LOCUS       sequence-25906-e         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for N-term V5 tag, frame A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-V5 (w71-3)
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..187
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(257..298)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(310..409)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
       61 acacgggcca gagctgcagc tggatggcaa ataatgattt tattttgact gatagtgacc
      121 tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt gtacaagaaa
      181 gctgggtcta gatatctcga gtgcggccgc gaattcggta ccggatccag tcgactgaat
      241 tggttcccat ggatcccgta gaatcgagac cgaggagagg gttagggata ggcttacctt
      301 ccatggtgga gcctgctttt ttgtacaaag ttggcattat aaaaaagcat tgcttatcaa
      361 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg ggcccgagct
      421 taagtaacta tctaacagga agagtttgta gaaacgcaaa aaggccatcc gacgggacgg
//
