LOCUS       sequence-25916-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, CMV promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV GFP Neo (657-2)
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    36..624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(89..109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(507..518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(702..725)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tggacgagct gtacaagtaa agcggccgcg tcgacaatca acctctggat tacaaaattt
       61 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
      361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
      421 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
      481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
      541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
      601 tctccctttg ggccgcctcc ccgcctggaa ttctgcagat atccggacta gtgatctaat
      661 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
      721 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
      781 gtagg
//
