LOCUS       sequence-259221-       15162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B 
            along with luciferase enzyme for in vivo live imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15162)
  AUTHORS   Guha TK, Pichavant C, Calos MP
  TITLE     Plasmid-Mediated Gene Therapy in Mouse Models of Limb Girdle 
            Muscular Dystrophy.
  JOURNAL   Mol Ther Methods Clin Dev. 2019 Oct 14;15:294-304. doi: 
            10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13.
  PUBMED    31890729
REFERENCE   2  (bases 1 to 15162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2019-10-14"; volume: "15:294-304. doi"; 
            pages: " 10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13"
COMMENT     Sequence Label: pKLD-CAG-DYSF-CPL
FEATURES             Location/Qualifiers
     source          1..15162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    62..131
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        387..690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1334..1356
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1418..1437
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2171..2182
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7739..7795
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7796..8503
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7941..7959)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8209..8228
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8510..8563
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8570..9166
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8570..8589)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9066..9086
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9182..9238
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9239..10891
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(9305..9323)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(10968..10987)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11033..11088
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11087..11106)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11431..11450)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11475..11522
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    11693..11742
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     primer_bind     complement(11755..11772)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11761..11985
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             13392..14186
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             13392..14186
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13446..13465)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14056..14075
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      14511..15099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15000..15019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttatcttaag gtcgacgatg taggtcacgg
       61 tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
      121 cctcacccat ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga
      181 acgcgcggcg caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga
      241 gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct
      301 cgacggtcac ggcgggcatg tcgacctcga gattaatagt aatcaattac ggggtcatta
      361 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      421 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      721 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      781 tgtgcagcga tgggggcggg gggggggggg gkgkgtgcgt gggggggkga gcagggggtg
      841 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
      901 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
      961 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
     1021 ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     1081 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     1141 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     1201 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1261 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     1321 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1381 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     1441 tctcatcatt ttggcaaaga attcggtacc gtttaaacga tcggccgcca ccatgctgag
     1501 ggtcttcatc ctctatgccg agaacgtcca cacacccgac accgacatca gcgatgccta
     1561 ctgctccgcg gtgtttgcag gggtgaagaa gagaaccaaa gtcatcaaga acagcgtgaa
     1621 ccctgtatgg aatgagggat ttgaatggga cctcaagggc atccccctgg accagggctc
     1681 tgagcttcat gtggtggtca aagaccatga gacgatgggg aggaacaggt tcctggggga
     1741 agccaaggtc ccactccgag aggtcctcgc cacccctagt ctgtccgcca gcttcaatgc
     1801 ccccctgctg gacaccaaga agcagcccac aggggcctcg ctggtcctgc aggtgtccta
     1861 cacaccgctg cctggagctg tgcccctgtt cccgccccct actcctctgg agccctcccc
     1921 gactctgcct gacctggatg tagtggcaga cacaggagga gaggaagaca cagaggacca
     1981 gggactcact ggagatgagg cggagccatt cctggatcaa agcggaggcc cgggggctcc
     2041 caccacccca aggaaactac cttcacgtcc tccgccccac taccccggga tcaaaagaaa
     2101 gcgaagtgcg cctacatcta gaaagctgct gtcagacaaa ccgcaggatt tccagatcag
     2161 ggtccaggtg atcgaggggc gccagctgcc gggggtgaac atcaagcctg tggtcaaggt
     2221 taccgctgca gggcagacca agcggacgcg gatccacaag ggaaacagcc cactcttcaa
     2281 tgagactctt ttcttcaact tgtttgactc tcctggggag ctgtttgatg agcccatctt
     2341 tatcacggtg gtagactctc gttctctcag gacagatgct ctcctcgggg agttccggat
     2401 ggacgtgggc accatttaca gagagccccg gcacgcctat ctcaggaagt ggctgctgct
     2461 ctcagaccct gatgacttct ctgctggggc cagaggctac ctgaaaacaa gcctttgtgt
     2521 gctggggcct ggggacgaag cgcctctgga gagaaaagac ccctctgaag acaaggagga
     2581 cattgaaagc aacctgctcc ggcccacagg cgtagccctg cgaggagccc acttctgcct
     2641 gaaggtcttc cgggccgagg acttgccgca gatggacgat gccgtgatgg acaacgtgaa
     2701 acagatcttt ggcttcgaga gtaacaagaa gaacttggtg gacccctttg tggaggtcag
     2761 ctttgcgggg aaaatgctgt gcagcaagat cttggagaag acggccaacc ctcagtggaa
     2821 ccagaacatc acactgcctg ccatgtttcc ctccatgtgc gaaaaaatga ggattcgtat
     2881 catagactgg gaccgcctga ctcacaatga catcgtggct accacctacc tgagtatgtc
     2941 gaaaatctct gcccctggag gagaaataga agaggagcct gcaggtgctg tcaagccttc
     3001 gaaagcctca gacttggatg actacctggg cttcctcccc acttttgggc cctgctacat
     3061 caacctctat ggcagtccca gagagttcac aggcttccca gacccctaca cagagctcaa
     3121 cacaggcaag ggggaaggtg tggcttatcg tggccggctt ctgctctccc tggagaccaa
     3181 gctggtggag cacagtgaac agaaggtgga ggaccttcct gcggatgaca tcctccgggt
     3241 ggagaagtac cttaggaggc gcaagtactc cctgtttgcg gccttctact cagccaccat
     3301 gctgcaggat gtggatgatg ccatccagtt tgaggtcagc atcgggaact acgggaacaa
     3361 gttcgacatg acctgcctgc cgctggcctc caccactcag tacagccgtg cagtctttga
     3421 cgggtgccac tactactacc taccctgggg taacgtgaaa cctgtggtgg tgctgtcatc
     3481 ctactgggag gacatcagcc atagaatcga gactcagaac cagctgcttg ggattgctga
     3541 ccggctggaa gctggcctgg agcaggtcca cctggccctg aaggcgcagt gctccacgga
     3601 ggacgtggac tcgctggtgg ctcagctgac ggatgagctc atcgcaggct gcagccagcc
     3661 tctgggtgac atccatgaga caccctctgc cacccacctg gaccagtacc tgtaccagct
     3721 gcgcacccat cacctgagcc aaatcactga ggctgccctg gccctgaagc tcggccacag
     3781 tgagctccct gcagctctgg agcaggcgga ggactggctc ctgcgtctgc gtgccctggc
     3841 agaggagccc cagaacagcc tgccggacat cgtcatctgg atgctgcagg gagacaagcg
     3901 tgtggcatac cagcgggtgc ccgcccacca agtcctcttc tcccggcggg gtgccaacta
     3961 ctgtggcaag aattgtggga agctacagac aatctttctg aaatatccga tggagaaggt
     4021 gcctggcgcc cggatgccag tgcagatacg ggtcaagctg tggtttgggc tctctgtgga
     4081 tgagaaggag ttcaaccagt ttgctgaggg gaagctgtct gtctttgctg aaacctatga
     4141 gaacgagact aagttggccc ttgttgggaa ctggggcaca acgggcctca cctaccccaa
     4201 gttttctgac gtcacgggca agatcaagct acccaaggac agcttccgcc cctcggccgg
     4261 ctggacctgg gctggagatt ggttcgtgtg tccggagaag actctgctcc atgacatgga
     4321 cgccggtcac ctgagcttcg tggaagaggt gtttgagaac cagacccggc ttcccggagg
     4381 ccagtggatc tacatgagtg acaactacac cgatgtgaac ggggagaagg tgcttcccaa
     4441 ggatgacatt gagtgcccac tgggctggaa gtgggaagat gaggaatggt ccacagacct
     4501 caaccgggct gtcgatgagc aaggctggga gtatagcatc accatccccc cggagcggaa
     4561 gccgaagcac tgggtccctg ctgagaagat gtactacaca caccgacggc ggcgctgggt
     4621 gcgcctgcgc aggagggatc tcagccaaat ggaagcactg aaaaggcaca ggcaggcgga
     4681 ggcggagggc gagggctggg agtacgcctc tctttttggc tggaagttcc acctcgagta
     4741 ccgcaagaca gatgccttcc gccgccgccg ctggcgccgt cgcatggagc cactggagaa
     4801 gacggggcct gcagctgtgt ttgcccttga gggggccctg ggcggcgtga tggatgacaa
     4861 gagtgaagat tccatgtccg tctccacctt gagcttcggt gtgaacagac ccacgatttc
     4921 ctgcatattc gactatggga accgctacca tctacgctgc tacatgtacc aggcccggga
     4981 cctggctgcg atggacaagg actctttttc tgatccctat gccatcgtct ccttcctgca
     5041 ccagagccag aagacggtgg tggtgaagaa cacccttaac cccacctggg accagacgct
     5101 catcttctac gagatcgaga tctttggcga gccggccaca gttgctgagc aaccgcccag
     5161 cattgtggtg gagctgtacg accatgacac ttatggtgca gacgagttta tgggtcgctg
     5221 catctgtcaa ccgagtctgg aacggatgcc acggctggcc tggttcccac tgacgagggg
     5281 cagccagccg tcgggggagc tgctggcctc ttttgagctc atccagagag agaagccggc
     5341 catccaccat attcctggtt ttgaggtgca ggagacatca aggatcctgg atgagtctga
     5401 ggacacagac ctgccctacc caccacccca gagggaggcc aacatctaca tggttcctca
     5461 gaacatcaag ccagcgctcc agcgtaccgc catcgagatc ctggcatggg gcctgcggaa
     5521 catgaagagt taccagctgg ccaacatctc ctcccccagc ctcgtggtag agtgtggggg
     5581 ccagacggtg cagtcctgtg tcatcaggaa cctccggaag aaccccaact ttgacatctg
     5641 caccctcttc atggaagtga tgctgcccag ggaggagctc tactgccccc ccatcaccgt
     5701 caaggtcatc gataaccgcc agtttggccg ccggcctgtg gtgggccagt gtaccatccg
     5761 ctccctggag agcttcctgt gtgaccccta ctcggcggag agtccatccc cacagggtgg
     5821 cccagacgat gtgagcctac tcagtcctgg ggaagacgtg ctcatcgaca ttgatgacaa
     5881 ggagcccctc atccccatcc aggaggaaga gttcatcgat tggtggagca aattctttgc
     5941 ctccataggg gagagggaaa agtgcggctc ctacctggag aaggattttg acaccctgaa
     6001 ggtctatgac acacagctgg agaatgtgga ggcctttgag ggcctgtctg acttttgtaa
     6061 caccttcaag ctgtaccggg gcaagacgca ggaggagaca gaagatccat ctgtgattgg
     6121 tgaatttaag ggcctcttca aaatttatcc cctcccagaa gacccagcca tccccatgcc
     6181 cccaagacag ttccaccagc tggccgccca gggaccccag gagtgcttgg tccgtatcta
     6241 cattgtccga gcatttggcc tgcagcccaa ggaccccaat ggaaagtgtg atccttacat
     6301 caagatctcc atagggaaga aatcagtgag tgaccaggat aactacatcc cctgcacgct
     6361 ggagcccgta tttggaaaga tgttcgagct gacctgcact ctgcctctgg agaaggacct
     6421 aaagatcact ctctatgact atgacctcct ctccaaggac gaaaagatcg gtgagacggt
     6481 cgtcgacctg gagaacaggc tgctgtccaa gtttggggct cgctgtggac tcccacagac
     6541 ctactgtgtc tctggaccga accagtggcg ggaccagctc cgcccctccc agctcctcca
     6601 cctcttctgc cagcagcata gagtcaaggc acctgtgtac cggacagacc gtgtaatgtt
     6661 tcaggataaa gaatattcca ttgaagagat agaggctggc aggatcccaa acccacacct
     6721 gggcccagtg gaggagcgtc tggctctgca tgtgcttcag cagcagggcc tggtcccgga
     6781 gcacgtggag tcacggcccc tctacagccc cctgcagcca gacatcgagc aggggaagct
     6841 gcagatgtgg gtcgacctat ttccgaaggc cctggggcgg cctggacctc ccttcaacat
     6901 caccccacgg agagccagaa ggtttttcct gcgttgtatt atctggaata ccagagatgt
     6961 gatcctggat gacctgagcc tcacggggga gaagatgagc gacatttatg tgaaaggttg
     7021 gatgattggc tttgaagaac acaagcaaaa gacagacgtg cattatcgtt ccctgggagg
     7081 tgaaggcaac ttcaactgga ggttcatttt ccccttcgac tacctgccag ctgagcaagt
     7141 ctgtaccatt gccaagaagg atgccttctg gaggctggac aagactgaga gcaaaatccc
     7201 agcacgagtg gtgttccaga tctgggacaa tgacaagttc tcctttgatg attttctggg
     7261 ctccctgcag ctcgatctca accgcatgcc caagccagcc aagacagcca agaagtgctc
     7321 cttggaccag ctggatgatg ctttccaccc agaatggttt gtgtcccttt ttgagcagaa
     7381 aacagtgaag ggctggtggc cctgtgtagc agaagagggt gagaagaaaa tactggcggg
     7441 caagctggaa atgaccttgg agattgtagc agagagtgag catgaggagc ggcctgctgg
     7501 ccagggccgg gatgagccca acatgaaccc taagcttgag gacccaaggc gccccgacac
     7561 ctccttcctg tggtttacct ccccatacaa gaccatgaag ttcatcctgt ggcggcgttt
     7621 ccggtgggcc atcatcctct tcatcatcct cttcatcctg ctgctgttcc tggccatctt
     7681 catctacgcc ttcccgaact atgctgccat gaagctggtg aagcccttca gcacgcgtgc
     7741 cacgaacttc tctctgttaa agcaagcagg agacgtggaa gaaaaccccg gtcctatggt
     7801 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     7861 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     7921 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     7981 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     8041 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     8101 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     8161 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     8221 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     8281 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     8341 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     8401 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     8461 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagaccggtg agggcagagg
     8521 aagtcttcta acatgcggtg acgtggagga gaatcccggc ccttccggta tgaccgagta
     8581 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8641 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8701 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8761 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     8821 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8881 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8941 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9001 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9061 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9121 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccaccg gtggatccgg
     9181 tgccacgaac ttctctctgt taaagcaagc aggagacgtg gaagaaaacc ccggtcctat
     9241 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     9301 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     9361 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
     9421 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     9481 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     9541 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     9601 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     9661 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     9721 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     9781 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     9841 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     9901 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     9961 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
    10021 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
    10081 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
    10141 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
    10201 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
    10261 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
    10321 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
    10381 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
    10441 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
    10501 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
    10561 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
    10621 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
    10681 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
    10741 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
    10801 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
    10861 ggccaagaag ggcggaaaga tcgccgtgta agcggccgcg tgcaggctgc ctatcagaag
    10921 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
    10981 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
    11041 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
    11101 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
    11161 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
    11221 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
    11281 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
    11341 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
    11401 ctctctagag gatcctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    11461 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
    11521 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
    11581 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
    11641 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct cgtcggccgg
    11701 cttgtcgacg acggcggtct ccgtcgtcag gatcatccgg gccacacgcc tagccctcga
    11761 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    11821 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    11881 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    11941 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgggaatt caaactagag
    12001 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
    12061 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
    12121 agcgtgcggg gacagcccgg gcacggggaa ggtggcacag gatcgctttc ctctgaacgc
    12181 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct
    12241 gaaagagaga tttagaatga cagaatcata gaacggcctg ggttgcaaag gagcacagtg
    12301 ctcatccaga tccaaccccc tgctatgtgc agggtcatca accagcagcc caggctgccc
    12361 agagccacat ccagcctggc cttgaatgcc tgcagggatg gggcatccac agcctccttg
    12421 ggcaacctgt tcagtgcgtc accaccctct gggggaaaaa ctgcctcctc atatccaacc
    12481 caaacctccc ctgtctcagt gtaaagccat tcccccttgt cctatcaagg gggagtttgc
    12541 tgtgacattg ttggtctggg gtgacacatg tttgccaatt cagtgcatca cggagaggca
    12601 gatcttgggg ataaggaagt gcaggacagc atggacgtgg gacatgcagg tgttgagggc
    12661 tctgggacac tctccaagtc acagcgttca gaacagcctt aaagataaga agataggata
    12721 gaaggacaaa gagcaagtta aaacccagca tggagaggag cacaaaaagg ccacagacac
    12781 tgctggtccc tgtgtctgag cctgcatgtt tgatggtgtc tggatgcaag cagaaggggt
    12841 ggaagagctt gcctggagag atacagctgg gtcagtagga ctgggacagg cagctggaga
    12901 attgccatgt agatgttcat acaatcgtca aatcatgaag gctggaaaag ccctccaaga
    12961 tccccaagac caaccccaac ccacccaccg tgcccactgg ccatgtccct cagtgccaca
    13021 tccccacagt tcttcatcac ctccagggac ggtgaccccc ccacctccgt gggcagctgt
    13081 gccactgcag caccgctctt tggagaaggt aaatcttgct aaatccagcc cgaccctccc
    13141 ctggcacaac gtaaggccat tatctctcat ccaactccag gacggagtca gtgaggatgg
    13201 ggctctagtt tgcgcgcctc ttctactggg cggttttatg gacagcaagc gaaccggaat
    13261 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
    13321 tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
    13381 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
    13441 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
    13501 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
    13561 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
    13621 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
    13681 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
    13741 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    13801 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    13861 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    13921 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    13981 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggg gtggcggacc
    14041 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    14101 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    14161 atcgccttct tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt
    14221 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
    14281 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    14341 agacaataac cctgataaat gcttcaataa tagcacgtgc taaaacttca tttttaattt
    14401 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    14461 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    14521 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    14581 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    14641 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    14701 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    14761 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    14821 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    14881 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    14941 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    15001 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    15061 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    15121 ttacggttcc tggccttttg ctggcctttt gctcacatgt tc
//
