LOCUS       sequence-259053-       10065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutively active HSF1 fused to
            DHFR destabilized domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Phillips AM, Gonzalez LO, Nekongo EE, Ponomarenko AI, McHugh SM, 
            Butty VL, Levine SS, Lin YS, Mirny LA, Shoulders MD
  TITLE     Host proteostasis modulates influenza evolution.
  JOURNAL   Elife. 2017 Sep 26;6. doi: 10.7554/eLife.28652.
  PUBMED    28949290
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-26"; volume: "6. doi"; pages: " 10.7554/eLife.28652"
COMMENT     Sequence Label: pLenti CMV Puro DHFR.cHSF1
FEATURES             Location/Qualifiers
     source          1..10065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2349..2652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2653..2856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2807..2827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2853..2877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2961..2985
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3012..3488
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             4680..5051
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     protein_bind    complement(5078..5102)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5133..5721
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5186..5206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5604..5615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5735..6234
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5770..5793)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6167..6186
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6255..6854
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6255..6274)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6751..6771
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6992..7225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7297..7418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7334..7353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7388..7407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7458..7593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7613..7632)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7614..7632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7642..7659)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7642..7658)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7651..7673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7800..8255
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7887..7906)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8097..8118
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8281..8385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8386..8454,8455..9246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8604..8623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9417..10005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9906..9925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gtccagtgtg
     2941 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt
     3001 cagtcgacac catgatcagt ctgattgcgg cgttagcggt agattacgtt atcggcatgg
     3061 aaaacgccat gccgtggaac ctgcctgccg atctcgcctg gtttaaacgc aacaccttaa
     3121 ataaacccgt gattatgggc cgccatacct gggaatcaat cggtcgtccg ttgccaggac
     3181 gcaaaaatat tatcctcagc agtcaaccga gtacggacga tcgcgtaacg tgggtgaagt
     3241 cggtggatga agccatcgcg gcgtgtggtg acgtaccaga aatcatggtg attggcggcg
     3301 gtcgcgttat tgaacagttc ttgccaaaag cgcaaaaact gtatctgacg catatcgacg
     3361 cagaagtgga aggcgacacc catttcccgg attacgagcc ggatgactgg gaatcggtat
     3421 tcagcgaatt ccacgatgct gatgcgcaga actctcacag ctattgcttt gagattctgg
     3481 agcggcgagc aggaagtgga agtggatccg ccatggatct gcccgtgggc cccggcgcgg
     3541 cggggcccag caacgtcccg gccttcctga ccaagctgtg gaccctcgtg agcgacccgg
     3601 acaccgacgc gctcatctgc tggagcccga gcgggaacag cttccacgtg ttcgaccagg
     3661 gccagtttgc caaggaggtg ctgcccaagt acttcaagca caacaacatg gccagcttcg
     3721 tgcggcagct caacatgtat ggcttccgga aagtggtcca catcgagcag ggcggcctgg
     3781 tcaagccaga gagagacgac acggagttcc agcacccatg cttcctgcgt ggccaggagc
     3841 agctccttga gaacatcaag aggaaagtga ccagtgtgtc caccctgaag agtgaagaca
     3901 taaagatccg ccaggacagc gtcaccaagc tgctgacgga cgtgcagctg atgaagggga
     3961 agcaggagtg catggactcc aagctcctgg ccatgaagca tgagaatgag gctctgtggc
     4021 gggaggtggc cagccttcgg cagaagcatg cccagcaaca gaaagtcatc ctgggggtga
     4081 agagaaagat ccccctgatg ctgaacgaca gtggctcagc acattccatg cccaagtata
     4141 gccggcagtt ctccctggag cacgtccacg gctcgggccc ctactcggcc ccctccccag
     4201 cctacagcag ctccagcctc tacgcccctg atgctgtggc cagctctgga cccatcatct
     4261 ccgacatcac cgagctggct cctgccagcc ccatggcctc ccccggcggg agcatagacg
     4321 agaggcccct atccagcagc cccctggtgc gtgtcaagga ggagcccccc agcccgcctc
     4381 agagcccccg ggtagaggag gcgagtcccg ggcgcccatc ttccgtggac accctcttgt
     4441 ccccgaccgc cctcattgac tccatcctgc gggagagtga acctgccccc gcctccgtca
     4501 cagccctcac ggacgccagg ggccacacgg acaccgaggg ccggcctccc tcccccccgc
     4561 ccacctccac ccctgaaaag tgcctcagcg tagcctgcct ggacaagaat gagctcagtg
     4621 accacttgga tgctatggac tccaacctgg ataacctgca gaccatgctg agcagccacg
     4681 gcttcagcgt ggacaccagt gccctgctgg acctgttcag cccctcggtg accgtgcccg
     4741 acatgagcct gcctgacctt gacagcagcc tggccagtat ccaagagctc ctgtctcccc
     4801 aggagccccc caggcctccc gaggcagaga acagcagccc ggattcaggg aagcagctgg
     4861 tgcactacac agcgcagccg ctgttcctgc tggaccccgg ctccgtggac accgggagca
     4921 acgacctgcc ggtgctgttt gagctgggag agggctccta cttctccgaa ggggacggct
     4981 tcgccgagga ccccaccatc tccctgctga caggctcgga gcctcccaaa gccaaggacc
     5041 ccactgtctc ctaggcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtg
     5101 gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg
     5161 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5221 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5281 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5341 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5401 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5461 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5521 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5581 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5641 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5701 ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg
     5761 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     5821 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     5881 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     5941 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6001 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6061 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6121 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6181 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6241 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6301 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6361 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6421 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     6481 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     6541 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     6601 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     6661 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     6721 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     6781 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     6841 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     6901 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
     6961 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
     7021 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7081 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7141 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7201 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7261 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7381 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7441 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7501 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7561 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     7621 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7681 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7741 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7801 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7861 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7921 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7981 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8041 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8101 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8161 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8221 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     8281 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8341 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8401 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8461 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8521 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8581 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8641 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8701 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8761 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8821 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8881 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8941 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9001 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9061 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9121 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9181 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9241 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9301 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9361 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9541 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9661 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9721 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9781 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9841 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9961 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10021 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatg
//
