LOCUS       sequence-25911-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pEF-ENTR-LacZ (710-1)
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(142..164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga cccaagctgg
       61 ctangtaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa ttctgcagat
      121 cgaaacgatg atagatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      181 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      241 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg
      301 gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga
      361 tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa
      421 cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg
      481 ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat
      541 ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg
      601 ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa
      661 ccgcctcgcg gtgaatggtg ctg
//
