LOCUS       sequence-259084-        1791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of SponGee, a buffer of cGMP that prevents 
            downstream signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ros O, Zagar Y, Ribes S, Baudet S, Loulier K, Couvet S, Ladarre D, 
            Aghaie A, Louail A, Petit C, Mechulam Y, Lenkei Z, Nicol X
  TITLE     SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP 
            Manipulation.
  JOURNAL   Cell Rep. 2019 Jun 25;27(13):4003-4012.e6. doi: 
            10.1016/j.celrep.2019.05.102.
  PUBMED    31242429
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.05"; journalName: "Cell Rep"; date: 
            "2019-06-25- 25"; volume: "27"; issue: "13"; pages: "4003-4012.e6"
COMMENT     Sequence Label: pCX-SponGee
FEATURES             Location/Qualifiers
     source          1..1791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1078..1087
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1084..1758
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRPS
                     TGA"
     primer_bind     complement(1214..1232)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1482..1501
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1676..1699
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggactaca aagacgatga cgacaagtcc ggaatcgata tgagcgagct ggaggaagac
       61 tttgccaaga ttctcatgct caaggaggag aggatcaaag agctggagaa gcggctgtca
      121 gagaaggagg aagaaatcca ggagctgaag aggaaactcc ataaatgcca gtcagtgctg
      181 cccgtgccct cgacccacat cggcccccgg accacccggg cacagggcat ctcggccgag
      241 ccgcagacct acaggtcctt ccacgacctc cgagtgaccc tgcccttcta ccccaagagt
      301 ccacagtcca agatcgatct cataaaggag gccatccttg acaatgactt tatgaagaac
      361 ttggagctgt cacagatcca agagattgtg gattgtatgt acccagtgga gtacggcaaa
      421 gacagctgca tcatcaaaga aggagatgtg gggtcactgg tgtatgtcat ggaagatggt
      481 aaggttgaag ttacaaaaga aggcgtgaag ctgtgcacaa tgggtcctgg taaagtgttt
      541 ggagagttgg ctatccttta caactgtacc cggacggcga ccgtcaaaac tcttgtaaat
      601 gtgaaactct gggccattga tcgacaatgt tttcagacga taatgatgag gacaggactt
      661 atcaagcata ccgagtatat ggaattttta aaaagcgttc caacattcca gagccttcct
      721 gaagagatcc tcagtaaact tgctgacgtc cttgaagaga cccactatga aaatggggaa
      781 tatatcatca ggcaaggtgc aagaggggac accttcttta tcatcagtaa aggaaaggtt
      841 aatgtcactc gtgaagactc gcccaatgaa gacccagtct ttcttagaac cttaggaaaa
      901 ggagattggt ttggagagaa agccttgcag ggggaagatg tgagaacagc gaatgtaatt
      961 gcggcagaag ctgtaacctg ccttgtgatc gacagagact ctttcaaaca tttgattgga
     1021 ggattagatg atgtttctaa taaagcatat gaagatgcag aagctaaggc ggatcccgcc
     1081 accatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
     1141 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1201 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     1261 atcctgtccc ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1321 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1381 gaggatggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1441 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1501 accatgggct gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1561 gagatcaaga tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc
     1621 acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg
     1681 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc
     1741 cgcccctcca ccggcgcctg tacaagtaag aattctgcag ataaacgcta g
//
