501 ctcaaatatc ataagagatg attactatta tccccattta acacaagagg aaactgagag
     4561 ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc gttagttacg actctatgat
     4621 cttctgacac aaattccatt tactcctcac cctatgactc agtcgaatat atcaaagtta
     4681 tggacattat gctaagtaac aaattaccct tttatatagt aaatactgag tagattgaga
     4741 gaagaaattg tttgcaaacc tgaatagctt caagaagaag agaagtgagg ataagaataa
     4801 cagttgtcat ttaacaagtt ttaacaagta acttggttag aaagggattc aaatgcataa
     4861 agcaagggat aaatttttct ggcaacaaga ctatacaata taaccttaaa tatgacttca
     4921 aataattgtt ggaacttgat aaaactaatt aaatattatt gaagattatc aatattataa
     4981 atgtaattta cttttaaaaa gggaacatag aaatgtgtat cattagagta gaaaacaatc
     5041 cttattatca caatttgtca aaacaagttt gttattaaca caagtagaat actgcattca
     5101 attaagttga ctgcagattt tgtgttttgt taaaattaga aagagataac aacaatttga
     5161 attattgaaa gtaacatgta aatagttcta catacgttct tttgacatct tgttcaatca
     5221 ttgatcgaag ttctttatct tggaagaatt tgttccaaag actctgaaat aaggaaaaca
     5281 atctattata tagtctcaca cctttgtttt acttttagtg atttcaattt aataatgtaa
     5341 atggttaaaa tttattcttc tctgagatca tttcacattg cagatagaaa acctgagact
     5401 ggggtaattt ttattaaaat ctaatttaat ctcagaaaca catctttatt ctaacatcaa
     5461 tttttccagt ttgatattat catataaagt cagccttcct catctgcagg ttccacaaca
     5521 aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa ttaaaaatag caatacaaca
     5581 ataaaaaaat acaaatcaga aaaacagcac agtataacaa ctttatttag catttacaat
     5641 ctattaggta ttataagtaa tctagaatta attccgtgta ttctatagtg tcacctaaat
     5701 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     5761 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     5821 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     5881 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     5941 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6001 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6061 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6121 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6181 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     6241 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6301 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     6361 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     6421 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6601 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6661 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6721 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6781 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6841 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6901 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6961 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7021 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7081 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7141 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7201 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7261 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7321 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7381 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7441 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7501 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8281 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8341 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8401 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     8461 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8521 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8581 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8641 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8701 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8761 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     8821 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     8881 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     8941 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     9001 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     9061 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     9121 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     9181 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     9241 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     9301 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     9361 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     9421 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     9481 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     9541 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     9601 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     9661 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     9721 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     9781 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     9841 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     9901 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     9961 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
    10021 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
    10081 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
    10141 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
    10201 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
    10261 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
    10321 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
    10381 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
    10441 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
    10501 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    10561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10681 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10741 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10801 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10861 gt
//
