LOCUS       sequence-259030-        9828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutively active 
            dominant-negative HSF1 fused to DHFR destabilized domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Moore CL, Dewal MB, Nekongo EE, Santiago S, Lu NB, Levine SS, 
            Shoulders MD
  TITLE     Transportable, Chemical Genetic Methodology for the Small 
            Molecule-Mediated Inhibition of Heat Shock Factor 1.
  JOURNAL   ACS Chem Biol. 2016 Jan 15;11(1):200-10. doi: 
            10.1021/acschembio.5b00740. Epub 2015 Nov 19.
  PUBMED    26502114
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00740"; journalName: "ACS Chem Biol"; date: 
            "2016-01-15- 15"; volume: "11"; issue: "1"; pages: "200-10"
COMMENT     Sequence Label: pLenti CMV Hygro DHFR.dn-cHSF1
FEATURES             Location/Qualifiers
     source          1..9828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4160..4463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4464..4667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4618..4638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4664..4688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4772..4796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5951..5975)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6006..6594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6059..6079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6477..6488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6650..7149
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6685..6708)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7082..7101
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7167..8192
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7167..8192
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             8566..8799
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8871..8992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8908..8927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8962..8981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9032..9167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9187..9206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9188..9206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9216..9233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9216..9232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9225..9247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9374..9802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9461..9480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9671..9692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     4681 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     4741 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa
     4801 aggaaccaat tcagtcgact ggatccggta ccaccatgga tctgcccgtg ggccccggcg
     4861 cggcggggcc cagcaacgtc ccggccttcc tgaccaagct gtggaccctc gtgagcgacc
     4921 cggacaccga cgcgctcatc tgctggagcc cgagcgggaa cagcttccac gtgttcgacc
     4981 agggccagtt tgccaaggag gtgctgccca agtacttcaa gcacaacaac atggccagct
     5041 tcgtgcggca gctcaacatg tatggcttcc ggaaagtggt ccacatcgag cagggcggcc
     5101 tggtcaagcc agagagagac gacacggagt tccagcaccc atgcttcctg cgtggccagg
     5161 agcagctcct tgagaacatc aagaggaaag tgaccagtgt gtccaccctg aagagtgaag
     5221 acataaagat ccgccaggac agcgtcacca agctgctgac ggacgtgcag ctgatgaagg
     5281 ggaagcagga gtgcatggac tccaagctcc tggccatgaa gcatgagaat gaggctctgt
     5341 ggcgggaggt ggccagcctt cggcagaagc atgcccagca acagaaagtc atcctggggg
     5401 tgaagagaaa gatccccctg atgctgaacg acagtggctc agcacattcc atgcccaagt
     5461 atagccggca gttctccctg gagcacgtcc acggctcggg cccctactcg gccccctccc
     5521 cagcctacag cagctccagc ctctacgccc ctgatgctgt ggccagctct ggacccatca
     5581 tctccgacat caccgagctg gctcctgcca gccccatggc ctcccccggc gggagcatag
     5641 acgagaggcc cctatccagc agccccctgg tgcgtgtcaa ggaggagccc cccagcccgc
     5701 ctcagagccc ccgggtagag gaggcgagtc ccgggcgccc atcttccgtg gacaccctct
     5761 tgtccccgac cgccctcatt gactccatcc tgcgggagag tgaacctgcc cccgcctccg
     5821 tcacagccct cacggacgcc aggggccaca cggacaccga gggccggcct ccctcccccc
     5881 cgcccacctc cacccctgaa aagtgcctca gcgtagcctg cctgtaggcg gccgcactcg
     5941 agatatctag acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     6001 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6241 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6421 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6601 ttctgcagat atccggacta gtgatctctc gaggttaacg aattctaccg ggtaggggag
     6661 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     6721 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     6781 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     6841 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     6901 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     6961 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     7021 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     7081 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     7141 ggcctttcga cctgcatccc gccaccatga aaaagcctga actcaccgcg acgtctgtcg
     7201 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     7261 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7321 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7381 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     7441 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     7501 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     7561 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     7621 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     7681 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     7741 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     7801 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     7861 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     7921 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     7981 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     8041 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     8101 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     8161 gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat
     8221 ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc gagagaacgg
     8281 ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc
     8341 gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc agggcacgat
     8401 tgtcatgccc acgcactcgg gtgatctgac tgatcccgca gattggagat cgccgcccgt
     8461 gcctgccgat tgggtgcaga tccgtcgagg gcccggtacc tttaagacca atgacttaca
     8521 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc
     8581 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8641 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8701 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8761 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8821 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9001 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9181 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9241 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9301 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9361 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatt
//
