LOCUS       sequence-259087-        1806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of SponGee-Kras, a plasma membrane-targeted but
            lipid raft-excluded variant of the cGMP buffer SponGee that prevents
            downstream signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ros O, Zagar Y, Ribes S, Baudet S, Loulier K, Couvet S, Ladarre D, 
            Aghaie A, Louail A, Petit C, Mechulam Y, Lenkei Z, Nicol X
  TITLE     SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP 
            Manipulation.
  JOURNAL   Cell Rep. 2019 Jun 25;27(13):4003-4012.e6. doi: 
            10.1016/j.celrep.2019.05.102.
  PUBMED    31242429
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.05"; journalName: "Cell Rep"; date: 
            "2019-06-25- 25"; volume: "27"; issue: "13"; pages: "4003-4012.e6"
COMMENT     Sequence Label: pCX-SponGee-Kras
FEATURES             Location/Qualifiers
     source          1..1806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1081..1755
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRPS
                     TGA"
     primer_bind     complement(1211..1229)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1479..1498
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1673..1696
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gactacaaag acgatgacga caagtccgga atcgatatga gcgagctgga ggaagacttt
       61 gccaagattc tcatgctcaa ggaggagagg atcaaagagc tggagaagcg gctgtcagag
      121 aaggaggaag aaatccagga gctgaagagg aaactccata aatgccagtc agtgctgccc
      181 gtgccctcga cccacatcgg cccccggacc acccgggcac agggcatctc ggccgagccg
      241 cagacctaca ggtccttcca cgacctccga gtgaccctgc ccttctaccc caagagtcca
      301 cagtccaaga tcgatctcat aaaggaggcc atccttgaca atgactttat gaagaacttg
      361 gagctgtcac agatccaaga gattgtggat tgtatgtacc cagtggagta cggcaaagac
      421 agctgcatca tcaaagaagg agatgtgggg tcactggtgt atgtcatgga agatggtaag
      481 gttgaagtta caaaagaagg cgtgaagctg tgcacaatgg gtcctggtaa agtgtttgga
      541 gagttggcta tcctttacaa ctgtacccgg acggcgaccg tcaaaactct tgtaaatgtg
      601 aaactctggg ccattgatcg acaatgtttt cagacgataa tgatgaggac aggacttatc
      661 aagcataccg agtatatgga atttttaaaa agcgttccaa cattccagag ccttcctgaa
      721 gagatcctca gtaaacttgc tgacgtcctt gaagagaccc actatgaaaa tggggaatat
      781 atcatcaggc aaggtgcaag aggggacacc ttctttatca tcagtaaagg aaaggttaat
      841 gtcactcgtg aagactcgcc caatgaagac ccagtctttc ttagaacctt aggaaaagga
      901 gattggtttg gagagaaagc cttgcagggg gaagatgtga gaacagcgaa tgtaattgcg
      961 gcagaagctg taacctgcct tgtgatcgac agagactctt tcaaacattt gattggagga
     1021 ttagatgatg tttctaataa agcatatgaa gatgcagaag ctaaggcgga tcccgccacc
     1081 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
     1141 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1201 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     1261 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1321 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1381 gatggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1441 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1501 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
     1561 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
     1621 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
     1681 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
     1741 ccctccaccg gcgcctgtac aaagaagaag aagaagaaga gcaagaccaa gtgcgtgatc
     1801 atgtag
//
