LOCUS       sequence-25910-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with EF1-a promoter, V5-His C-term, frame C .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pEF-ENTR C (709-1)
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             145..186
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             196..213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(227..326)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(438..457)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(516..535)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta
       61 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg
      121 gccgctcgag gtcacccatt cgaaggtaag cctatcccta accctctcct cggtctcgat
      181 tctacgcgta ccggtcatca tcaccatcac cattgagttt atctagaccc agctttcttg
      241 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
      301 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa
      361 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
      421 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
      481 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
      541 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
      601 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
      661 ctggctgacg g
//
