LOCUS       sequence-259247-        3456 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr mCherryDrTrkB expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pCMVd2-mCherry-Dr-TrkB
FEATURES             Location/Qualifiers
     source          1..3456
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(173..191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggatgta ttaaatcaaa aaggaaagac gctagcaagg gcgaggagga taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      301 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      361 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      541 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaaggaatt cagtgctggt agtgctggta gtgctggcac cggtatgagc
      781 cgggacccgt tgcccttttt tccaccgctt taccttggtg gcccggaaat taccaccgag
      841 aactgcgagc gcgagccgat tcatattccc ggcagcatcc agccgcacgg cgccctgctc
      901 actgccgacg ggcacagcgg cgaggtgctc cagatgagcc tcaacgcggc cacttttctg
      961 ggacaggaac ccacagtgct gcgcggacag accctcgccg cactgctgcc cgagcagtgg
     1021 cccgcgctgc aagcggccct gccccccggc tgccccgacg ccctgcaata ccgcgcaacg
     1081 ctggactggc ctgccgccgg gcacctttcg ctgacggtgc accgggtcgg cgagttgctg
     1141 attctggaat tcgagccgac ggaggcctgg gacagcaccg ggccgcacgc gctgcgcaac
     1201 gcgatgttcg cgctcgaaag tgcccccaac ctgcgggcgc tggccgaggt ggcgacccag
     1261 acggtccgcg agctgacggg ctttgaccgg gtgatgctct acaaatttgc ccccgacgcc
     1321 accggcgaag tgattgccga ggcccgccgt gaggggctgc acgcctttct gggccaccgt
     1381 tttcccgcgt cggacattcc ggcgcaggcc cgcgcgctct acacccggca cctgctgcgc
     1441 ctgaccgccg acacccgcgc cgccgccgtg ccgctcgatc ccgtcctcaa cccgcagacg
     1501 aatgcgccca ccccgctggg cggcgccgtg ctgcgcgcca cctcgcccat gcacatgcag
     1561 tacctgcgga acatgggcgt cgggtcgagc ctgtcggtgt cggtggtggt cggcggccag
     1621 ctctggggcc tgatcgcctg ccaccaccag acgccctacg tgttgccgcc cgacctgcga
     1681 accacgctcg aatacctggg ccgcttgctg agcctgcaag ttcaggtcaa ggaagcggcg
     1741 gacgtggcgg cctttcgcca gagcctgcgg gagcaccacg cgcgggtggc cctcgcggcg
     1801 gcgcactcgc tctcgccgca cgacaccctc agtgacccgg cgcttgacct gctgggcctg
     1861 atgcgggccg ggggcctgat tctgcgtttc gagggccgct ggcagacgtt gggtgaagtg
     1921 ccgcctgccc cggcggtgga cgcgctgctg gcgtggctcg aaacccagcc gggcgccctg
     1981 gtccagaccg acgcgctggg ccaactgtgg cccgccggcg ccgatctcgc ccccagcgca
     2041 gcgggcctgc tcgccatcag cgtgggcgag ggctggtcgg agtgcctcgt ctggctgcgg
     2101 cccgaactgc ggctggaggt cgcctggggc ggggccactc ctgaccaggc gaaagacgac
     2161 ctcgggccgc gccactcatt cgacacctac ctcgaagaaa aacgcggcta cgccgagccc
     2221 tggcatcccg gcgaaatcga ggaggcgcag gatctacgtg acacattgac cggggcgctg
     2281 ggcgaggcag aggcagcagc aaaggaggca gcagcaaagg aagcggcggc gaaagaagcg
     2341 gcggcgaaag cgaagttggc gagacattcc aagtttggca tgaaaggccc agcttccgtc
     2401 atcagcaacg acgatgactc tgccagccct ctccaccaca tctccaacgg gagcaacact
     2461 ccgtcttctt cggagggcgg gcccgatgct gtcatcattg ggatgaccaa gatccctgtc
     2521 attgaaaacc cccagtactt cggtatcacc aacagccagc tcaagccgga cacatttgtt
     2581 cagcacatca agagacacaa catcgttctg aagagggagc ttggagaagg agcctttggg
     2641 aaagttttcc tagcggagtg ctataacctc tgccccgagc aggataagat cctggtggcc
     2701 gtgaagacgc tgaaggacgc cagcgacaat gctcgcaagg actttcatcg cgaagccgag
     2761 ctgctgacca acctccagca cgagcacatt gtcaagttct acggtgtctg tgtggagggc
     2821 gacccactca tcatggtctt tgagtacatg aagcacgggg acctcaacaa gttccttagg
     2881 gcacacgggc cagatgcagt gctgatggca gagggtaacc cgcccaccga gctgacgcag
     2941 tcgcagatgc tgcacatcgc tcagcaaatc gcagcaggca tggtctacct ggcatcccaa
     3001 cacttcgtgc accgagacct ggccacccgg aactgcttgg taggagagaa cctgctggtg
     3061 aaaattgggg acttcgggat gtcccgggat gtatacagca ccgactacta ccgggttggt
     3121 ggccacacaa tgttgcccat ccgatggatg cctccagaga gcatcatgta caggaaattc
     3181 accaccgaga gtgacgtctg gagcctggga gttgtgttgt gggagatctt cacctacggc
     3241 aagcagccct ggtatcagct atcaaacaac gaggtgatag aatgcatcac ccagggcaga
     3301 gtccttcagc ggcctcgcac gtgtccccag gaggtgtacg agctgatgct gggatgctgg
     3361 cagcgggaac cacacacaag gaagaacatc aagaacatcc acacactcct tcagaacttg
     3421 gcgaaggcgt cgcccgtcta cctggacatc ctaggc
//
