LOCUS       sequence-25908-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with EF1-a promoter, V5-His C-term, frame A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pEF-ENTR A (696-6)
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             147..188
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             198..215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(229..328)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(440..459)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(518..537)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gcttggtact aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc
       61 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
      121 cgctcgagtc tagagggccc ttcgaaggta agcctatccc taaccctctc ctcggtctcg
      181 attctacgcg taccggtcat catcaccatc accattgagt ttatctagac ccagctttct
      241 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      301 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
      361 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
      421 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
      481 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      541 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
      601 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
      661 aactggctga cggaatttat gcctcttccg ac
//
