LOCUS       sequence-259047-        8711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Borsos M, Perricone SM, Schauer T, Pontabry J, de Luca KL, de Vries 
            SS, Ruiz-Morales ER, Torres-Padilla ME, Kind J
  TITLE     Genome-lamina interactions are established de novo in the early 
            mouse embryo.
  JOURNAL   Nature. 2019 May;569(7758):729-733. doi: 10.1038/s41586-019-1233-0. 
            Epub 2019 May 22.
  PUBMED    31118510
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1233-0"; journalName: "Nature"; date: "2019-05";
            volume: "569"; issue: "7758"; pages: "729-733"
COMMENT     Sequence Label: Lenti_AID-Dam-only
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      515..524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             527..553
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             563..1243
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             2096..2137
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2147..2735
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2200..2220)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2618..2629)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2822..3055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3127..3248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3164..3183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3218..3237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3288..3423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3443..3462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3444..3462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3472..3489)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3472..3488)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3481..3503)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3630..4085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3717..3736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3927..3948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4111..4215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4216..4284,4285..5076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4434..4453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5247..5835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5736..5755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5989..6006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6123..6144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6159..6164,6165..6182,6183..6189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6197..6213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6202..6224
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6221..6237
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6221..6237
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6256..6276
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6258..6276
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        6377..6756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6757..6960
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6911..6931
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6975..7155
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7202..7327
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7820..8053
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8238..8282
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8549..8666
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggccgcc atgggctacc catacgatgt
      541 tcctgactat gcgggatcca atggcagtgt cgagctgaat ctgagggaga ctgagctgtg
      601 tcttggtctt cccggtggag atacagtggc tccggtaacc ggaaacaaga gagggttctc
      661 agagacggtt gatctgaagc taaatctgaa taatgagcct gcaaacaagg aaggatctac
      721 gactcatgac gtcgtgactt ttgattccaa ggagaagagt gcttgtccta aagatccagc
      781 caaacctccg gccaaggcac aagttgtggg atggccaccg gtgagatcat accggaagaa
      841 cgtgatggtt tcctgccaaa aatcaagcgg tggcccggag gcggcggcgt tcgtgaaggt
      901 atcaatggac ggagcaccgt acttgaggaa aatcgatttg aggatgtata aaagctacga
      961 tgagctttct aatgctttgt ccaacatgtt cagctctttt accatgggca aacatggagg
     1021 agaagaagga atgatagact tcatgaatga gaggaaattg atggatttgg tgaatagctg
     1081 ggactatgtt ccctcttatg aagacaaaga cggtgattgg atgctcgtcg gcgacgttcc
     1141 ttggccaatg ttcgtcgata catgcaagcg tttacgtctc atgaaaggat cggatgccat
     1201 tggtctcgct ccgagggcga tggagaagtg caagagcaga gctgcggcct ccaccatgaa
     1261 gaaaaatcgc gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa
     1321 acggcatttg cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt
     1381 tctcaacacc gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct
     1441 ctataacatt gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt
     1501 tcccgaaaca aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca
     1561 ggatccgttc cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct
     1621 gtgtcgttac aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta
     1681 tttcccggaa gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg
     1741 tgagtcttac gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc
     1801 gccttatgca ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac
     1861 gcttgaacaa caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc
     1921 agtgctgatc tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt
     1981 gcatgtcgtc aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga
     2041 cgaactgctg gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaaa ccggtggtaa
     2101 gcctatccct aaccctctcc tcggtctcga ttctacgtga gtcgacaatc aacctctgga
     2161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2521 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     2581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2701 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     2761 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     2821 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     2881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     3061 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     3121 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3241 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3301 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3361 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3421 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3481 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3541 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3601 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     3661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3841 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3901 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3961 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4021 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4081 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4141 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4441 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4921 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6241 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6301 tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca
     6361 acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     6421 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     6481 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     6541 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     6601 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     6661 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     6721 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     6781 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     6841 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     6901 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     6961 cgtttagtga accggggtct ctctggttag accagatctg agcctgggag ctctctggct
     7021 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7081 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7141 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7201 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7261 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7321 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7381 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7441 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7501 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7561 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7621 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7681 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7741 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7801 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7861 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7921 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7981 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8041 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8101 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8161 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8221 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8281 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8341 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8401 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8461 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8521 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     8581 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     8641 aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag aagcttgata
     8701 tcgaattcca c
//
