LOCUS       sequence-25915-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV GFP Hygro (656-4)
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    32..620
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(85..105)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(503..514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgagctgtac aagtaaagcg gccgcgtcga caatcaacct ctggattaca aaatttgtga
       61 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      121 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      181 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      241 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
      301 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
      361 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
      421 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
      481 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
      541 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
      601 cctttgggcc gcctccccgc ctggaattct gcagatatcc ggactagtga tctctcgagg
      661 ttaacgaatt ctaccgggta ggggaggcgc ttttccca
//
