LOCUS       sequence-25909-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with EF1-a promoter, V5-His C-term, frame B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pEF-ENTR B (708-1)
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             153..194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             204..221
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(235..334)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(446..465)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(524..543)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta
       61 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg
      121 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
      181 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttat ctagacccag
      241 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
      301 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg
      361 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      421 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
      481 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      541 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
      601 gagttgtttc tgaaacatgg c
//
