LOCUS       sequence-259089-        1875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Lyn-SponGee, a lipid raft-targeted variant 
            of the cGMP buffer SponGee that prevents downstream signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ros O, Zagar Y, Ribes S, Baudet S, Loulier K, Couvet S, Ladarre D, 
            Aghaie A, Louail A, Petit C, Mechulam Y, Lenkei Z, Nicol X
  TITLE     SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP 
            Manipulation.
  JOURNAL   Cell Rep. 2019 Jun 25;27(13):4003-4012.e6. doi: 
            10.1016/j.celrep.2019.05.102.
  PUBMED    31242429
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.05"; journalName: "Cell Rep"; date: 
            "2019-06-25- 25"; volume: "27"; issue: "13"; pages: "4003-4012.e6"
COMMENT     Sequence Label: pCX-Lyn-SponGee
FEATURES             Location/Qualifiers
     source          1..1875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1162..1171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1168..1842
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRPS
                     TGA"
     primer_bind     complement(1298..1316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1566..1585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1760..1783
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgggctgca tcaagagcaa gcgcaaggac aagatgggct gcatcaagag caagcgcaag
       61 gacaaggcta gctccggagc caccatggac tacaaagacg atgacgacaa gtccggaatc
      121 gatatgagcg agctggagga agactttgcc aagattctca tgctcaagga ggagaggatc
      181 aaagagctgg agaagcggct gtcagagaag gaggaagaaa tccaggagct gaagaggaaa
      241 ctccataaat gccagtcagt gctgcccgtg ccctcgaccc acatcggccc ccggaccacc
      301 cgggcacagg gcatctcggc cgagccgcag acctacaggt ccttccacga cctccgagtg
      361 accctgccct tctaccccaa gagtccacag tccaagatcg atctcataaa ggaggccatc
      421 cttgacaatg actttatgaa gaacttggag ctgtcacaga tccaagagat tgtggattgt
      481 atgtacccag tggagtacgg caaagacagc tgcatcatca aagaaggaga tgtggggtca
      541 ctggtgtatg tcatggaaga tggtaaggtt gaagttacaa aagaaggcgt gaagctgtgc
      601 acaatgggtc ctggtaaagt gtttggagag ttggctatcc tttacaactg tacccggacg
      661 gcgaccgtca aaactcttgt aaatgtgaaa ctctgggcca ttgatcgaca atgttttcag
      721 acgataatga tgaggacagg acttatcaag cataccgagt atatggaatt tttaaaaagc
      781 gttccaacat tccagagcct tcctgaagag atcctcagta aacttgctga cgtccttgaa
      841 gagacccact atgaaaatgg ggaatatatc atcaggcaag gtgcaagagg ggacaccttc
      901 tttatcatca gtaaaggaaa ggttaatgtc actcgtgaag actcgcccaa tgaagaccca
      961 gtctttctta gaaccttagg aaaaggagat tggtttggag agaaagcctt gcagggggaa
     1021 gatgtgagaa cagcgaatgt aattgcggca gaagctgtaa cctgccttgt gatcgacaga
     1081 gactctttca aacatttgat tggaggatta gatgatgttt ctaataaagc atatgaagat
     1141 gcagaagcta aggcggatcc cgccaccatg gcctcctccg aggacgtcat caaggagttc
     1201 atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     1261 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     1321 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg ctccaaggcc
     1381 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     1441 aagtgggagc gcgtgatgaa cttcgaggat ggcggcgtgg tgaccgtgac ccaggactcc
     1501 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1561 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcggatgtac
     1621 cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc
     1681 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
     1741 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     1801 gaacagtacg agcgcgccga gggccgcccc tccaccggcg cctgtacaag taagaattct
     1861 gcagataaac gctag
//
